| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12918-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32020-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86684-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-20225-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-20224-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41301-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94811-0.02ml | |||
![Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Western-Blot-NBP2-12918-img0006.jpg "Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunocytochemistry-Immunofluorescence-NBP2-12918-img0008.jpg)
![Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunohistochemistry-NBP2-12918-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining in all cells. Some intermediate filamentlike staining in some cells.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunocytochemistry-Immunofluorescence-NBP2-12918-img0005.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0004.jpg "Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml")
![Immunohistochemistry: KCNN3 Antibody [NBP2-85137] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN3. Gene Name: KCNN3. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School](http://images.novusbio.com/fullsize/KCNN3-Antibody-Immunohistochemistry-NBP2-85137-img0006.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - WB Suggested Anti-KCNN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Daudi cell lysateKCNN3 is supported by BioGPS gene expression data to be expressed in Daudi](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0002.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - KCNN3 antibody - C-terminal region validated by WB using Mouse kidney, Whole brain lysate at 2 ug/ml.](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0003.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human 293T Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0005.jpg)
![Immunohistochemistry-Paraffin: KCNN3 Antibody [NBP2-85137] - Rabbit Anti-KCNN3 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult heart. Observed Staining: Membrane. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy2/3. Secondary Antibody Concentration: 1:200. Magnificatio](http://images.novusbio.com/fullsize/KCNN3-Antibody-Immunohistochemistry-Paraffin-NBP2-85137-img0001.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Analysis in human kidney and lymph node tissues. Corresponding SLC4A4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Analysis in human kidney and lymph node tissues. Corresponding SLC4A4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human pancreas shows strong membranous positivity in intercalated ducts.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0012.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human kidney shows strong cytoplasmic/ membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0010.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human lymph node shows no positivity as expected.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0011.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0013.jpg)
![Immunohistochemistry-Paraffin: Cav1.3 Antibody [NBP1-86684] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86684_rabbit-polyclonal-cav1-3-antibody-immunohistochemistry-paraffin-215202473422..jpg "Immunohistochemistry-Paraffin: Cav1.3 Antibody [NBP1-86684] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Cav1.3 Antibody [NBP1-86684] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86684_rabbit-polyclonal-cav1-3-antibody-immunohistochemistry-paraffin-215202473516..jpg)
![Western Blot: TRPV4 Antibody [NBP1-20225] - WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 at 1 : 1000 dilution.](http://images.novusbio.com/fullsize/TRPV4-Antibody-Western-Blot-NBP1-20225-img0003.jpg "Western Blot: TRPV4 Antibody [NBP1-20225] - WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 at 1 : 1000 dilution.")
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NBP1-20225] - IHC on paraffin sections of rat kidney: Animal was perfused with perfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin. HIER: 1 mM EDTA, pH 8 for 20 min. Detection was done using HRP polymer following manufactures instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NBP1-20225-img0002.jpg)
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NBP1-20225] - IHC on paraffin sections of rat kidney: Animal was perfused with perfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin. HIER: 1 mM EDTA, pH 8 for 20 min. Detection was done using HRP polymer following manufactures instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NBP1-20225-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20224-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained.")
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - IF on PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20224-img0004.jpg)
![Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - STX1A monoclonal antibody (M02), clone 1B11-1A8 Analysis of STX1A expression in IMR-32.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-Western-Blot-H00006804-M02-img0010.jpg "Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - STX1A monoclonal antibody (M02), clone 1B11-1A8 Analysis of STX1A expression in IMR-32.")
![Immunocytochemistry/Immunofluorescence: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Analysis of monoclonal antibody to STX1A on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-Immunocytochemistry-Immunofluorescence-H00006804-M02-img0007.jpg)
![Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - STX1A monoclonal antibody (M02), clone 1B11-1A8. Analysis of STX1A expression in PC-12.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-Western-Blot-H00006804-M02-img0009.jpg)
![Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Analysis of STX1A expression in transfected 293T cell line by STX1A monoclonal antibody (M02), clone 1B11-1A8.Lane 1: STX1A transfected lysate(29 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-Western-Blot-H00006804-M02-img0011.jpg)
![ELISA: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Detection limit for recombinant GST tagged STX1A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-ELISA-H00006804-M02-img0012.jpg)
![Western Blot: KCNK1 Antibody [NBP2-41301] - Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/KCNK1-Antibody-Western-Blot-NBP2-41301-img0001.jpg "Western Blot: KCNK1 Antibody [NBP2-41301] - Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 ug/ml.")
![Immunohistochemistry: KCNK1 Antibody - BSA Free [NBP2-41301] - Immunohistochemistry of KCNK1 in mouse brain tissue with KCNK1 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp2-41301_rabbit-polyclonal-kcnk1-antibody-2711202419541567.jpg)
![Immunocytochemistry/ Immunofluorescence: KCNK1 Antibody - BSA Free [NBP2-41301] - Immunofluorescence of KCNK1 in mouse brain tissue with KCNK1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41301_rabbit-polyclonal-kcnk1-antibody-2711202419313249.jpg)
![Western Blot: SNAP25 Antibody [NB100-1492] - (0.005ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NB100-1492-img0011.jpg "Western Blot: SNAP25 Antibody [NB100-1492] - (0.005ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-62000-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).")
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human prostate. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-62000-img0002.jpg)
![Flow Cytometry: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TRP1-Antibody-Flow-Cytometry-NBP2-62000-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-62000-img0004.jpg)
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human cortex. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-62000-img0001.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Western blot analysis of extracts of various cell lines, using Muscarinic Acetylcholine Receptor M1/CHRM1 antibody (NBP2-94811) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Western-Blot-NBP2-94811-img0006.jpg "Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Western blot analysis of extracts of various cell lines, using Muscarinic Acetylcholine Receptor M1/CHRM1 antibody (NBP2-94811) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Analysis of C6 cells using Muscarinic Acetylcholine Receptor M1/CHRM1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94811-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Analysis of NIH-3T3 cells using Muscarinic Acetylcholine Receptor M1/CHRM1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94811-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Analysis of OVCAR-3 cells using Muscarinic Acetylcholine Receptor M1/CHRM1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94811-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Analysis of OVCAR-3 cells using Muscarinic Acetylcholine Receptor M1/CHRM1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94811-img0003.jpg)