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![Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunofluorescence analysis of paraffin-embedded AD mouse brain tissue labeling Abeta 42 with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32078, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32078_rabbit-abeta-42-mab-psh02-83-298202414161849.jpg "Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunofluorescence analysis of paraffin-embedded AD mouse brain tissue labeling Abeta 42 with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32078, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunohistochemical analysis of paraffin-embedded mouse model of Alzheimer's brain tissue with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32078) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32078_rabbit-abeta-42-mab-psh02-83-298202413585551.jpg)
![Western Blot: VGF Antibody (6#) [NBP3-41614] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: U87MG cell lysate Primary Ab: 3ug/ml NBP3-41614; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41614_mouse-vgf-mab-6-61220242116219.jpg "Western Blot: VGF Antibody (6#) [NBP3-41614] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: U87MG cell lysate Primary Ab: 3ug/ml NBP3-41614; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: VGF Antibody (6#) [NBP3-41614] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: U87MG cell lysate Primary Ab: 3ug/ml NBP3-41614; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41614_mouse-vgf-mab-6-612202421243289.jpg)
![Western Blot: Tryptophan rich protein Antibody [H00007485-B02P] - Analysis of WRB expression in rat brain.](http://images.novusbio.com/fullsize/Tryptophan-rich-protein-Antibody-Western-Blot-H00007485-B02P-img0001.jpg "Western Blot: Tryptophan rich protein Antibody [H00007485-B02P] - Analysis of WRB expression in rat brain.")
![Western Blot: Tryptophan rich protein Antibody [H00007485-B02P] - Analysis of WRB expression in transfected 293T cell line by WRB polyclonal antibody. Lane 1: WRB transfected lysate(19.80 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Tryptophan-rich-protein-Antibody-Western-Blot-H00007485-B02P-img0002.jpg)
![Immunoprecipitation:Nardilysin AntibodyNBP3-30543] -Detection of human NRD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NRD1 antibody NBP3-30543 (lot NBP3-30543-1) used for IP at 6 µg per reaction. NRD1 was also immunoprecipitated by rabbit anti-NRD1 antibody BL19671. For blotting immunoprecipitated NRD1, NBP3-30543 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30543_rabbit-nardilysin-pab-3012202412392179.jpg "Immunoprecipitation:Nardilysin AntibodyNBP3-30543] -Detection of human NRD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NRD1 antibody NBP3-30543 (lot NBP3-30543-1) used for IP at 6 µg per reaction. NRD1 was also immunoprecipitated by rabbit anti-NRD1 antibody BL19671. For blotting immunoprecipitated NRD1, NBP3-30543 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: SYPL1 Antibody [NBP1-76316] - Human brain tissue lysate with SYPL1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/SYPL1-Antibody-Western-Blot-NBP1-76316-img0001.jpg "Western Blot: SYPL1 Antibody [NBP1-76316] - Human brain tissue lysate with SYPL1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Western Blot: SNAP23 Antibody [NBP1-52065] - (0.2ug/ml) Analysis of PBMC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP1-52065-img0001.jpg "Western Blot: SNAP23 Antibody [NBP1-52065] - (0.2ug/ml) Analysis of PBMC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: SynGAP Antibody [NBP2-27558] - NBP2-27558 (2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Western-Blot-NBP2-27558-img0001.jpg "Western Blot: SynGAP Antibody [NBP2-27558] - NBP2-27558 (2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Chromogranin C Antibody [NBP2-42511] - Analysis of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Chromogranin-C-Antibody-Western-Blot-NBP2-42511-img0001.jpg "Western Blot: Chromogranin C Antibody [NBP2-42511] - Analysis of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376] - Western blot analysis of extracts of various cell lines, using GABARAP Rabbit mAb (NBP3-16376) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GABARAP-Antibody-5T6W5-Western-Blot-NBP3-16376-img0001.jpg "Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376] - Western blot analysis of extracts of various cell lines, using GABARAP Rabbit mAb (NBP3-16376) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: SLC31A1/CTR1 Antibody (4C10C5) [NBP3-15304] - Western blot analysis of extracts of various cell lines, using SLC31A1/CTR1 antibody (NBP3-15304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-4C10C5-Western-Blot-NBP3-15304-img0001.jpg "Western Blot: SLC31A1/CTR1 Antibody (4C10C5) [NBP3-15304] - Western blot analysis of extracts of various cell lines, using SLC31A1/CTR1 antibody (NBP3-15304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")