| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-89182SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-165SS | |||
| Azide and BSA Free | NBP2-80555 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72521 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84791-25ul | |||
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-89182-img0003.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89182-img0002.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of NPC1L1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NPC1L1 antibody generated moderate staining of hepatocyte cytoplasm.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NBP2-89182-img0001.jpg)
![Western Blot: LRH-1/NR5A2 Antibody [NBP2-27204] - Analysis of Lrh-1. HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) RAW lysate probed with 2 ug/ml of Lrh-1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP2-27204-img0003.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP2-27204] - Analysis of Lrh-1. HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) RAW lysate probed with 2 ug/ml of Lrh-1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: LRH-1/NR5A2 Antibody [NBP2-27204] - Simple Western lane view shows a specific band for LRH-1/NR5A2 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Simple-Western-NBP2-27204-img0002.jpg)
![Western Blot: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Western blot analysis of extracts of various cell lines, using SR-B2/LIMPII/SR-B2 Rabbit mAb (NBP3-16779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-7D2P5-Western-Blot-NBP3-16779-img0004.jpg "Western Blot: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Western blot analysis of extracts of various cell lines, using SR-B2/LIMPII/SR-B2 Rabbit mAb (NBP3-16779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded human liver tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-822025208458.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded mouse kidney tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-822025209822.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded Human lung adenocarcinoma tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-8220252010447.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded human thyroid cancer tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-8220252010489.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded mouse spleen tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-822025209857.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded rat kidney tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-822025208418.jpg)
![Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Western-Blot-NBP3-16187-img0005.jpg "Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of HepG2 cells, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Western-Blot-NBP3-16187-img0006.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (4X7X2) [NBP3-16187] - Immunohistochemistry of paraffin-embedded human liver using PON1 Rabbit mAb (NBP3-16187) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Immunohistochemistry-Paraffin-NBP3-16187-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Rat liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-52202511392230.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Mouse liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-5220251255594.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Human liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-5220251242555.jpg)
![Flow Cytometry: ABCA1 Antibody (5A1-1422.11) [NB400-165] - Unpurified [NB400-165] - Analysis of Raw (red) and Raw-cAMP (blue) cells using NB400-165 at a dilution of 1:4000](http://images.novusbio.com/fullsize/ABCA1-Antibody-5A1-1422-11-Flow-Cytometry-NB400-165-img0004.jpg "Flow Cytometry: ABCA1 Antibody (5A1-1422.11) [NB400-165] - Unpurified [NB400-165] - Analysis of Raw (red) and Raw-cAMP (blue) cells using NB400-165 at a dilution of 1:4000")
![Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-29820241420974.jpg "Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunocytochemistry analysis of HeLa cells labeling LIMPII/SR-B2 with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-292024119239.jpg)
![Western Blot: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Western blot analysis of LIMPII/SR-B2 on different lysates with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: SH-SY5Y cell lysate (15 µg/Lane) Lane 4: Neuro-2a cell lysate (15 µg/Lane) Lane 5: MEF cell lysate (15 µg/Lane) Lane 6: PC-12 cell lysate (15 µg/Lane) Lane 7: COS-1 cell lysate (15 µg/Lane) Lane 8: Mouse kidney tissue lysate (30 µg/Lane) Lane 9: Rat kidney tissue lysate (30 µg/Lane) Lane 10: Mouse liver tissue lysate (30 µg/Lane) Lane 11: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 80 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-392024845352.jpg)
![Western Blot: LRH-1/NR5A2 Antibody (3H8) [NBP2-46248] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-3H8-Western-Blot-NBP2-46248-img0002.jpg "Western Blot: LRH-1/NR5A2 Antibody (3H8) [NBP2-46248] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.")
![Immunohistochemistry: LRH-1/NR5A2 Antibody (3H8) [NBP2-46248] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-3H8-Immunohistochemistry-NBP2-46248-img0001.jpg)
![Western Blot: PON1 Antibody (JE30-90) [NBP3-32843] - Western blot analysis of PON1 on different lysates with Rabbit anti-PON1 antibody (NBP3-32843) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32843) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-288202412461639.jpg "Western Blot: PON1 Antibody (JE30-90) [NBP3-32843] - Western blot analysis of PON1 on different lysates with Rabbit anti-PON1 antibody (NBP3-32843) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32843) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PON1 Antibody (JE30-90) [NBP3-32843] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-PON1 antibody (NBP3-32843) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-298202414304136.jpg)
![Flow Cytometry: PON1 Antibody (JE30-90) [NBP3-32843] - Flow cytometric analysis of HepG2 cells labeling PON1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32843, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-29202416453293.jpg)
![Western Blot: PON1 Antibody [NBP2-19893] - . Human tissue extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PON1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PON1-Antibody-Western-Blot-NBP2-19893-img0002.jpg "Western Blot: PON1 Antibody [NBP2-19893] - . Human tissue extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PON1 antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PON1 Antibody [NBP2-19893] - Analysis of mouse liver. PON1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PON1-Antibody-Immunohistochemistry-Paraffin-NBP2-19893-img0001.jpg)
![Western Blot: LRH-1/NR5A2 Antibody [NBP1-32489] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] diluted at 1:500.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP1-32489-img0007.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP1-32489] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: LRH-1/NR5A2 Antibody [NBP1-32489] - Paraformaldehyde-fixed Human ESC, using NR5A2 antibody at 1:400 dilution.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32489-img0004.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody [NBP1-84791] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PON1-Antibody-Immunohistochemistry-Paraffin-NBP1-84791-img0002.jpg "Immunohistochemistry-Paraffin: PON1 Antibody [NBP1-84791] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.")
