| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25146SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76792-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82245-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88959-25ul | |||
![Western Blot: BACE-1 Antibody [NBP1-62416] - Human Fetal Liver. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide.](http://images.novusbio.com/fullsize/BACE-1-Antibody-Western-Blot-NBP1-62416-img0017.jpg "Western Blot: BACE-1 Antibody [NBP1-62416] - Human Fetal Liver. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide.")
![Immunocytochemistry/Immunofluorescence: BACE-1 Antibody [NBP1-62416] - Mouse astrocytes (red fluorescence). Nuclei were stained with DAPI (blue fluorescence).](http://images.novusbio.com/fullsize/BACE-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-62416-img0012.jpg)
![Immunohistochemistry-Paraffin: BACE-1 Antibody [NBP1-62416] - Human testis tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/BACE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-62416-img0014.jpg)
![Western Blot: BACE-1 Antibody [NBP1-62416] - Sample Tissue: Human Fetal Liver, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane](http://images.novusbio.com/fullsize/BACE-1-Antibody-Western-Blot-NBP1-62416-img0011.jpg)
![Western Blot: BACE-1 Antibody [NBP1-62416] - Astrocytes, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/BACE-1-Antibody-Western-Blot-NBP1-62416-img0015.jpg)
![Western Blot: BACE-1 Antibody [NBP1-62416] - Mouse WT brain and Rat brain, concentration 2ug/ml.](http://images.novusbio.com/fullsize/BACE-1-Antibody-Western-Blot-NBP1-62416-img0016.jpg)
![Immunohistochemistry-Paraffin: BACE-1 Antibody [NBP1-62416] - Human adrenal tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/BACE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-62416-img0013.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Western blot analysis of Enolase 2/Neuron-specific Enolase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Western-Blot-NBP2-67641-img0007.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Western blot analysis of Enolase 2/Neuron-specific Enolase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur")
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Immunocytochemistry-Immunofluorescence-NBP2-67641-img0002.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Analysis of paraffin-embedded rat brain tissue using anti-NSE antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Immunohistochemistry-Paraffin-NBP2-67641-img0005.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Western blot analysis of Enolase 2/Neuron-specific Enolase on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was use](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Western-Blot-NBP2-67641-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Staining NSE in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Immunocytochemistry-Immunofluorescence-NBP2-67641-img0001.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Analysis of paraffin-embedded mouse brain tissue using anti-NSE antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Immunohistochemistry-Paraffin-NBP2-67641-img0004.jpg)
![Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - beta-amyloid (pyroglu11) NBP1-44070 in red (left) co-labeled with 6E10 in green (middle) and merge (right). Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu11]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44070-img0003.jpg "Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - beta-amyloid (pyroglu11) NBP1-44070 in red (left) co-labeled with 6E10 in green (middle) and merge (right). Image from verified customer review.")
![Western Blot: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - Lane 1: Ovalbumin Control. Lane 2: beta Amyloid pyro E11 peptide. Lane 3: beta Amyloid 11 peptide. Lane 4: beta Amyloid pyro E3 peptide. Load: 1 ug per lane. Primary antibody: Beta Amyloid pyro E11 antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E11 peptide.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu11]-Antibody-Western-Blot-NBP1-44070-img0008.jpg)
![Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE11Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44070 antibody.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu11]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44070-img0005.jpg)
![Western Blot: APP Antibody [NBP1-32081] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody, C-term diluted at 1:500.](http://images.novusbio.com/fullsize/APP-Antibody-Western-Blot-NBP1-32081-img0008.jpg "Western Blot: APP Antibody [NBP1-32081] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody, C-term diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: APP Antibody [NBP1-32081] - APP antibody, C-term detects APP protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APP protein stained by APP antibody, C-term diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/APP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32081-img0007.jpg)
![Immunohistochemistry-Paraffin: APP Antibody [NBP1-32081] - APP antibody, C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis. Sample: Paraffin-embedded Rat brain. APP antibody, C-term diluted at 1:500.](http://images.novusbio.com/fullsize/APP-Antibody-Immunohistochemistry-Paraffin-NBP1-32081-img0006.jpg)
![Western Blot: APP Antibody [NBP1-32081] - Sample(30 ug of whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-32081-img0005.jpg)
![Immunohistochemistry-Paraffin: APP Antibody [NBP1-32081] - -embedded Tonsil, plasma cells, using antibody 10 ug/ml.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-32081-img0003.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunofluorescence-NBP2-25146-img0010.jpg "Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.")
