| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14205 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21809-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54956-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57165-25ul | |||
![Western Blot: PRAS40 Antibody [NBP1-55261] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate](http://images.novusbio.com/fullsize/PRAS40-Antibody-Western-Blot-NBP1-55261-img0004.jpg "Western Blot: PRAS40 Antibody [NBP1-55261] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate")
![Immunohistochemistry: PRAS40 Antibody [NBP1-55261] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic in endothelial cells in blood vessels Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-NBP1-55261-img0003.jpg)
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PRAS40 Antibody (SR1680) [NBP3-21809] - Western blot analysis using NBP3-21809 in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21809_rabbit-pras40-mab-sr1680-57202315234911.jpg "Western Blot: PRAS40 Antibody (SR1680) [NBP3-21809] - Western blot analysis using NBP3-21809 in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PRAS40 Antibody [NBP2-54956] - Staining of human cell line HEK 293 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54956-img0001.jpg "Immunocytochemistry/Immunofluorescence: PRAS40 Antibody [NBP2-54956] - Staining of human cell line HEK 293 shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-57165] - Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-Paraffin-NBP2-57165-img0001.jpg "Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-57165] - Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.")
![Western Blot: PRAS40 Antibody (2Y8Y3) [NBP3-16179] - Western blot analysis of extracts of various cell lines, using AKT1S1/PRAS40 Rabbit mAb (NBP3-16179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PRAS40-Antibody-2Y8Y3-Western-Blot-NBP3-16179-img0001.jpg "Western Blot: PRAS40 Antibody (2Y8Y3) [NBP3-16179] - Western blot analysis of extracts of various cell lines, using AKT1S1/PRAS40 Rabbit mAb (NBP3-16179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")