| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26333-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24781SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89802-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50045SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB60071-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50035SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51590SS | |||
| Azide and BSA Free | NBP2-80874 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78966SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9355-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54488-100ug | |||
![Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-288202414191539.jpg "Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33093) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-29820241433367.jpg)
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33093, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-298202414304144.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-2920241114843.jpg)
![Flow Cytometry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33093, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-392024884612.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)
![Western Blot: Syntenin 1 Antibody (19C1) [NBP3-26333] - Positive Western Blot detected in: HT29 whole cell lysate, U87 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate. All lanes: Syntenin 1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-28220241147217.jpg)
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody (19C1) [NBP3-26333] - Staining of Hela with Syntenin 1 Antibody (19C1) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 521-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-28220241249364.jpg)
![Flow Cytometry: Syntenin 1 Antibody (19C1) [NBP3-26333] - Overlay Peak curve showing HepG2 cells stained with Syntenin 1 Antibody (19C1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-282202414312716.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - Western blot analysis of PGP9.5 / UCHL-1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using PGP9.5 / UCHL-1 antibody at 0.2 ug/ml.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NBP2-24781-img0005.jpg "Western Blot: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - Western blot analysis of PGP9.5 / UCHL-1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using PGP9.5 / UCHL-1 antibody at 0.2 ug/ml.")
![Immunohistochemistry-Paraffin: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - IHC analysis of human brain, cortex, using PGP9.5 / UCHL-1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24781-img0003.jpg)
![Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human stomach shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89802-img0016.jpg "Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human stomach shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89802-img0012.jpg)
![Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human cerebellum shows moderate to strong membranous positivity in neuropil of molecular layer and in Purkinje cell layer.](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89802-img0013.jpg)
![Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of rat and mouse tissue lysates using mouse ALDH1L1 mAb, dilution 1:5000 (Green): [1] protein standard (Red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-4A12-Western-Blot-NBP2-50045-img0006.jpg "Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of rat and mouse tissue lysates using mouse ALDH1L1 mAb, dilution 1:5000 (Green): [1] protein standard (Red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.")
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of mixed cortical neuron-glial cell culture from an E20 rat stained with mouse ALDH1L1 mAb, dilution 1:1,000 (Green), and costained with chicken Vimentin pAb, dilution 1:5000 (Red). DAPI staining of nuclear DNA (Blue). The ALDH1L1 antibody produces strong cytoplasmic staining of astrocytic glial cells, while the Vimentin antibody labels the intermediate filament cytoskeleton in fibroblasts and other glial cells. Some cells, presumably developing astrocytes, are stained by both ALDH1L1 and vimentin and so appear yellow.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-4A12-Immunocytochemistry-Immunofluorescence-NBP2-50045-img0005.jpg)
![Immunohistochemistry-Paraffin: UCH-L1 Antibody (972106) [MAB60071] - Antibody was detected in immersion fixed paraffin-embedded sections of human brain caudate nucleus tissue.](http://images.novusbio.com/fullsize/UCH-L1-Antibody-972106-Immunohistochemistry-Paraffin-MAB60071-img0001.jpg "Immunohistochemistry-Paraffin: UCH-L1 Antibody (972106) [MAB60071] - Antibody was detected in immersion fixed paraffin-embedded sections of human brain caudate nucleus tissue.")
![Simple Western: UCH-L1/PGP9.5 Antibody (972106) [MAB60071] - PC-12 cell lysate. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-972106-Simple-Western-MAB60071-img0002.jpg)
![Western Blot: MBP Antibody [NBP2-50035] - Analysis of different tissue lysates using chicken pAb to MBP, NBP2-50035, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, and [6] mouse spinal cord. Multiple bands between 14-21.5kDa correspond to various alternate transcripts of the single MBP gene.](http://images.novusbio.com/fullsize/MBP-Antibody-NBP2-50035-img0002.jpg "Western Blot: MBP Antibody [NBP2-50035] - Analysis of different tissue lysates using chicken pAb to MBP, NBP2-50035, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, and [6] mouse spinal cord. Multiple bands between 14-21.5kDa correspond to various alternate transcripts of the single MBP gene.")
![Immunohistochemistry: MBP Antibody [NBP2-50035] - Analysis of rat brain cerebellum section stained with chicken pAb to Myelin Basic Protein (MBP), NBP2-50035, dilution 1:5,000 in green, and costained with rabbit pAb to NF-L, NBP1-05218, dilution 1:5,000 in red. The MBP antibody stains oligodendrocytes and myelin sheathes around axons. NBP1-05218 antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/MBP-Antibody-NBP2-50035-img0001.jpg)
![Western Blot: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis using p75NTR/NGF Receptor mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Western-Blot-NBP1-51590-img0009.jpg "Western Blot: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis using p75NTR/NGF Receptor mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis of EC cells using p75NTR/NGF Receptor mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Immunocytochemistry-Immunofluorescence-NBP1-51590-img0008.jpg)
![Flow Cytometry: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis of EC cells using p75NTR/NGF Receptor mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Flow-Cytometry-NBP1-51590-img0007.jpg)
![Immunohistochemistry: TET1 Antibody [NBP1-78966] - Analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-NBP1-78966-img0001.jpg "Immunohistochemistry: TET1 Antibody [NBP1-78966] - Analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.")
![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Western Blot Analysis of human HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Western-Blot-NBP2-53236-img0007.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Western Blot Analysis of human HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1582).")
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta 3 Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Immunohistochemistry-Paraffin-NBP2-53236-img0005.jpg)
![Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Antibody (SPTBN2/1582). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Flow-Cytometry-NBP2-53236-img0004.jpg)
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Analysis of HeLa and 293 Cell Lysates using Spectrin beta III Monoclonal Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Western-Blot-NBP2-53236-img0001.jpg)
![Protein Array: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta 3 Antibody (SPTBN2/1582). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Protein-Array-NBP2-53236-img0006.jpg)