| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60237-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15492-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94571-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03276-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22411-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22401-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89836-50ul | |||
![Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-4E5G9-Western-Blot-NBP3-15590-img0005.jpg "Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Caspase-9 Rabbit mAb (NBP3-15590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-4E5G9-Immunocytochemistry-Immunofluorescence-NBP3-15590-img0003.jpg)
![Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-4E5G9-Western-Blot-NBP3-15590-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Immunofluorescence analysis of C6 cells using [KO Validated] Caspase-9 Rabbit mAb (NBP3-15590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-4E5G9-Immunocytochemistry-Immunofluorescence-NBP3-15590-img0002.jpg)
![Western Blot: SCAMP4 Antibody [NBP2-81787] - Analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/SCAMP4-Antibody-Western-Blot-NBP2-81787-img0001.jpg "Western Blot: SCAMP4 Antibody [NBP2-81787] - Analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: SCAMP4 Antibody - BSA Free [NBP2-81787] - Immunofluorescence of SCAMP4 in Human Brain cells with SCAMP4 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81787_rabbit-polyclonal-scamp4-antibody-2711202420175159.jpg)
![Immunohistochemistry: SCAMP4 Antibody - BSA Free [NBP2-81787] - Immunohistochemistry of SCAMP4 in human brain tissue with SCAMP4 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81787_rabbit-polyclonal-scamp4-antibody-2711202420352425.jpg)
![Western Blot: MT-ND5 Antibody [NBP3-05604] - Western blot analysis of extracts of various cell lines, using MT-ND5 antibody (NBP3-05604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MT-ND5 Antibody-Western Blot-NBP3-05604-img0004.jpg "Western Blot: MT-ND5 Antibody [NBP3-05604] - Western blot analysis of extracts of various cell lines, using MT-ND5 antibody (NBP3-05604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: MT-ND5 Antibody [NBP3-05604] - Mouse kidney using MT-ND5 antibody (NBP3-05604) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/MT-ND5-Antibody-Immunohistochemistry-Paraffin-NBP3-05604-img0003.jpg)
![Immunohistochemistry-Paraffin: MT-ND5 Antibody [NBP3-05604] - Rat kidney using MT-ND5 antibody (NBP3-05604) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/MT-ND5-Antibody-Immunohistochemistry-Paraffin-NBP3-05604-img0001.jpg)
![Immunohistochemistry-Paraffin: MT-ND5 Antibody [NBP3-05604] - Human breast cancer using MT-ND5 antibody (NBP3-05604) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/MT-ND5-Antibody-Immunohistochemistry-Paraffin-NBP3-05604-img0002.jpg)
![Western Blot: Rab7a Antibody (10E4) [NBP2-60237] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab7A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysate Lane 3.: MCF7 cell lysate Lane 4.: PC3 cell lysateLane 5.: 293T cell lysateLane 6.: SK-OV-3 cell lysate](http://images.novusbio.com/fullsize/Rab7a-Antibody-10E4-Western-Blot-NBP2-60237-img0001.jpg "Western Blot: Rab7a Antibody (10E4) [NBP2-60237] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab7A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysate Lane 3.: MCF7 cell lysate Lane 4.: PC3 cell lysateLane 5.: 293T cell lysateLane 6.: SK-OV-3 cell lysate")
![Immunocytochemistry/Immunofluorescence: Rab7a Antibody (10E4) [NBP2-60237] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/Rab7a-Antibody-10E4-Immunocytochemistry-Immunofluorescence-NBP2-60237-img0002.jpg)
![Flow Cytometry: Rab7a Antibody (10E4) [NBP2-60237] - Analysis of RAB7A in HeLa cells. The cell was stained with at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotypecontrol (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Rab7a-Antibody-10E4-Flow-Cytometry-NBP2-60237-img0004.jpg)
![Western Blot: Huntingtin Antibody [NBP3-15492] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-15492-img0003.jpg "Western Blot: Huntingtin Antibody [NBP3-15492] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-15492] - Immunohistochemistry of paraffin-embedded rat brain using Huntingtin Rabbit pAb (NBP3-15492) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-15492-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody - Azide and BSA Free [Huntingtin] - Immunofluorescence analysis of U-251MG cells using Huntingtin Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15492_rabbit-huntingtin-pab-5220251734580.jpg)
