| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97692-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90116-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27181-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47909-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54422-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26325-50ul | |||
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)
![Western Blot: PCYT1A Antibody [NBP2-97692] - Anti-PCYT1A rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Western-Blot-NBP2-97692-img0005.jpg "Western Blot: PCYT1A Antibody [NBP2-97692] - Anti-PCYT1A rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa")
![Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP2-97692] - Staining of human PCYT1A in human skin with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-97692-img0004.jpg)
![Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP2-97692] - Staining of human PCYT1A in human esophagus with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-97692-img0003.jpg)
![Immunoprecipitation: PCYT1A Antibody [NBP2-97692] - PCYT1A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-PCYT1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PCYT1A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunoprecipitation-NBP2-97692-img0006.jpg)
![Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Western-Blot-NBP2-90116-img0005.jpg "Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116] - Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Flow-Cytometry-NBP2-90116-img0004.jpg)
![Immunoprecipitation: CHIP/STUB1 Antibody (034) [NBP2-90116] - STUB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-STUB1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-STUB1 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Immunoprecipitation-NBP2-90116-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116] - Immunofluorescence staining of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90116 antibody (dilution ratio 1:300) overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90116_rabbit-monoclonal-chip-stub1-antibody-034-235202514331566.png)
![Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-28820241419150.jpg "Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-UBA3 antibody (NBP3-33072) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29820241420944.jpg)
![Immunocytochemistry/ Immunofluorescence: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunocytochemistry analysis of SiHa cells labeling UBA3 with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29202410334093.jpg)
![Western Blot: IMPDH1 Antibody (3G6) [H00003614-M01] - IMPDH1 monoclonal antibody (M01), clone 3G6. Western Blot analysis of IMPDH1 expression in HeLa [L013V1].](http://images.novusbio.com/fullsize/IMPDH1-Antibody-3G6-Western-Blot-H00003614-M01-img0008.jpg "Western Blot: IMPDH1 Antibody (3G6) [H00003614-M01] - IMPDH1 monoclonal antibody (M01), clone 3G6. Western Blot analysis of IMPDH1 expression in HeLa [L013V1].")
![Western Blot: IMPDH1 Antibody (3G6) [H00003614-M01] - IMPDH1 monoclonal antibody (M01), clone 3G6 Analysis of IMPDH1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/IMPDH1-Antibody-3G6-Western-Blot-H00003614-M01-img0006.jpg)
![ELISA: IMPDH1 Antibody (3G6) [H00003614-M01] - Detection limit for recombinant GST tagged IMPDH1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IMPDH1-Antibody-3G6-ELISA-H00003614-M01-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis of Hela cells using Hyaluronan synthase 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-1042024917038.jpg%3Fv=1666704794 "Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis of Hela cells using Hyaluronan synthase 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Western Blot: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis using Hyaluronan synthase 1 mAb against human Hyaluronan synthase 1 (AA: 74-399) recombinant protein. (Expected MW is 40.2 kDa)](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-1042024917022.jpg%3Fv=1666704794)
![Flow Cytometry: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis of SK-OV-3 cells using Hyaluronan synthase 1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-10420249141923.jpg%3Fv=1666704794)
![ELISA: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-10420249181917.jpg%3Fv=1666704794)
![Western Blot: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis using Hyaluronan synthase 1 mAb against HEK293 (1) and Hyaluronan synthase 1 (AA:74-399)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-1042024920246.jpg%3Fv=1666704794)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Placenta stained with CD34 MAb (QBEnd/10 + HPCA1/763).](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48362-img0005.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Placenta stained with CD34 MAb (QBEnd/10 + HPCA1/763).")
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Monoclonal Antibody (QBE.nd/10 + HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48362-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763).](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48362-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763).](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48362-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody [NBP2-27291] - Analysis of S1P1/EDG-1 in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right)](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27291-img0002.jpg "Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody [NBP2-27291] - Analysis of S1P1/EDG-1 in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right)")
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot Analysis of human Liver tissue lysate using Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-ALDH1A1-1381-Western-Blot-NBP2-53248-img0005.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot Analysis of human Liver tissue lysate using Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-ALDH1A1-1381-Immunohistochemistry-Paraffin-NBP2-53248-img0002.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot of K562 and Lung lysate Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-ALDH1A1-1381-Western-Blot-NBP2-53248-img0004.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-ALDH1A1-1381-Immunohistochemistry-Paraffin-NBP2-53248-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Tripeptidyl-Peptidase-I-TPP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31758-img0005.jpg "Immunocytochemistry/Immunofluorescence: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Mouse liver. TPP1 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Tripeptidyl-Peptidase-I-TPP1-Antibody-Immunohistochemistry-Paraffin-NBP1-31758-img0009.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tripeptidyl-Peptidase I/TPP1 antibody [N1C1] (NBP1-31758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31758_rabbit-polyclonal-tripeptidyl-peptidase-i-tpp1-antibody-western-blot-141120251833457.jpg)
![Immunohistochemistry: SIN3A Antibody (10G3) [NBP3-26325] - Image of SIN3A Antibody (10G3) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26325_rabbit-sin3a-mab-10g3-262202415475640.jpg "Immunohistochemistry: SIN3A Antibody (10G3) [NBP3-26325] - Image of SIN3A Antibody (10G3) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: SIN3A Antibody (10G3) [NBP3-26325] - Image of SIN3A Antibody (10G3) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26325_rabbit-sin3a-mab-10g3-262202415355736.jpg)
![Western Blot: SIN3A Antibody (10G3) [NBP3-26325] - Positive Western Blot detected in: K562 whole cell lysate, MCF-7 whole cell lysate. All lanes: SIN3A Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 146 kDa Observed band size: 146 kDa](http://images.novusbio.com/fullsize/nbp3-26325_rabbit-sin3a-mab-10g3-28220241152523.jpg)
![Immunocytochemistry/Immunofluorescence: SIN3A Antibody (10G3) [NBP3-26325] - Staining of HepG2 Cells with SIN3A Antibody (10G3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26325_rabbit-sin3a-mab-10g3-2822024131227.jpg)
![Western Blot: HEY1 Antibody [NBP2-27152] - Analysis of Hey1 in mouse embryo brain lysate using 1) preimmune sera at 1:5000 dilution and 2) purified NBP2-27152. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HEY1-Antibody-Western-Blot-NBP2-27152-img0002.jpg "Western Blot: HEY1 Antibody [NBP2-27152] - Analysis of Hey1 in mouse embryo brain lysate using 1) preimmune sera at 1:5000 dilution and 2) purified NBP2-27152. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")