| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22590-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04802-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03492-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-30792-0.1mg | |||
![Western Blot: PPP2R5D Antibody (SR2044) [NBP3-22590] - Western blot analysis using NBP3-22590 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22590_rabbit-ppp2r5d-mab-sr2044-57202317215114.jpg "Western Blot: PPP2R5D Antibody (SR2044) [NBP3-22590] - Western blot analysis using NBP3-22590 in HeLa cell lysate.")
![Western Blot: KCNJ1 Antibody [NBP3-04802] - Analysis of extracts of mouse kidney, using KCNJ1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .](http://images.novusbio.com/fullsize/KCNJ1-Antibody-Western-Blot-NBP3-04802-img0003.jpg "Western Blot: KCNJ1 Antibody [NBP3-04802] - Analysis of extracts of mouse kidney, using KCNJ1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .")
![Immunohistochemistry-Paraffin: KCNJ1 Antibody [NBP3-04802] - Paraffin-embedded rat pancreas using KCNJ1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KCNJ1-Antibody-Immunohistochemistry-Paraffin-NBP3-04802-img0002.jpg)
![Immunohistochemistry-Paraffin: KCNJ1 Antibody [NBP3-04802] - Paraffin-embedded rat testis using KCNJ1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KCNJ1-Antibody-Immunohistochemistry-Paraffin-NBP3-04802-img0001.jpg)
![Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-288202412442968.jpg "Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-2920241192344.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32846, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-29202416372164.jpg)
![Western Blot: PPP2R5D Antibody (1A3) [H00005528-M21] - Analysis of PPP2R5D expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-1A3-Western-Blot-H00005528-M21-img0004.jpg "Western Blot: PPP2R5D Antibody (1A3) [H00005528-M21] - Analysis of PPP2R5D expression in Jurkat (Cat # L017V1).")
![Immunocytochemistry/Immunofluorescence: PPP2R5D Antibody (1A3) [H00005528-M21] - Analysis of monoclonal antibody to PPP2R5D on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-1A3-Immunocytochemistry-Immunofluorescence-H00005528-M21-img0002.jpg)
![Sandwich ELISA: PPP2R5D Antibody (1A3) [H00005528-M21] - Detection limit for recombinant GST tagged PPP2R5D is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-1A3-Sandwich-ELISA-H00005528-M21-img0003.jpg)
![Western Blot: SLC12A3 Antibody [NBP1-59699] - ACHN cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Western-Blot-NBP1-59699-img0002.jpg "Western Blot: SLC12A3 Antibody [NBP1-59699] - ACHN cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: PPP2R5D Antibody [NB100-57536] - Detection of Human PPP2R5D on HeLa whole cell lysate using NB100-57536. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies NB100-57537 and NB100-57538.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NB100-57536-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PPP2R5D Antibody [NB100-57536] - Detection of Human PPP2R5D on HeLa whole cell lysate using NB100-57536. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies NB100-57537 and NB100-57538.")
![SDS-Page: Recombinant Human CNPase Protein [H00001267-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001267-P01-1.jpg "SDS-Page: Recombinant Human CNPase Protein [H00001267-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: KCNJ1 Antibody [NBP2-87660] - Lanes: Lane 1: 30ug mouse renal epithelial lysate. Lane 2: 30ug mouse renal epithelial lysate. Lane 3: 30ug mouse renal epithelial lysate. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:2500. Gene Name: KCNJ1. Submitted by: Dr. JUNMING FAN,ECU.](http://images.novusbio.com/fullsize/KCNJ1-Antibody-Western-Blot-NBP2-87660-img0002.jpg "Western Blot: KCNJ1 Antibody [NBP2-87660] - Lanes: Lane 1: 30ug mouse renal epithelial lysate. Lane 2: 30ug mouse renal epithelial lysate. Lane 3: 30ug mouse renal epithelial lysate. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:2500. Gene Name: KCNJ1. Submitted by: Dr. JUNMING FAN,ECU.")
![Western Blot: KCNJ1 Antibody [NBP2-87660] - WB Suggested Anti-KCNJ1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/KCNJ1-Antibody-Western-Blot-NBP2-87660-img0001.jpg)
![Western Blot: PPP2R5D Antibody [NB100-57538] - Detection of Human and Mouse PPP2R5D on HeLa whole cell lysate using NB100-57538. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies NB100-57536 and NB100-57537.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NB100-57538-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PPP2R5D Antibody [NB100-57538] - Detection of Human and Mouse PPP2R5D on HeLa whole cell lysate using NB100-57538. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies NB100-57536 and NB100-57537.")
![Western Blot: KCNJ1 Antibody [NBP1-36965] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/KCNJ1-Antibody-Western-Blot-NBP1-36965-img0002.jpg "Western Blot: KCNJ1 Antibody [NBP1-36965] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: CNPase Antibody [NBP3-03492] - Analysis of extracts from normal (control) and CNP knockout (KO) HeLa cells, using CNPase antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/CNPase-Antibody-Knockout-Validated-NBP3-03492-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CNPase Antibody [NBP3-03492] - Analysis of extracts from normal (control) and CNP knockout (KO) HeLa cells, using CNPase antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")