| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44974 | |||
| Azide and BSA Free | NBP2-34751-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48002-0.1mg | |||
| IHC-Prediluted | NBP2-48186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92715SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95252-0.02ml | |||
![Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Western-Blot-NBP2-32865-img0005.jpg "Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (SPM252) [NBP2-32865] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunocytochemistry-Immunofluorescence-NBP2-32865-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0006.jpg)
![Flow Cytometry: HSP27 Antibody (SPM252) [NBP2-32865] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (SPM252).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Flow-Cytometry-NBP2-32865-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (20X) stained with HSP27 MAb (SPM252)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (10X) stained with HSP27 MAb (SPM252))](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0001.jpg)
![Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.](http://images.novusbio.com/fullsize/HSP27-Antibody-Western-Blot-NB120-5579-img0003.jpg "Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0008.jpg)
![Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Western-Blot-NBP3-15677-img0007.jpg "Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded mouse brain using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0003.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded rat ovary using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0001.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded human esophageal using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0002.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315540.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of HeLa cells using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517352713.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunoprecipitation of TDP-43/TARDBP from 500 ug extracts of K-562 cells was performed using 2 ug of TDP-43/TARDBP Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X non-reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TDP-43/TARDBP Rabbit mAb at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-5220251734597.jpg)
![Chromatin Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Chromatin immunoprecipitation analysis of extracts of K562 cells, using TDP-43/TARDBP Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517344289.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of paraffin-embedded Mouse brain tissue using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517363742.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517361227.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Human colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315511.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517311072.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat liver using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517304973.jpg)
![Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Western-Blot-NBP2-75440-img0009.jpg "Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney")
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0004.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0008.jpg)
![Flow Cytometry: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Flow-Cytometry-NBP2-75440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0003.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0005.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0006.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0007.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: KCNJ10 Antibody [NBP1-20149] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Western-Blot-NBP1-20149-img0013.jpg "Western Blot: KCNJ10 Antibody [NBP1-20149] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0012.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0002.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0005.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of mouse kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer from following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0006.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0007.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0009.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat heart. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0010.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0011.jpg)
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)
![Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Western-Blot-NBP2-44975-img0006.jpg "Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (HSPB1/774) [NBP2-44975] - ICC/IF analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunofluorescence-NBP2-44975-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0002.jpg)
![Flow Cytometry: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Flow-Cytometry-NBP2-44975-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0001.jpg)
![Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Western blot shows lysates of 293T human embryonic kidney parental cell line and PARK7 knockout (KO) 293T cell line. PVDF membrane was probed with Mouse Anti-Human PARK7 Monoclonal Antibody (Catalog # NBP1-92715) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PARK7 at approximately 23 kDa (as indicated) in the parental 293T cell line, but is not detectable in the knockout 293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Knockout-Validated-NBP1-92715-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Western blot shows lysates of 293T human embryonic kidney parental cell line and PARK7 knockout (KO) 293T cell line. PVDF membrane was probed with Mouse Anti-Human PARK7 Monoclonal Antibody (Catalog # NBP1-92715) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PARK7 at approximately 23 kDa (as indicated) in the parental 293T cell line, but is not detectable in the knockout 293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Analysis of whole brain and cell lysates using mouse mAb against DJ1/Park7, NBP1-92715, dilution 1:5,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The DJ1 antibody detects protein with apparent molecular weight of 21kDa but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken pAb to vimentin, dilution 1;5,000 in red, revealing a single band at about 50kDa present in all lanes, though at much lower levels in the tissue lysates.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Western-Blot-NBP1-92715-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Park7(DJ-1) Antibody (4H4) [NBP1-92715] - HeLa cells stained with NBP1-92715 (green), chicken antibody to Vimentin (NB300-223, red) and DNA (blue). NBP1-92715 reveals strong cytoplasmic staining for Park7(DJ-1).](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Immunocytochemistry-Immunofluorescence-NBP1-92715-img0003.jpg)
![Simple Western: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Lane view shows a specific band for Park7 (DJ-1) in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Simple-Western-NBP1-92715-img0004.jpg)
![Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Western-Blot-NBP2-34621-img0002.jpg "Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34621-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34621-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0005.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Western-Blot-NBP2-95252-img0008.jpg "Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of U2OS cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251639688.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat pancreas using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0007.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of 293T cells, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/IRE1 alpha Antibody-Western Blot-NBP2-95252-img0009.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Human breast cancer using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0004.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Mouse kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0005.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of PC-12 cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-52202516334127.jpg)
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of NIH/3T3 cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251640267.jpg)
![Immunohistochemistry: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251633413.jpg)