| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80875-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41129-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12915-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12913-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33597-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38308-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41171-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76528-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72543 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22411-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42189-0.025mg | |||
![Immunohistochemistry-Paraffin: PSD-95 Antibody [NBP1-80875] - Analysis in human cerebral cortex and liver tissues using Anti-DLG4 antibody. Corresponding DLG4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PSD-95-Antibody-Immunohistochemistry-Paraffin-NBP1-80875-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PSD-95 Antibody [NBP1-80875] - Analysis in human cerebral cortex and liver tissues using Anti-DLG4 antibody. Corresponding DLG4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PSD-95 Antibody [NBP1-80875] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/PSD-95-Antibody-Immunohistochemistry-Paraffin-NBP1-80875-img0008.jpg)
![Immunohistochemistry-Paraffin: PSD-95 Antibody [NBP1-80875] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/PSD-95-Antibody-Immunohistochemistry-Paraffin-NBP1-80875-img0007.jpg)
![Western Blot: LRRTM2 Antibody [NBP2-41129] - Western blot analysis of LRRTM2 in SK-N-SH cell lysate with LRRTM2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/LRRTM2-Antibody-Western-Blot-NBP2-41129-img0001.jpg "Western Blot: LRRTM2 Antibody [NBP2-41129] - Western blot analysis of LRRTM2 in SK-N-SH cell lysate with LRRTM2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: LRRTM2 Antibody [NBP2-41129] - LRRTM2 staning is detected in neurites and cell bodies in mouse cortical neurons (dilution: 1:100 [red], DAPI: blue). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/LRRTM2-Antibody-Immunofluorescence-NBP2-41129-img0004.jpg)
![Immunohistochemistry: LRRTM2 Antibody - BSA Free [NBP2-41129] - Immunohistochemistry of LRRTM2 in human brain tissue with LRRTM2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41129_rabbit-polyclonal-lrrtm2-antibody-2711202420414523.jpg)
![Immunocytochemistry/ Immunofluorescence: LRRTM2 Antibody - BSA Free [NBP2-41129] - Immunofluorescence of LRRTM2 in human brain tissue with LRRTM2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41129_rabbit-polyclonal-lrrtm2-antibody-2711202420405534.jpg)
![Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK1-Antibody-N22-21-Western-Blot-NBP2-12915-img0004.jpg "Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK1 Antibody (N22/21) [NBP2-12915] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK1-Antibody-N22-21-Immunocytochemistry-Immunofluorescence-NBP2-12915-img0006.jpg)
![Immunohistochemistry: SHANK1 Antibody (N22/21) [NBP2-12915] - Immunohistochemistry analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining (upper layer aggregations of staining).](http://images.novusbio.com/fullsize/SHANK1-Antibody-N22-21-Immunohistochemistry-NBP2-12915-img0005.jpg)
![Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Western-Blot-NBP2-12913-img0003.jpg "Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK2 (pan) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0005.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Analysis in human cerebral cortex and pancreas tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Analysis in human cerebral cortex and pancreas tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human cerebellum, cerebral cortex, pancreas and testis using Anti-VGLUT1 antibody NBP2-33597 (A) shows similar protein distribution across tissues to independent antibody NBP2-39000 (B).](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0009.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0011.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0006.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0008.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0010.jpg)
![Immunohistochemistry-Paraffin: HOMER1 Antibody [NBP2-38308] - Staining of human colon.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Immunohistochemistry-Paraffin-NBP2-38308-img0007.jpg "Immunohistochemistry-Paraffin: HOMER1 Antibody [NBP2-38308] - Staining of human colon.")
