| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-00588SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73129 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61894-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82249-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80762-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84124-25ul | |||
![Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Western-Blot-H00005058-M02-img0011.jpg "Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on HeLa cell. Antibody concentration 35 ug/ml.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunocytochemistry-Immunofluorescence-H00005058-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunohistochemistry-Paraffin-H00005058-M02-img0008.jpg)
![Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Western Blot analysis of PAK1 expression in HeLa NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Western-Blot-H00005058-M02-img0001.jpg)
![Immunoprecipitation: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunoprecipitation-H00005058-M02-img0009.jpg)
![Sandwich ELISA: PAK1 Antibody (4D1) [H00005058-M02] - Detection limit for recombinant GST tagged PAK1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Sandwich-ELISA-H00005058-M02-img0010.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Western-Blot-NBP3-00588-img0003.jpg "Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NBP3-00588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Jumonji/JARID2 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00588-img0002.jpg)
![Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NBP3-00588] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Jumonji / JARID2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Jumonji / JARID2 antibody generated nuclear staining.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunohistochemistry-Paraffin-NBP3-00588-img0001.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI/Quaking protein stained by QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Frozen-NBP3-13575-img0005.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:3000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0001.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0002.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0003.jpg)
![QKI antibody [GT1589] detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: QKI protein stained by QKI antibody [GT1589] (NBP3-13575) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp3-13575_mouse-qki-quaking-mab-gt1589-immunocytochemistry-immunofluorescence-2611202518235322.jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025124758..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein by immunofluorescent analysis.Sample: DIV11 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.Red: MAP2, a microtubule marker, stained by MAP2 antibody [HM-2] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunohistochemistry-3042025123354..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein at cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunocytochemistry-immunofluorescence-3042025125627..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025125836..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513045..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513345..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513540..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025132854..jpg)
![<b>Orthogonal Strategies Validation. </b>Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] (NBP3-45160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-141120251831211.jpg)
![Tau antibody [HL2736] detects Tau protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: Tau stained by Tau antibody [HL2736] (NBP3-45160) diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-frozen-14112025185543.jpg)
![Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB .](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Western-Blot-NBP2-45877-img0017.jpg "Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB .")
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0016.jpg)
![Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Western-Blot-NBP2-45877-img0018.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0001.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human lung tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0002.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0003.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0004.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0005.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0006.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0007.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0008.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0009.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0010.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0011.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0012.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0013.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0014.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0015.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0007.jpg "Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0005.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0006.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mouse mAb against NIH/3T3 (1), U251 (2), C6 (3), A549 (4), and MCF-7 (5) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0004.jpg)
![ELISA: Synapsin I Antibody (7B1D9) [NBP2-61894] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-ELISA-NBP2-61894-img0001.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Analysis in human cerebral cortex and liver tissues. Corresponding STMN3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/STMN3-Antibody-Immunohistochemistry-Paraffin-NBP1-80779-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Analysis in human cerebral cortex and liver tissues. Corresponding STMN3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: STMN3 Antibody [NBP1-80779] - Analysis in control (vector only transfected HEK293T lysate) and STMN3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/STMN3-Antibody-Western-Blot-NBP1-80779-img0005.jpg)
![Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Staining of human liver shows no positivity in hepatocytes.](http://images.novusbio.com/fullsize/STMN3-Antibody-Immunohistochemistry-Paraffin-NBP1-80779-img0009.jpg)
![Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Staining of human testis shows no positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/STMN3-Antibody-Immunohistochemistry-Paraffin-NBP1-80779-img0007.jpg)
![Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/STMN3-Antibody-Immunohistochemistry-Paraffin-NBP1-80779-img0008.jpg)
![Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/STMN3-Antibody-Immunohistochemistry-Paraffin-NBP1-80779-img0010.jpg)
![Western Blot: Tau [p Thr231] Antibody [NB100-82249] - Western blot analysis of extracts from mouse brain tissue using NB100-82249 and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/Tau-[p-Thr231]-Antibody-Western-Blot-NB100-82249-img0002.jpg "Western Blot: Tau [p Thr231] Antibody [NB100-82249] - Western blot analysis of extracts from mouse brain tissue using NB100-82249 and the same antibody preincubated with blocking peptide.")
![Immunohistochemistry: Tau [p Thr231] Antibody [NB100-82249] - Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr231) antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr231]-Antibody-Immunohistochemistry-NB100-82249-img0001.jpg)
![Western Blot: EFHD1 Antibody (1H7) [H00080303-M05] - Analysis of EFHD1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/EFHD1-Antibody-1H7-Western-Blot-H00080303-M05-img0012.jpg "Western Blot: EFHD1 Antibody (1H7) [H00080303-M05] - Analysis of EFHD1 expression in Raw 264.7 (Cat # L024V1).")
![Immunocytochemistry/Immunofluorescence: EFHD1 Antibody (1H7) [H00080303-M05] - Analysis of monoclonal antibody to EFHD1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/EFHD1-Antibody-1H7-Immunocytochemistry-Immunofluorescence-H00080303-M05-img0008.jpg)
![Immunohistochemistry-Paraffin: EFHD1 Antibody (1H7) [H00080303-M05] - Analysis of monoclonal antibody to EFHD1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/EFHD1-Antibody-1H7-Immunohistochemistry-Paraffin-H00080303-M05-img0009.jpg)
![Western Blot: EFHD1 Antibody (1H7) [H00080303-M05] - Analysis of EFHD1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/EFHD1-Antibody-1H7-Western-Blot-H00080303-M05-img0010.jpg)
![Western Blot: EFHD1 Antibody (1H7) [H00080303-M05] - Analysis of EFHD1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/EFHD1-Antibody-1H7-Western-Blot-H00080303-M05-img0011.jpg)
![Western Blot: EFHD1 Antibody (1H7) [H00080303-M05] - Analysis of EFHD1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/EFHD1-Antibody-1H7-Western-Blot-H00080303-M05-img0013.jpg)
![Western Blot: Striatin Antibody [NBP1-80762] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NBP1-80762-img0011.jpg "Western Blot: Striatin Antibody [NBP1-80762] - Analysis in human cell line RH-30.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NBP1-80762] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80762-img0010.jpg)
![Immunohistochemistry-Paraffin: Striatin Antibody [NBP1-80762] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunohistochemistry-Paraffin-NBP1-80762-img0009.jpg)
![Western Blot: Striatin Antibody [NBP1-80762] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NBP1-80762-img0005.jpg)
![Western Blot: Striatin Antibody [NBP1-80762] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NBP1-80762-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SPAG16 Antibody [NBP1-84124] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/SPAG16-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84124-img0009.jpg "Immunocytochemistry/Immunofluorescence: SPAG16 Antibody [NBP1-84124] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol.")
