| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95107-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13538-0.05mg | |||
| BSA Free | NB120-13538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73178 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65204-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93572-0.02ml | |||
![Western Blot: PAK1 Antibody [NBP2-95107] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP2-95107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PAK1 Antibody-Western Blot-NBP2-95107-img0001.jpg "Western Blot: PAK1 Antibody [NBP2-95107] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP2-95107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: PAK1 Antibody - Azide and BSA Free [PAK1] - Western blot analysis of various lysates using PAK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp2-95107_rabbit-polyclonal-pak1-antibody-5220251638053.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538] - Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-h-151-Western-Blot-NB120-13538-img0004.jpg "Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538] - Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).")
![Immunofluorescence: SRCAP Antibody [NBP2-76468] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunofluorescence-NBP2-76468-img0001.jpg "Immunofluorescence: SRCAP Antibody [NBP2-76468] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Melanocortin-1-R-MC1R-Antibody-Immunohistochemistry-Paraffin-NLS1040-img0002.jpg "Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Melanocortin-1-R-MC1R-Antibody-Immunohistochemistry-Paraffin-NLS1040-img0001.jpg)
![Western Blot: UBL5 Antibody [NB600-470] - Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using a 1:500 dilution of IgG fraction of Rabbit-anti-Hub1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4 C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Hub1 is highly specific and does not cross react with other UBLs.](http://images.novusbio.com/fullsize/UBL5-Antibody-Western-Blot-NB600-470-img0003.jpg "Western Blot: UBL5 Antibody [NB600-470] - Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using a 1:500 dilution of IgG fraction of Rabbit-anti-Hub1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4 C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Hub1 is highly specific and does not cross react with other UBLs.")
![Western Blot: UBL5 Antibody [NB600-470] - Anti-Hub1 raised from recombinant yeast Hub1. All UBLs possess limited homology to Ubiquitin & to each other; hence it is important to know degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins after reaction with 1:1,000 dilution of anti-GFP followed by reaction with 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using 1:500 dilution of Rabbit-anti-Hub1 (Yeast) followed by reaction with 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primaries were diluted in TTBS buffer with 5% non-fat milk & incubated with membranes overnight at 4C. Yeast lysate proteins separated by SDS-PAGE using a 15% gel. Data indicates that anti-Hub1 is highly specific & does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche).](http://images.novusbio.com/fullsize/UBL5-Antibody-Western-Blot-NB600-470-img0001.jpg)
![Western Blot: CLN3 Antibody [H00001201-D01P] - Analysis of CLN3 expression in mouse kidney.](http://images.novusbio.com/fullsize/CLN3-Antibody-Western-Blot-H00001201-D01P-img0003.jpg "Western Blot: CLN3 Antibody [H00001201-D01P] - Analysis of CLN3 expression in mouse kidney.")
![Western Blot: CLN3 Antibody [H00001201-D01P] - Analysis of CLN3 expression in transfected 293T cell line by CLN3 polyclonal antibody.Lane 1: CLN3 transfected lysate(47.60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CLN3-Antibody-Western-Blot-H00001201-D01P-img0004.jpg)
![Immunoprecipitation:Nardilysin AntibodyNBP3-30543] -Detection of human NRD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NRD1 antibody NBP3-30543 (lot NBP3-30543-1) used for IP at 6 µg per reaction. NRD1 was also immunoprecipitated by rabbit anti-NRD1 antibody BL19671. For blotting immunoprecipitated NRD1, NBP3-30543 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30543_rabbit-nardilysin-pab-3012202412392179.jpg "Immunoprecipitation:Nardilysin AntibodyNBP3-30543] -Detection of human NRD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NRD1 antibody NBP3-30543 (lot NBP3-30543-1) used for IP at 6 µg per reaction. NRD1 was also immunoprecipitated by rabbit anti-NRD1 antibody BL19671. For blotting immunoprecipitated NRD1, NBP3-30543 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Oxytocin/Neurophysin I Antibody (OTI5G4) [NBP2-46308] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Oxytocin.](http://images.novusbio.com/fullsize/Oxytocin-Antibody-5G4-Western-Blot-NBP2-46308-img0001.jpg "Western Blot: Oxytocin/Neurophysin I Antibody (OTI5G4) [NBP2-46308] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Oxytocin.")
![Immunohistochemistry-Paraffin: Orexin A/Hypocretin-1 Antibody [NB100-65204] - Staining of paraffin embedded human hypothalamus with Sheep anti Human Orexin A.](http://images.novusbio.com/fullsize/Orexin-A-Hypocretin-1-Antibody-Immunohistochemistry-Paraffin-NB100-65204-img0003.jpg "Immunohistochemistry-Paraffin: Orexin A/Hypocretin-1 Antibody [NB100-65204] - Staining of paraffin embedded human hypothalamus with Sheep anti Human Orexin A.")
![Western Blot: REEP1 Antibody [NBP1-69643] - This Anti-REEP1 antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize/REEP1-Antibody-Western-Blot-NBP1-69643-img0001.jpg "Western Blot: REEP1 Antibody [NBP1-69643] - This Anti-REEP1 antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml.")
![Western Blot: REEP1 Antibody [NBP1-69643] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/REEP1-Antibody-Western-Blot-NBP1-69643-img0002.jpg)
![Western Blot: SIM1 Antibody [NBP1-97773] - NBP1-97773 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SIM1-Antibody-Western-Blot-NBP1-97773-img0004.jpg "Western Blot: SIM1 Antibody [NBP1-97773] - NBP1-97773 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: MT-ND4 Antibody [NBP2-93572] - Analysis of extracts of various cell lines, using MT-ND4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Western-Blot-NBP2-93572-img0001.jpg "Western Blot: MT-ND4 Antibody [NBP2-93572] - Analysis of extracts of various cell lines, using MT-ND4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.")