| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22401-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37276-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59332-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22424-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59327-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42196-0.025mg | |||
![Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Western-Blot-NBP2-22401-img0003.jpg "Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.")
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Brevican Antibody (D) Composite.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Brevican Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0005.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0004.jpg "Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Immunocytochemistry-Immunofluorescence-NBP2-37276-img0005.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0002.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0003.jpg)
![ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-ELISA-NBP2-37276-img0001.jpg)
![Western Blot: Olig2 Antibody (1G11) [NBP2-37636] - Western blot analysis using OLIG2 mAb against HEK293 (1) and OLIG2(AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Olig2-Antibody-1G11-Western-Blot-NBP2-37636-img0002.jpg "Western Blot: Olig2 Antibody (1G11) [NBP2-37636] - Western blot analysis using OLIG2 mAb against HEK293 (1) and OLIG2(AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Olig2 Antibody (1G11) [NBP2-37636] - Immunofluorescence analysis of U251 cells using OLIG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Olig2-Antibody-1G11-Immunocytochemistry-Immunofluorescence-NBP2-37636-img0003.jpg)
![Immunohistochemistry: Olig2 Antibody (1G11) [NBP2-37636] - Immunohistochemical analysis of paraffin-embedded muscle tissues using OLIG2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Olig2-Antibody-1G11-Immunohistochemistry-NBP2-37636-img0004.jpg)
![ELISA: Olig2 Antibody (1G11) [NBP2-37636] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Olig2-Antibody-1G11-ELISA-NBP2-37636-img0001.jpg)
![Western Blot: Npas4 Antibody (S408-79) [NBP2-59332] - Western Blot analysis of Rat Brain showing detection of ~90 kDa Npas4 protein using Mouse Anti-Npas4 Monoclonal Antibody, Clone S408-79 (NBP2-59332). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Npas4 Monoclonal Antibody (NBP2-59332) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.](http://images.novusbio.com/fullsize/Npas4-Antibody-S408-79-Western-Blot-NBP2-59332-img0005.jpg "Western Blot: Npas4 Antibody (S408-79) [NBP2-59332] - Western Blot analysis of Rat Brain showing detection of ~90 kDa Npas4 protein using Mouse Anti-Npas4 Monoclonal Antibody, Clone S408-79 (NBP2-59332). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Npas4 Monoclonal Antibody (NBP2-59332) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.")
![Immunocytochemistry/Immunofluorescence: Npas4 Antibody (S408-79) [NBP2-59332] - Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Npas4-Antibody-S408-79-Immunocytochemistry-Immunofluorescence-NBP2-59332-img0004.jpg)
![Western Blot: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5/1 protein using Mouse Anti-mGluR5/1 Monoclonal Antibody, Clone S75-33 (NBP2-22424). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5/1 Monoclonal Antibody (NBP2-22424) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.](http://images.novusbio.com/fullsize/mGluR5-1-Antibody-S75-3-Western-Blot-NBP2-22424-img0002.jpg "Western Blot: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5/1 protein using Mouse Anti-mGluR5/1 Monoclonal Antibody, Clone S75-33 (NBP2-22424). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5/1 Monoclonal Antibody (NBP2-22424) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.")
![Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-3 (NBP2-22424). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (NBP2-22424) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) mGluR1/5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/mGluR5-1-Antibody-S75-3-Immunocytochemistry-Immunofluorescence-NBP2-22424-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody, Clone S75-3 (NBP2-22424). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody (NBP2-22424) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) mGluR1/5 glutamate receptor Antibody. (D) Composite.](http://images.novusbio.com/fullsize/mGluR5-1-Antibody-S75-3-Immunocytochemistry-Immunofluorescence-NBP2-22424-img0004.jpg)
![Western Blot: NMDAR1 Antibody (S308-48) [NBP2-59327] - Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48 (NBP2-59327). Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody (NBP2-59327) at 1:1000.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-S308-48-Western-Blot-NBP2-59327-img0004.jpg "Western Blot: NMDAR1 Antibody (S308-48) [NBP2-59327] - Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48 (NBP2-59327). Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody (NBP2-59327) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NMDAR1/NR1 Monoclonal Antibody, Clone S308-48 (NBP2-59327). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-NMDAR1/NR1 Monoclonal Antibody (NBP2-59327) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NMDAR1/NR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-S308-48-Immunocytochemistry-Immunofluorescence-NBP2-59327-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluN1/NR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-S308-48-Immunocytochemistry-Immunofluorescence-NBP2-59327-img0003.jpg)
![Western Blot: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Western Blot analysis of Rat Brain Membrane showing detection of 36-38 kDa QKI/Quaking (pan) protein using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 36-38 kDa.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Western-Blot-NBP2-42196-img0003.jpg "Western Blot: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Western Blot analysis of Rat Brain Membrane showing detection of 36-38 kDa QKI/Quaking (pan) protein using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 36-38 kDa.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI/Quaking (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42196-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42196-img0002.jpg)
![Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Western-Blot-NBP2-70558-img0001.jpg "Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Doublecortin.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70558-img0003.jpg)
![Flow Cytometry: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Analysis of Jurkat cells, using anti-Doublecortin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70558-img0002.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606] - Western blot showing a single band at the correct MW (47 kDa).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB100-1606-img0003.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606] - Western blot showing a single band at the correct MW (47 kDa).")
![Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-288202414191534.jpg "Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Vimentin antibody (NBP3-33092) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33092) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-298202414304186.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-2920241192398.jpg)
![Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-278202415295563.jpg "Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32501) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-298202413585569.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunocytochemistry analysis of NIH/3T3 cells labeling KIF3A with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-29202410334094.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-69992] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69992-img0002.jpg "Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-69992] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992] - (6ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NBP2-69992_Immunohistochemistry-Paraffin-Doublecortin-Antibody-NBP2-69992-6ug-ml-staining-of-paraffin-embedded-Human-Cortex-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254027.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.](http://images.novusbio.com/fullsize/NBP2-69992_Immunohistochemistry-Paraffin-Doublecortin-Antibody-NBP2-69992-Negative-Control-showing-staining-of-paraffin-embedded-Human-Cortex-with-no-primary-antibody--29320239254010.jpg)
![Flow Cytometry: Doublecortin Antibody [NBP2-69992] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Flow-Cytometry-NBP2-69992-img0001.jpg)