![Immunohistochemistry-Paraffin: HOMER1 Antibody [NBP2-38308] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Immunohistochemistry-Paraffin-NBP2-38308-img0002.jpg)
![Immunohistochemistry-Paraffin: HOMER1 Antibody [NBP2-38308] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Immunohistochemistry-Paraffin-NBP2-38308-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HOMER1 Antibody [NBP2-38308] - Staining in human cerebral cortex and pancreas tissues using anti-HOMER1 antibody. Corresponding HOMER1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Immunohistochemistry-Paraffin-NBP2-38308-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HOMER1 Antibody [NBP2-38308] - Staining of human cerebral cortex, colon, heart muscle and pancreas using Anti-HOMER1 antibody NBP2-38308 (A) shows similar protein distribution across tissues to independent antibody NBP2-38309 (B).](http://images.novusbio.com/fullsize/HOMER1-Antibody-Immunohistochemistry-Paraffin-NBP2-38308-img0005.jpg)
![Immunohistochemistry-Paraffin: HOMER1 Antibody [NBP2-38308] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Immunohistochemistry-Paraffin-NBP2-38308-img0006.jpg)
![Western Blot: SHANK3 Antibody [NBP2-41171] - Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP2-41171-img0001.jpg "Western Blot: SHANK3 Antibody [NBP2-41171] - Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: SHANK3 Antibody - BSA Free [NBP2-41171] - Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41171_rabbit-polyclonal-shank3-antibody-2711202420333840.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody - BSA Free [NBP2-41171] - Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41171_rabbit-polyclonal-shank3-antibody-271120241926181.jpg)
![Western Blot: LRRTM1 Antibody [NBP1-76528] - Mouse brain tissue lysate with LRRTM1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/LRRTM1-Antibody-Western-Blot-NBP1-76528-img0001.jpg "Western Blot: LRRTM1 Antibody [NBP1-76528] - Mouse brain tissue lysate with LRRTM1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: LRRTM1 Antibody [NBP1-76528] - Immunofluorescence of LRRTM1 in human brain tissue with LRRTM1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/LRRTM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76528-img0002.jpg)
![Immunohistochemistry: LRRTM1 Antibody - BSA Free [NBP1-76528] - Immunohistochemistry of LRRTM1 in human brain tissue with LRRTM1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76528_rabbit-polyclonal-lrrtm1-antibody-2711202419293818.jpg)
![Western Blot: LRRTM1 Antibody (5H9) [NBP2-45675] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRTM1.](http://images.novusbio.com/fullsize/LRRTM1-Antibody-5H9-Western-Blot-NBP2-45675-img0004.jpg "Western Blot: LRRTM1 Antibody (5H9) [NBP2-45675] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRTM1.")
![Immunohistochemistry: LRRTM1 Antibody (5H9) [NBP2-45675] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/LRRTM1-Antibody-5H9-Immunohistochemistry-NBP2-45675-img0003.jpg)
![Immunohistochemistry: LRRTM1 Antibody (5H9) [NBP2-45675] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/LRRTM1-Antibody-5H9-Immunohistochemistry-NBP2-45675-img0001.jpg)
![Immunohistochemistry: LRRTM1 Antibody (5H9) [NBP2-45675] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/LRRTM1-Antibody-5H9-Immunohistochemistry-NBP2-45675-img0002.jpg)
![Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Western-Blot-NBP2-22411-img0003.jpg "Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.")
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0005.jpg)
![Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Western-Blot-NBP2-42189-img0003.jpg "Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1/SHANK3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0002.jpg)
![Western Blot: PTBP2 Antibody (JE66-55) [NBP3-32871] - Western blot analysis of PTBP2 on different lysates with Rabbit anti-PTBP2 antibody (NBP3-32871) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate (15 ug/Lane) Lane 2: Mouse testis tissue lysate (30 ug/Lane) Lane 3: Rat testis tissue lysate (30 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32871) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32871_rabbit-ptbp2-mab-je66-55-288202412442959.jpg "Western Blot: PTBP2 Antibody (JE66-55) [NBP3-32871] - Western blot analysis of PTBP2 on different lysates with Rabbit anti-PTBP2 antibody (NBP3-32871) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate (15 ug/Lane) Lane 2: Mouse testis tissue lysate (30 ug/Lane) Lane 3: Rat testis tissue lysate (30 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32871) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTBP2 Antibody (JE66-55) [NBP3-32871] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PTBP2 antibody (NBP3-32871) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32871) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32871_rabbit-ptbp2-mab-je66-55-29820241492646.jpg)
![Flow Cytometry: PTBP2 Antibody (JE66-55) [NBP3-32871] - Flow cytometric analysis of HeLa cells labeling PTBP2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32871, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32871_rabbit-ptbp2-mab-je66-55-29202416453283.jpg)