![Western Blot: VGLUT2 Antibody [NBP2-94571] - Analysis of extracts of various cell lines, using VGLUT2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-Western-Blot-NBP2-94571-img0003.jpg "Western Blot: VGLUT2 Antibody [NBP2-94571] - Analysis of extracts of various cell lines, using VGLUT2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: VGLUT2 Antibody - Azide and BSA Free [NBP2-94571] - Immunofluorescence analysis of rat brain using VGLUT2 Rabbit pAb (A15177) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94571_rabbit-polyclonal-vglut2-antibody-52202519302916.jpg)
![Immunocytochemistry/ Immunofluorescence: VGLUT2 Antibody - Azide and BSA Free [NBP2-94571] - Immunofluorescence analysis of mouse brain using VGLUT2 Rabbit pAb (A15177) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94571_rabbit-polyclonal-vglut2-antibody-52202519294594.jpg)
![Western Blot: L1CAM Antibody [NBP3-03276] - Analysis of extracts of various cell lines, using L1CAM antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/L1CAM-Antibody-Western-Blot-NBP3-03276-img0002.jpg "Western Blot: L1CAM Antibody [NBP3-03276] - Analysis of extracts of various cell lines, using L1CAM antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: L1CAM Antibody - BSA Free [NBP3-03276] - Immunofluorescence analysis of SH-SY5Y cells using L1CAM Rabbit pAb (A8555) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03276_rabbit-polyclonal-l1cam-antibody-52202519294599.jpg)
![Immunocytochemistry/ Immunofluorescence: L1CAM Antibody - BSA Free [NBP3-03276] - Immunofluorescence analysis of HeLa cells using L1CAM Rabbit pAb (A8555) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03276_rabbit-polyclonal-l1cam-antibody-5220251929453.jpg)
![Immunocytochemistry/Immunofluorescence: L1CAM Antibody [NBP3-03276] - Analysis of Neuro-2a cells using [KO Validated] L1CAM Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/L1CAM-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03276-img0004.jpg)
![Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Western-Blot-NBP2-22411-img0003.jpg "Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.")
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0005.jpg)
![Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Western-Blot-NBP2-22401-img0003.jpg "Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.")
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Brevican Antibody (D) Composite.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Brevican Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0005.jpg)
![Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Western-Blot-NBP2-89836-img0002.jpg "Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836] - Analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated , stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Flow-Cytometry-NBP2-89836-img0004.jpg)
![Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate0.5 uL anti-CRABP2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 16 kDa Observed band size: 16 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Immunoprecipitation-NBP2-89836-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody (004) [NBP2-89836] - Staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89836 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89836_rabbit-monoclonal-crabp2-antibody-004-235202514331567.png)
![Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-288202414191534.jpg "Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Vimentin antibody (NBP3-33092) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33092) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-298202414304186.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-2920241192398.jpg)
![Western Blot: CREB3L2 Antibody [NBP2-87208] - Host: Rabbit. Target Name: CREB3L2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP2-87208-img0005.jpg "Western Blot: CREB3L2 Antibody [NBP2-87208] - Host: Rabbit. Target Name: CREB3L2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: CREB3L2 Antibody [NBP2-87208] - Human kidney](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-NBP2-87208-img0001.jpg)
![Western Blot: CREB3L2 Antibody [NBP2-87208] - WB Suggested Anti-CREB3L2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP2-87208-img0002.jpg)
![Western Blot: CREB3L2 Antibody [NBP2-87208] - Host: Rabbit. Target Name: CREB3L2. Sample Type: Human Fetal Brain. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP2-87208-img0003.jpg)
![Western Blot: CREB3L2 Antibody [NBP2-87208] - Host: Rabbit. Target Name: CREB3L2. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP2-87208-img0004.jpg)