| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86985-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83162-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90250-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77323-0.025mg | |||
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-86985_rabbit-polyclonal-syntaxin-12-antibody-30420251393622.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249383145.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-212024943559.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249403847.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249371547.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249415950.jpg)
![Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-immunohistochemistry-paraffin-14112025183373.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0009.jpg "Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of monoclonal antibody to SNAP23 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunohistochemistry-Paraffin-H00008773-M01-img0006.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - SNAP23 expression in Raw 264.7.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0001.jpg)
![Immunoprecipitation: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 transfected lysate using anti-SNAP23 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SNAP23 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunoprecipitation-H00008773-M01-img0007.jpg)
![Sandwich ELISA: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Detection limit for recombinant GST tagged SNAP23 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Sandwich-ELISA-H00008773-M01-img0008.jpg)
![Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP1-83162-img0006.jpg "Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.")
![Immunocytochemistry/Immunofluorescence: SNAP23 Antibody [NBP1-83162] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83162-img0008.jpg)
![Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-83162] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunohistochemistry-Paraffin-NBP1-83162-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Piccolo Antibody [NBP1-90250] - Staining of human cell line U-251 MG shows localization to nuclear speckles & plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Piccolo-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90250-img0004.jpg "Immunocytochemistry/Immunofluorescence: Piccolo Antibody [NBP1-90250] - Staining of human cell line U-251 MG shows localization to nuclear speckles & plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Piccolo-Antibody-Immunohistochemistry-Paraffin-NBP1-90250-img0006.jpg)
![Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250] - Staining of human testis shows weak cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Piccolo-Antibody-Immunohistochemistry-Paraffin-NBP1-90250-img0008.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-212024941483.jpg "Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-21202494082.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249383198.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249415927.jpg)
![Synaptotagmin 1 antibody [HL1626] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518381714.jpg)
![Western Blot: SNAP23 Antibody [NBP2-20434] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP23 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP2-20434-img0004.jpg "Western Blot: SNAP23 Antibody [NBP2-20434] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP23 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SNAP23 Antibody [NBP2-20434] - SNAP23 antibody detects SNAP23 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP23 protein stained by SNAP23 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20434-img0005.jpg)
![Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP2-20434] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunohistochemistry-Paraffin-NBP2-20434-img0002.jpg)
![Western Blot: SNAP23 Antibody [NBP2-20434] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP2-20434-img0001.jpg)
![Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - STX1A monoclonal antibody (M02), clone 1B11-1A8 Analysis of STX1A expression in IMR-32.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-Western-Blot-H00006804-M02-img0010.jpg "Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - STX1A monoclonal antibody (M02), clone 1B11-1A8 Analysis of STX1A expression in IMR-32.")
![Immunocytochemistry/Immunofluorescence: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Analysis of monoclonal antibody to STX1A on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-Immunocytochemistry-Immunofluorescence-H00006804-M02-img0007.jpg)
![Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - STX1A monoclonal antibody (M02), clone 1B11-1A8. Analysis of STX1A expression in PC-12.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-Western-Blot-H00006804-M02-img0009.jpg)
![Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Analysis of STX1A expression in transfected 293T cell line by STX1A monoclonal antibody (M02), clone 1B11-1A8.Lane 1: STX1A transfected lysate(29 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-Western-Blot-H00006804-M02-img0011.jpg)
![ELISA: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Detection limit for recombinant GST tagged STX1A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-1B11-1A8-ELISA-H00006804-M02-img0012.jpg)
![Western Blot: CADPS Antibody [NBP1-77323] - Rat brain tissue lysate with CADPS antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/CADPS-Antibody-Western-Blot-NBP1-77323-img0001.jpg "Western Blot: CADPS Antibody [NBP1-77323] - Rat brain tissue lysate with CADPS antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: CADPS Antibody - BSA Free [NBP1-77323] - Immunofluorescence of CADPS in human brain tissue with CADPS antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77323_rabbit-polyclonal-cadps-antibody-2711202419201575.jpg)
![Immunohistochemistry: CADPS Antibody - BSA Free [NBP1-77323] - Immunohistochemistry of CADPS in human brain with CADPS antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77323_rabbit-polyclonal-cadps-antibody-2711202419541552.jpg)
![Western Blot: SNAP25 Antibody [NB100-1492] - (0.005ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NB100-1492-img0011.jpg "Western Blot: SNAP25 Antibody [NB100-1492] - (0.005ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Western-Blot-NB300-236-img0001.jpg "Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Immunohistochemistry-NB300-236-img0002.jpg)