| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59374-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87709-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94268-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94084-0.02ml | |||
![Western Blot: LEDGF Antibody (3H1) [H00011168-M01] - PSIP1 monoclonal antibody (M01), clone 3H1. Analysis of PSIP1 expression in human liver.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Western-Blot-H00011168-M01-img0007.jpg "Western Blot: LEDGF Antibody (3H1) [H00011168-M01] - PSIP1 monoclonal antibody (M01), clone 3H1. Analysis of PSIP1 expression in human liver.")
![Immunohistochemistry-Paraffin: LEDGF Antibody (3H1) [H00011168-M01] - Analysis of monoclonal antibody to PSIP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Immunohistochemistry-Paraffin-H00011168-M01-img0005.jpg)
![Sandwich ELISA: LEDGF Antibody (3H1) [H00011168-M01] - Detection limit for recombinant GST tagged PSIP1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Sandwich-ELISA-H00011168-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0004.jpg)
![Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-278202415295538.jpg "Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Kir2.1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32505, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-298202414333623.jpg)
![Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (JE54-56) [NBP3-32505] - ICC staining of Kir2.1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32505, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-29202410484869.jpg)
![Western Blot: Caspr1 Antibody [NBP3-13184] - Various tissue extracts (75 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Caspr1 antibody (NBP3-13184) diluted at 1:1000.](http://images.novusbio.com/fullsize/Caspr1-Antibody-Western-Blot-NBP3-13184-img0001.jpg "Western Blot: Caspr1 Antibody [NBP3-13184] - Various tissue extracts (75 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Caspr1 antibody (NBP3-13184) diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Caspr1 Antibody [NBP3-13184] - Caspr1 antibody detects Caspr1 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Caspr1 antibody (NBP3-13184) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Caspr1-Antibody-Immunohistochemistry-Paraffin-NBP3-13184-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspr1 Antibody [NBP3-13184] - Caspr1 antibody detects Caspr1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Caspr1 antibody (NBP3-13184) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Caspr1-Antibody-Immunohistochemistry-Paraffin-NBP3-13184-img0002.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0007.jpg "Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.")
![Immunohistochemistry: Kir2.1 Antibody [NBP1-87709] - Staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-NBP1-87709-img0004.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0005.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0006.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0008.jpg)
![Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-278202416285253.jpg "Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32486) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-298202414304155.jpg)
![Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunocytochemistry analysis of NCCIT cells labeling Jumonji/JARID2 with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-29202410484844.jpg)
![Immunoprecipitation: RDH10 Antibody - BSA Free [NBP3-46767] - IP Result of anti-RDH10 (IP:, 3ug; Detection: 1:700) with mouse liver tissue lysate 4000ug.](http://images.novusbio.com/fullsize/nbp3-46767_rabbit-rdh10-pab-bsa-free-284202512315728.jpg "Immunoprecipitation: RDH10 Antibody - BSA Free [NBP3-46767] - IP Result of anti-RDH10 (IP:, 3ug; Detection: 1:700) with mouse liver tissue lysate 4000ug.")
![Western Blot: RDH10 Antibody - BSA Free [NBP3-46767] - Human kidney tissue were subjected to SDS PAGE followed by western blot with (RDH10 antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-46767_rabbit-rdh10-pab-bsa-free-28420251235683.jpg)
![Immunohistochemistry: RDH10 Antibody - BSA Free [NBP3-46767] - Immunohistochemistry of paraffin-embedded human lung cancer using (RDH10 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-46767_rabbit-rdh10-pab-bsa-free-28420251233178.jpg)
![Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 expression in transfected 293T cell line by OTX1 monoclonal antibody (M01), clone 1F2.Lane 1: OTX1 transfected lysate(37.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Otx1-Antibody-1F2-Western-Blot-H00005013-M01-img0008.jpg "Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 expression in transfected 293T cell line by OTX1 monoclonal antibody (M01), clone 1F2.Lane 1: OTX1 transfected lysate(37.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of monoclonal antibody to OTX1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Otx1-Antibody-1F2-Immunocytochemistry-Immunofluorescence-H00005013-M01-img0005.jpg)
![Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 over-expressed 293 cell line, cotransfected with OTX1 Validated Chimera RNAi ( Cat # H00005013-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with OTX1 monoclonal antibody (M01), clone 1F2 (Cat # H00005013-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Otx1-Antibody-1F2-Western-Blot-H00005013-M01-img0006.jpg)
![ELISA: Otx1 Antibody (1F2) [H00005013-M01] - Detection limit for recombinant GST tagged OTX1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Otx1-Antibody-1F2-ELISA-H00005013-M01-img0009.jpg)
![Western Blot: NFkB p100/p52 Antibody [NBP2-94268] - Western blot analysis of extracts of various cell lines, using NFkB p100/p52 antibody (NBP2-94268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/NFkB p100-p52 Antibody-Western Blot-NBP2-94268-img0001.jpg "Western Blot: NFkB p100/p52 Antibody [NBP2-94268] - Western blot analysis of extracts of various cell lines, using NFkB p100/p52 antibody (NBP2-94268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: OTP Antibody [NBP2-94084] - Analysis of extracts of various cell lines, using OTP at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/OTP-Antibody-Western-Blot-NBP2-94084-img0002.jpg "Western Blot: OTP Antibody [NBP2-94084] - Analysis of extracts of various cell lines, using OTP at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: OTP Antibody [NBP2-94084] - Paraffin-embedded human lung cancer using OTP .](http://images.novusbio.com/fullsize/OTP-Antibody-Immunohistochemistry-Paraffin-NBP2-94084-img0001.jpg)
![Western Blot: NDUFS3 Antibody (2R6F5) [NBP3-16287] - Western blot analysis of extracts of various cell lines, using NDUFS3 Rabbit mAb (NBP3-16287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/NDUFS3-Antibody-2R6F5-Western-Blot-NBP3-16287-img0002.jpg "Western Blot: NDUFS3 Antibody (2R6F5) [NBP3-16287] - Western blot analysis of extracts of various cell lines, using NDUFS3 Rabbit mAb (NBP3-16287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: NDUFS3 Antibody (2R6F5) [NBP3-16287] - Immunohistochemistry of paraffin-embedded rat liver using NDUFS3 Rabbit mAb (NBP3-16287) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NDUFS3-Antibody-2R6F5-Immunohistochemistry-Paraffin-NBP3-16287-img0001.jpg)
![Western Blot: LEDGF Antibody (JE75-02) [NBP3-32859] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: Huh7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32859_rabbit-ledgf-mab-je75-02-288202412461663.jpg "Western Blot: LEDGF Antibody (JE75-02) [NBP3-32859] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: Huh7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEDGF Antibody (JE75-02) [NBP3-32859] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32859) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32859_rabbit-ledgf-mab-je75-02-29820241420993.jpg)