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![Western Blot: TOMM40 Antibody [NBP3-13290] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TOMM40 antibody (NBP3-13290) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM40-Antibody-Western-Blot-NBP3-13290-img0001.jpg "Western Blot: TOMM40 Antibody [NBP3-13290] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TOMM40 antibody (NBP3-13290) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Semaphorin 7A Antibody (HL1826) - Azide and BSA Free [NBP3-25677] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA7A antibody [HL1826] (NBP3-25677) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25677_rabbit-semaphorin-7a-mab-hl1826-azide-and-bsa-free-21202494146.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Semaphorin 7A Antibody (HL1826) - Azide and BSA Free [NBP3-25677] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA7A antibody [HL1826] (NBP3-25677) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: CLN5 Antibody [NBP3-12971] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CLN5 antibody (NBP3-12971) diluted at 1:1000.](http://images.novusbio.com/fullsize/CLN5-Antibody-Western-Blot-NBP3-12971-img0001.jpg "Western Blot: CLN5 Antibody [NBP3-12971] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CLN5 antibody (NBP3-12971) diluted at 1:1000.")
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HFE antibody [HL2155] (NBP3-48673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48673_rabbit-hfe-mab-hl2155-western-blot-1411202518243315.jpg "<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HFE antibody [HL2155] (NBP3-48673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Untreated (–) and treated (+) SH-SY5Y whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Tau (pSer235) antibody (HL3899) diluted at 1:1000 and Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45162_rabbit-tau-p-ser235-mab-hl3899-azide-and-bsa-free-western-blot-2942025155710..jpg "<b>Biological Strategies Validation. </b>Untreated (–) and treated (+) SH-SY5Y whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Tau (pSer235) antibody (HL3899) diluted at 1:1000 and Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NBP3-13299] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II alpha (phospho Thr286) antibody (NBP3-13299) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-[p-Thr286]-Antibody-Western-Blot-NBP3-13299-img0001.jpg "Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NBP3-13299] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II alpha (phospho Thr286) antibody (NBP3-13299) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: IGF-I R/IGF1R [p Tyr1146] Antibody [NBP3-13236] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (NBP3-13236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-[p-Tyr1146]-Antibody-Western-Blot-NBP3-13236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IGF-I R/IGF1R [p Tyr1146] Antibody [NBP3-13236] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (NBP3-13236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Pet1 Antibody (HL2045) - Azide and BSA Free [NBP3-25597] - Pet1 antibody [HL2045] detects Pet1 protein at nucleus by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pet1 stained by Pet1 antibody [HL2045] (NBP3-25597) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25597_rabbit-pet1-mab-hl2045-azide-and-bsa-free-2120249362788.jpg "Immunocytochemistry/Immunofluorescence: Pet1 Antibody (HL2045) - Azide and BSA Free [NBP3-25597] - Pet1 antibody [HL2045] detects Pet1 protein at nucleus by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pet1 stained by Pet1 antibody [HL2045] (NBP3-25597) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .")
![Western Blot: Pet1 Antibody (HL2043) - Azide and BSA Free [NBP3-25596] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Pet1 antibody [HL2043] (NBP3-25596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25596_rabbit-pet1-mab-hl2043-azide-and-bsa-free-2120249422660.jpg "Western Blot: Pet1 Antibody (HL2043) - Azide and BSA Free [NBP3-25596] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Pet1 antibody [HL2043] (NBP3-25596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-[p-Tyr950]-Antibody-Western-Blot-NBP3-13235-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![SDS-Page: Recombinant Human Recoverin Protein [NBC1-18499] - Recoverin, 23 kDa (200 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Recombinant-Human-Recoverin-Protein-SDS-Page-NBC1-18499-img0002.jpg "SDS-Page: Recombinant Human Recoverin Protein [NBC1-18499] - Recoverin, 23 kDa (200 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
![SDS-Page: Recombinant Human Recoverin Protein [NBC1-18499] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-Recoverin-Protein-SDS-Page-NBC1-18499-img0003.jpg)
![Western Blot: SERF2 Overexpression Lysate (Adult Normal) [NBL1-15825] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SERF2.](http://images.novusbio.com/fullsize/SERF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15825-img0002.jpg "Western Blot: SERF2 Overexpression Lysate (Adult Normal) [NBL1-15825] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SERF2.")