| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54544-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74814 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70511 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38305-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61843-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Western Blot: USP7 Antibody [NBP2-24641] - analysis of USP7 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/USP7-Antibody-Western-Blot-NBP2-24641-img0002.jpg "Western Blot: USP7 Antibody [NBP2-24641] - analysis of USP7 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: USP7 Antibody [NBP2-24641] - analysis of USP7 in formalin-fixed, paraffin-embedded human stomach tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NBP2-24641-img0001.jpg)
![Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB300-264-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB300-264-img0008.jpg)
![Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunoprecipitation-NB300-264-img0013.jpg)
![Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Western-Blot-NBP2-53358-img0004.jpg "Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (15B8) [NBP2-53358] - Confocal immunofluorescence image of HeLa cells using beta-Catenin Antibody (15B8) labeled is Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Immunocytochemistry-Immunofluorescence-NBP2-53358-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) [NBP2-53358] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (15B8).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Immunohistochemistry-Paraffin-NBP2-53358-img0001.jpg)
![Western Blot: Parkin Antibody (1H4) [H00005071-M01] - Parkin monoclonal antibody (M01), clone 1H4. Western Blot analysis of Parkin expression in PC-12.](http://images.novusbio.com/fullsize/Parkin-Antibody-1H4-Western-Blot-H00005071-M01-img0008.jpg "Western Blot: Parkin Antibody (1H4) [H00005071-M01] - Parkin monoclonal antibody (M01), clone 1H4. Western Blot analysis of Parkin expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (1H4) [H00005071-M01] - Analysis of monoclonal antibody to PARK2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-1H4-Immunocytochemistry-Immunofluorescence-H00005071-M01-img0004.jpg)
![Western Blot: Parkin Antibody (1H4) [H00005071-M01] - PARK2 monoclonal antibody (M01), clone 1H4 Analysis of PARK2 expression in Jurkat.](http://images.novusbio.com/fullsize/Parkin-Antibody-1H4-Western-Blot-H00005071-M01-img0006.jpg)
![ELISA: Parkin Antibody (1H4) [H00005071-M01] - Detection limit for recombinant GST tagged PARK2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Parkin-Antibody-1H4-ELISA-H00005071-M01-img0007.jpg)
![Western Blot: USP7 Antibody (1F12) [NBP2-01544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP7.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Western-Blot-NBP2-01544-img0002.jpg "Western Blot: USP7 Antibody (1F12) [NBP2-01544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP7.")
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human prostate tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0012.jpg)
![Flow Cytometry: USP7 Antibody (1F12) [NBP2-01544] - Analysis of Hela cells, using anti-USP7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Flow-Cytometry-NBP2-01544-img0001.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0004.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human colon tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0008.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human Kidney tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0010.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human pancreas tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0011.jpg)
![Western Blot: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Western-Blot-NBP2-46401-img0008.jpg "Western Blot: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0007.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0006.jpg)
![Western Blot: USP7 Antibody (3J7G1) [NBP3-16188] - Western blot analysis of extracts of various cell lines, using USP7 Rabbit mAb (NBP3-16188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/USP7-Antibody-3J7G1-Western-Blot-NBP3-16188-img0004.jpg "Western Blot: USP7 Antibody (3J7G1) [NBP3-16188] - Western blot analysis of extracts of various cell lines, using USP7 Rabbit mAb (NBP3-16188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using USP7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16188_rabbit-usp7-mab-3j7g1-5220251139223.jpg)
![Immunohistochemistry: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using USP7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16188_rabbit-usp7-mab-3j7g1-52202511383798.jpg)
![Immunohistochemistry: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using USP7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16188_rabbit-usp7-mab-3j7g1-52202511411481.jpg)
![Immunohistochemistry: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using USP7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16188_rabbit-usp7-mab-3j7g1-52202511392297.jpg)
![Immunohistochemistry: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using USP7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16188_rabbit-usp7-mab-3j7g1-522025125418.jpg)
![Immunohistochemistry: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using USP7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16188_rabbit-usp7-mab-3j7g1-5220251141147.jpg)
![Immunohistochemistry: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using USP7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16188_rabbit-usp7-mab-3j7g1-52202511383794.jpg)
![Immunohistochemistry: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using USP7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16188_rabbit-usp7-mab-3j7g1-52202511383783.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of NIH/3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411545558.jpg "Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of NIH/3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of A-549 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-101220241233811.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of U2OS cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411572633.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411531043.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-10122024120610.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Rat lung using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-101220241233849.jpg)
![Western Blot: NFkB p105/p50 Antibody [NBP3-38305] - Western blot analysis of lysates from Mouse lung, using NFkB p105/p50 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411572669.jpg)
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0005.jpg "Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of Hela cells using GRM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunocytochemistry-Immunofluorescence-NBP2-61843-img0003.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of cervical cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunohistochemistry-NBP2-61843-img0004.jpg)
![Flow Cytometry: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0002.jpg)
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0006.jpg)
![ELISA: mGluR3 Antibody (7A5A6) [NBP2-61843] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0001.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Western-Blot-NBP2-70511-img0001.jpg "Western Blot: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0007.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0006.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0008.jpg)