| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44244 | |||
| BSA Free | NBP2-61431-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90283-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44243 | |||
| BSA Free | NBP2-61430-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33076 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48002-0.1mg | |||
| IHC-Prediluted | NBP2-48186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47911-0.1mg | |||
| IHC-Prediluted | NBP2-48363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77179-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70854 | |||
![Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Western-Blot-NBP2-61431-img0010.jpg "Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of A549 cells. Nuclear probes are visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunocytochemistry-Immunofluorescence-NBP2-61431-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Western-Blot-NBP2-61431-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of SH-SY5Y cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunocytochemistry-Immunofluorescence-NBP2-61431-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human cerebellum shows strong positivity in both neuronal cell bodies and neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human prostate shows strong positivity in the peripheral nerves.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex, colon, epididymis and testis using Anti-GSS antibody NBP2-57177 (A) shows similar protein distribution across tissues to independent antibody NBP2-30465 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Glutathione Synthetase Antibody [NBP2-57177] - Analysis using Anti-GSS antibody NBP2-57177 (A) shows similar pattern to independent antibody NBP2-30465 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP2-57177-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57177-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human testis.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human colon.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human epididymis using Anti-GSS antibody NBP2-57177.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Filamin A Antibody [NBP1-90283] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90283-img0011.jpg "Immunocytochemistry/Immunofluorescence: Filamin A Antibody [NBP1-90283] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human kidney, liver, pancreas and testis using Anti-FLNA antibody NBP1-90283 (A) shows similar protein distribution across tissues to independent antibody NBP1-90284 (B).](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0013.jpg)
![Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human testis using Anti-FLNA antibody NBP1-90283.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0026.jpg)
![Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human testis shows moderate cytoplasmic positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0018.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Western-Blot-NBP2-61430-img0010.jpg "Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of A549 cells showing specific staining on microtubules in green. Nuclear probes are visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunocytochemistry-Immunofluorescence-NBP2-61430-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Western-Blot-NBP2-61430-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of SH-SY5Y cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunocytochemistry-Immunofluorescence-NBP2-61430-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human cerebellum shows strong positivity in both neurons and neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human prostate shows strong positivity in the peripheral nerves.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0007.jpg)
![Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Western-Blot-NBP2-37816-img0014.jpg "Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin in SHSY5Y cells (human neuroblast) either left untreated (left panel) or treated with 10uM retinoic acid (right panel) for 72 hours. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a beta-3 Tubulin monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37816-img0011.jpg)
![Immunohistochemistry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Normal biopsies of deparaffinized human brain tissues.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunohistochemistry-NBP2-37816-img0012.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin (blue histogram) on human SHSY5Y neuroblast cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a beta-3 Tubulin monoclonal antibody at a 1:50 dilution or PBS alone (red histogram) for 1 hour at room temperature. For flow cytometry analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody was performed and 10,000 cells were acquired for each sample.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Flow-Cytometry-NBP2-37816-img0010.jpg)
![Immunoprecipitation: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin was performed using SHSY5Y whole cell lysate. Antigen-antibody complexes were formed by incubating 300ug of lysate with 5ug of a beta-3 Tubulin monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. SHSY5Y cell lysate (30ug) was loaded as a positive control (left lane). The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a beta-3 Tubulin monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunoprecipitation-NBP2-37816-img0013.jpg)
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0002.jpg "Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.")
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0001.jpg)
![Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Western-Blot-NBP2-44975-img0006.jpg "Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (HSPB1/774) [NBP2-44975] - ICC/IF analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunofluorescence-NBP2-44975-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0002.jpg)
![Flow Cytometry: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Flow-Cytometry-NBP2-44975-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)")
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Flow-Cytometry-NBP2-44569-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0002.jpg)
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Percent of EPCs (CD133 + CD34 +) reduced in the XYK group detected by flow cytometry. Data are mean +/- SD analyzed by one-way analysis of variance. **P < 0.01. CSDH, chronic subdural hematoma; EPC, endothelial progenitor cell; XYK, Xiaoyukang Jiaonang. PE conjugated CD34 antibody used (NBP2-47911PE). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32328124/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-44569_mouse-monoclonal-cd34-antibody-hpca1-1171-177202495947.jpg)
![Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Western-Blot-NBP2-34621-img0002.jpg "Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34621-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34621-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0005.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.Red: ZEB2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202419333477.gif)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415392525.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415334920.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415395947.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241535644.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Functional domains of Fbxo45 for ubiquitination of Zeb2A. Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, & then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. B. Bacterially expressed GST or GST–Fbxo45, -Fbxo45 delta F-box, -Fbxo45 delta mid, or -Fbxo45 delta SPRY fusion proteins & Glutathione-Sepharose beads were incubated with lysates of HeLa cells transfected with Zeb2, Snai1 or Snai2, respectively. The proteins associated with GST–tagged Fbxo45 forms, bound on the Glutathione-Sepharose beads were washed five times with the RIPA buffer before immunoblotting. C. Lysates from 293T cells transfected Flag-Zeb2 with HA-Fbxo45, -Fbxo45 delta mid, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were immunoprecipitated with anti-Flag or anti-HA antibodies, & immunoprecipitates were resolved by SDS-PAGE for Western-blot analysis. D. U2OS cells transfected with Flag-Zeb2 & HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were stained using the primary antibodies of anti-Flag M2 or anti-HA, & the second antibodies of Alexa Fluor 568 anti-mouse or Alexa Fluor 488 anti-Rabbit, respectively. Scale: 25μm. E-I. Zeb2 ubiquitination assays by using IP expriments under different conditions: Flag-Zeb2 with or without HA-Ub (E); Flag-Zeb2 with HA-Ub WT, K48R or K63R (F); Flag-Zeb2 & HA-Ub K48-only with or without myc-Fbox45 (G); Flag-Zeb2 & HA-Ub K48-only with siRNA control, siRNAs for Fbxo45 or siRNAs for Pam (H); Flag-Zeb2 & HA-Ub with HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416163752.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416175234.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241616011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420285893.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420272496.jpg)
![Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Western-Blot-NBP2-03351-img0002.jpg "Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.")
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0005.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Flow-Cytometry-NBP2-03351-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0004.jpg)