| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03221-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95096-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35912-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61828-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59335-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-21805-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05182-0.025mg | |||
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP3-03221-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-03221] - Immunohistochemistry of paraffin-embedded human colon using [KO Validated] Vimentin Rabbit pAb (NBP3-03221) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP3-03221-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts of various cell lines, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Vimentin Antibody-Western Blot-NBP3-03221-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody - Azide and BSA Free [Vimentin] - Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03221_rabbit-polyclonal-vimentin-antibody-5220251628096.jpg)
![Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/RPE65-Antibody-Western-Blot-NBP2-95096-img0003.jpg "Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunohistochemistry: RPE65 Antibody [NBP2-95096] - Analysis of Mouse eye cells using RPE65 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-Immunohistochemistry-NBP2-95096-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519302965.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-5220251931373.jpg)
![Western Blot: RPE65 Antibody - BSA Free [NBP2-95096] - Western blot analysis of lysates from Mouse eye, using RPE65 Rabbit pAb (A9841) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519294553.jpg)
![Western Blot: Myelin PLP Antibody [NBP3-35912] - Western blot analysis of various lysates, using Myelin PLP Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35912_rabbit-myelin-plp-pab-9122024141812.jpg "Western Blot: Myelin PLP Antibody [NBP3-35912] - Western blot analysis of various lysates, using Myelin PLP Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunohistochemistry: Myelin PLP Antibody [NBP3-35912] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Myelin PLP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35912_rabbit-myelin-plp-pab-912202415121368.jpg)
![Immunocytochemistry/ Immunofluorescence: Myelin PLP Antibody [NBP3-35912] - Immunofluorescence analysis of paraffin-embedded mouse brain using Myelin PLP Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35912_rabbit-myelin-plp-pab-912202414201388.jpg)
![Immunocytochemistry/ Immunofluorescence: Myelin PLP Antibody [NBP3-35912] - Immunofluorescence analysis of paraffin-embedded rat brain using Myelin PLP Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35912_rabbit-myelin-plp-pab-912202415175210.jpg)
![Immunohistochemistry: Myelin PLP Antibody [NBP3-35912] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Myelin PLP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35912_rabbit-myelin-plp-pab-912202414154342.jpg)
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunohistochemistry-181202616152120.jpg "Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]")
![Western Blot:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-western-blot-18120261619287.jpg)
![Western Blot:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-western-blot-18120261615213.jpg)
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunohistochemistry-18120261618187.jpg)
![Immunofluorescence:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunofluorescence-181202616154110.jpg)
![Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Western-Blot-NBP2-61828-img0005.jpg "Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of Hela cells using GRM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunocytochemistry-Immunofluorescence-NBP2-61828-img0002.jpg)
![Immunohistochemistry: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of paraffin-embedded stomach cancer tissues using GRM5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunohistochemistry-NBP2-61828-img0004.jpg)
![Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against HEK293 (1) and GRM5 (AA: extra 458-580)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Western-Blot-NBP2-61828-img0006.jpg)
![Immunohistochemistry: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of paraffin-embedded bladder cancer tissues using GRM5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunohistochemistry-NBP2-61828-img0003.jpg)
![ELISA: mGluR5 Antibody (1A11D10) [NBP2-61828] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-ELISA-NBP2-61828-img0001.jpg)
![Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA-B R2 protein using Mouse Anti-GABA-B R2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-GABA-B R2 Monoclonal Antibody (NBP2-59335) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Western-Blot-NBP2-59335-img0005.jpg "Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA-B R2 protein using Mouse Anti-GABA-B R2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-GABA-B R2 Monoclonal Antibody (NBP2-59335) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody (NBP2-59335) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody (NBP2-59335) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-B Receptor 2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0007.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY CD38 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0003.jpg "Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY CD38 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.")
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0006.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Analysis of cell extracts (35 ug) from 5 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0002.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0004.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0005.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunofluorescence-NBP2-21805-img0002.jpg "Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Immunohistochemistry: N-Cadherin Antibody (GC-4) [NBP2-21805] - Frozen acetone fixed chicken cardiac sections stained with Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunohistochemistry-NBP2-21805-img0001.jpg)
![ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-32-BSA-Free-ELISA-NBP1-05182-img0001.jpg "ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.")
![Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NLS4229] - immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with GPR17.](http://images.novusbio.com/fullsize/GPR17-Antibody-Immunocytochemistry-Immunofluorescence-NLS4229-img0003.jpg "Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NLS4229] - immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with GPR17.")
![Immunohistochemistry-Paraffin: GPR17 Antibody [NLS4229] - Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR17-Antibody-Immunohistochemistry-Paraffin-NLS4229-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NLS4229] - immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/GPR17-Antibody-Immunocytochemistry-Immunofluorescence-NLS4229-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR17 Antibody [NLS4229] - IHC staining of human brain, neurons and glia.](http://images.novusbio.com/fullsize/GPR17-Antibody-Immunohistochemistry-Paraffin-NLS4229-img0001.jpg)
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-288202412442995.jpg "Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32850) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-298202413585526.jpg)
![Immunocytochemistry/ Immunofluorescence: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunocytochemistry analysis of HeLa cells labeling PRAS40 with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241192362.jpg)
![Flow Cytometry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Flow cytometric analysis of HeLa cells labeling PRAS40. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32850, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241637211.jpg)