| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 647 | DDX0141A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50060SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50612-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94911-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05219-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45340 | |||
| Azide and BSA Free | NBP2-34807-0.1mg | |||
![Western Blot: GSTP1 Antibody [NBP2-16757] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/GSTP1-Antibody-Western-Blot-NBP2-16757-img0003.jpg "Western Blot: GSTP1 Antibody [NBP2-16757] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: GSTP1 Antibody [NBP2-16757] - 50 ug Rat brain lysate/extract 12 % SDS-PAGE GSTP1 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/GSTP1-Antibody-Western-Blot-NBP2-16757-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Immunocytochemistry-Immunofluorescence-DDX0141P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Flow-Cytometry-DDX0141P-100-img0002.jpg)
![Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Blocking-Neutralizing-DDX0141P-100-img0003.jpg)
![Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of tissue and cell lysates using rabbit pAb to MeCP2, NBP2-50060, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP2-50060-img0005.jpg "Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of tissue and cell lysates using rabbit pAb to MeCP2, NBP2-50060, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP2-50060] - Mouse brain section, which was cut at 45um on a vibratome after perfusion with 4% paraformaldehyde and overnight fixation, was stained with rabbit polyclonal antibody NBP2-50060. MeCP2 was mainly associated with methylated DNA in nucleus of neuron cells (green). Blue shows DAPI staining of nuclear DNA.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50060-img0003.jpg)
![Immunohistochemistry Free-Floating: MeCP2 Antibody [NBP2-50060] - Analysis of rat cerebellum section costained with rabbit pAb to MeCP2, NBP2-50060, dilution 1:5,000, in red, and chicken pAb to calbindin, dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50060-img0004.jpg)
![Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of MeCP2 expression in nuclear extracts from mouse brain with NBP2-50060. This polyclonal antibody recognizes a strong and clear band at 75 kDa corresponding to total MeCP2 of mouse brain in SDS-PAGE, while molecular weight of MeCP2 protein is 54 kDa.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP2-50060-img0002.jpg)
![Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NBP2-50612-img0002.jpg "Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP2-50612] - Immunofluorescent analysis of a section of mouse cerebellum stained with NBP2-50612 in green, and co-stained with a chicken polyclonal antibody to FOX2 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NF-L antibody labels dendrites and axons of neuronal cells predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including the Purkinje cells.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50612-img0001.jpg)
![Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Western-Blot-NBP3-15256-img0004.jpg "Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded mouse spinal cord using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0003.jpg)
![Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Western-Blot-NBP3-15256-img0005.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded rat brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0001.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded human brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0002.jpg)
![Immunohistochemistry: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CaM Kinase II Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15256_rabbit-cam-kinase-ii-mab-9p2e2-72202512443379.jpg)
![Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Western-Blot-NBP3-16285-img0003.jpg "Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Immunofluorescence analysis of U-2 OS cells using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunocytochemistry-Immunofluorescence-NBP3-16285-img0002.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody (2Q9K10) [NBP3-16285] - Human bladder cancer using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunohistochemistry-Paraffin-NBP3-16285-img0001.jpg)
![Immunoprecipitation: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of 300ug extracts of 293T cells using 3ug FTO antibody (NBP3-16285). Western blot was performed from the immunoprecipitate using FTO antibody (NBP3-16285) at a dilition of 1:500.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunoprecipitation-NBP3-16285-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Confocal imaging of U-2 OS cells using FTO Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16285_rabbit-fto-mab-2q9k10-52202511383732.jpg)
![Western Blot: SG2NA Antibody [NBP2-94911] - Analysis of extracts of various cell lines, using SG2NA . Exposure time: 10s.](http://images.novusbio.com/fullsize/SG2NA-Antibody-Western-Blot-NBP2-94911-img0005.jpg "Western Blot: SG2NA Antibody [NBP2-94911] - Analysis of extracts of various cell lines, using SG2NA . Exposure time: 10s.")
![Immunohistochemistry-Paraffin: SG2NA Antibody [NBP2-94911] - Paraffin-embedded rat kidney using SG2NA .](http://images.novusbio.com/fullsize/SG2NA-Antibody-Immunohistochemistry-Paraffin-NBP2-94911-img0004.jpg)
![Immunohistochemistry-Paraffin: SG2NA Antibody [NBP2-94911] - Paraffin-embedded human stomach using SG2NA .](http://images.novusbio.com/fullsize/SG2NA-Antibody-Immunohistochemistry-Paraffin-NBP2-94911-img0002.jpg)
![Immunohistochemistry-Paraffin: SG2NA Antibody [NBP2-94911] - Paraffin-embedded mouse lung using SG2NA .](http://images.novusbio.com/fullsize/SG2NA-Antibody-Immunohistochemistry-Paraffin-NBP2-94911-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: SG2NA Antibody - BSA Free [SG2NA] - Immunofluorescence analysis of HeLa cells using SG2NA Rabbit pAb . Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94911_rabbit-polyclonal-sg2na-antibody-52202516334142.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)
![Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody [NBP1-31209] - HeLa cells, using gamma Synuclein antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Synuclein-gamma-Antibody-Immunocytochemistry-NBP1-31209-img0006.jpg "Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody [NBP1-31209] - HeLa cells, using gamma Synuclein antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: gamma-Synuclein Antibody [NBP1-31209] - Mouse fore brain. gamma Synuclein antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Synuclein-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-31209-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: gamma-Synuclein Antibody [NBP1-31209] - Wild-type (WT) and gamma Synuclein knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma-Synuclein antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/gamma-Synuclein-Antibody-Western-Blot-NBP1-31209-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: gamma-Synuclein Antibody [NBP1-31209] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma Synuclein antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31209_rabbit-polyclonal-gamma-synuclein-antibody-1610202419405744.jpg)
![Western Blot: NF-L Antibody [NBP1-05219] - Analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.](http://images.novusbio.com/fullsize/NF-L-Antibody-NBP1-05219-img0006.jpg "Western Blot: NF-L Antibody [NBP1-05219] - Analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP1-05219] - View of mixed neuron/glial cultures stained with NBP1-05219 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05219-img0004.jpg)
![Immunohistochemistry Free-Floating: NF-L Antibody [NBP1-05219] - Staining of rat cerebellum section stained with chicken pAb to NF-L, NBP1-05219, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NBP1-05219 antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the FOX3/NeuN antibody stains the nuclei and proximal cytoplasm of neurons.](http://images.novusbio.com/fullsize/NF-L-Antibody-NBP1-05219-img0005.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Western Blot Analysis of human Brain lysate using UCH-L1/PGP9.5 Antibody (SPM574)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM574-Western-Blot-NBP2-32895-img0005.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Western Blot Analysis of human Brain lysate using UCH-L1/PGP9.5 Antibody (SPM574)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (SPM574) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM574-Immunocytochemistry-Immunofluorescence-NBP2-32895-img0004.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Formalin-fixed. paraffin-embedded rat cerebellum stained with Pgp9.5/UchL1 Monoclonal antibody (SPM574)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM574-Immunohistochemistry-Paraffin-NBP2-32895-img0003.jpg)