![Western Blot: alpha-Synuclein Antibody [NBP2-25146] - Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-25146-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 (NBP2-25156, green). The alpha-Synuclein antibody stains vesicular structures, the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-Synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 (1:1000) secondary antibody. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Image from verified customer review.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0006.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425483.jpg "Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-212024939175.jpg)
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249432853.jpg)
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425466.jpg)
![Immunocytochemistry/Immunofluorescence: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 10 min. Green: GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425437.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-212024940814.jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)Please notice that NBP3-45157 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45158.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)Please notice that NBP3-45158 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45157. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Tau (p Thr181) antibody [HL2497] detects Tau (p Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse brain.Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2497] (GTX638855) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg)
![Western Blot: Presenilin-1 Antibody [NBP1-76792] - Human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Western-Blot-NBP1-76792-img0002.jpg "Western Blot: Presenilin-1 Antibody [NBP1-76792] - Human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody [NBP1-76792] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) Presenilin-1. Overlay images indicate the possible co-localization of limited co-localization of VDR/ Presenilin-1 on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76792-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Presenilin-1 Antibody - BSA Free [NBP1-76792] - Immunofluorescence of Presenilin-1 in Human Brain cells with Presenilin-1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76792_rabbit-polyclonal-presenilin-1-antibody-2711202419201530.jpg)
![Immunohistochemistry: Presenilin-1 Antibody - BSA Free [NBP1-76792] - Immunohistochemistry of Presenilin-1 in human brain tissue with Presenilin-1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76792_rabbit-polyclonal-presenilin-1-antibody-271120242092064.jpg)
![Western Blot: PPP2R5D Antibody [NB100-846] - Staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NB100-846-img0002.jpg "Western Blot: PPP2R5D Antibody [NB100-846] - Staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PPP2R5D Antibody [NB100-846] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Immunocytochemistry-Immunofluorescence-NB100-846-img0003.jpg)
![Flow Cytometry: PPP2R5D Antibody [NB100-846] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Flow-Cytometry-NB100-846-img0004.jpg)
![Western Blot: PPP2R5D Antibody [NB100-846] - Staining of A431 (A), Jurkat (B) and HepG2 (C) cell lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NB100-846-img0005.jpg)
![Western Blot: Tau [p Thr181] Antibody [NB100-82245] - PPP2R2C level regulates Tau phosphatase levels. Representative image of immunoblots of indicated antibodies are shown in SHSY5Y cell lines after knockdown and overexpression PPP2R2C. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103048) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Tau [p Thr181] Antibody [NB100-82245] - PPP2R2C level regulates Tau phosphatase levels. Representative image of immunoblots of indicated antibodies are shown in SHSY5Y cell lines after knockdown and overexpression PPP2R2C. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103048) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Tau [p Thr181] Antibody [NB100-82245] - analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr181) antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Immunohistochemistry-Paraffin-NB100-82245-img0005.jpg)
![Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Analysis of extracts from mouse brain tissue using Tau(Phospho-Thr181) Antibody and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0001.jpg)
![Simple Western: Tau [p Thr181] Antibody [NB100-82245] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Simple-Western-NB100-82245-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PPP2R5D Antibody [NBP1-88959] - Analysis using Anti-PPP2R5D antibody NBP1-88959 (A) shows similar pattern to independent antibody NBP1-88960 (B).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NBP1-88959-img0009.jpg)
![Immunohistochemistry-Paraffin: PPP2R5D Antibody [NBP1-88959] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Immunohistochemistry-Paraffin-NBP1-88959-img0008.jpg)
![Western Blot: PPP2R5D Antibody [NBP1-88959] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NBP1-88959-img0006.jpg)
