| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97747-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99063-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12711-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34405-0.1mg | |||
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-278202415212487.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-2982024149266.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-292024119233.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32389, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-29202416372194.jpg)
![Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP2-97747-img0003.jpg "Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP2-97747] - Immunofluorescence staining of ADNP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADNP polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97747-img0001.jpg)
![Immunoprecipitation: ADNP Antibody [NBP2-97747] - ADNP was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate. 4 ul anti-ADNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ADNP rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa. Observed band size: 150 kDa.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunoprecipitation-NBP2-97747-img0004.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-27820241631677.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32388) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-298202414161846.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-29202410234266.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32388, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-2920241645329.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-99063-img0003.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-99063-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-99063 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202514331592.png)
![Immunoprecipitation: Calbindin D-28K Antibody [NBP2-99063] - Calbindin D-28K was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 ul NBP2-99063 and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: NBP2-99063, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202516335024.png)
![Western Blot: UQCRB Antibody [NBP3-12711] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa](http://images.novusbio.com/fullsize/UQCRB-Antibody-Western-Blot-NBP3-12711-img0001.jpg "Western Blot: UQCRB Antibody [NBP3-12711] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa")
![Immunohistochemistry-Paraffin: UQCRB Antibody [NBP3-12711] - Staining of human UQCRB in human liver with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/UQCRB-Antibody-Immunohistochemistry-Paraffin-NBP3-12711-img0004.jpg)
![Immunohistochemistry-Paraffin: UQCRB Antibody [NBP3-12711] - Staining of human UQCRB in human kidney with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/UQCRB-Antibody-Immunohistochemistry-Paraffin-NBP3-12711-img0002.jpg)
![Immunohistochemistry-Paraffin: UQCRB Antibody [NBP3-12711] - Staining of human UQCRB in human colon carcinoma with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/UQCRB-Antibody-Immunohistochemistry-Paraffin-NBP3-12711-img0003.jpg)
![Immunoprecipitation: UQCRB Antibody [NBP3-12711] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-UQCRB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-UQCRB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size :14 kDa](http://images.novusbio.com/fullsize/UQCRB-Antibody-Immunoprecipitation-NBP3-12711-img0005.jpg)
![Western Blot: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-278202415242660.jpg "Western Blot: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32247) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-298202413585528.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Immunocytochemistry analysis of MCF7 cells labeling Cytokeratin 19 with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-29202410334065.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Flow cytometric analysis of MCF7 cells labeling Cytokeratin 19. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32247, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-39202488462.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody [NLS3218] - Analysis of anti-GRM3 / MGLUR3 antibody with transfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunocytochemistry-Immunofluorescence-NLS3218-img0010.jpg "Immunocytochemistry/Immunofluorescence: mGluR3 Antibody [NLS3218] - Analysis of anti-GRM3 / MGLUR3 antibody with transfected HEK293 human embryonic kidney cells.")
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS3218] - Anti-GRM3 / MGLUR3 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS3218-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody [NLS3218] - Analysis of anti-GRM3 / MGLUR3 antibody with untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunocytochemistry-Immunofluorescence-NLS3218-img0009.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS3218] - Analysis of anti-GRM3 / MGLUR3 antibody with human brain, neurons and glia at 9 ug/ml](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS3218-img0012.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0003.jpg "Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunocytochemistry-Immunofluorescence-NBP3-18258-img0001.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: cerebellum. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) alpha-Synuclein Antibody (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunohistochemistry-NBP3-18258-img0002.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0004.jpg)
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)
![Western Blot: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (SPM266).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-SPM266-Western-Blot-NBP2-34357-img0005.jpg "Western Blot: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (SPM266).")
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with cytokeratin 19 Monoclonal Antibody (SPM266).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-SPM266-Immunohistochemistry-Paraffin-NBP2-34357-img0003.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (SPM266) [NBP2-34357] - Flow Cytometric Analysis of Methanol-fixed MCF-7 cells. Cytokeratin 19 Antibody (SPM266) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-SPM266-Flow-Cytometry-NBP2-34357-img0004.jpg)