| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90816-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49678SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| BSA Free | NBP2-60735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70834 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90815-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43806 | |||
| BSA Free | NBP2-46644-25ul | |||
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***PSample: Paraffin-embedded rat liver.Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16679_rabbit-polyclonal-glutamate-dehydrogenase-antibody-immunohistochemistry-paraffin-261120251981014.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***PSample: Paraffin-embedded rat liver.Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90816 (A) shows similar protein distribution across tissues to independent antibody NBP1-90815 (B).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0016.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90816 (A) shows similar protein distribution across tissues to independent antibody NBP1-90815 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ATPB Antibody [NBP1-90816] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATPB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90816-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody [NBP1-90816] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90816-img0009.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0019.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate immunoreactivity in glomeruli.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0013.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0014.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49678-img0002.jpg "Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Simple Western: PINK1 Antibody [NBP1-49678] - Lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~61kDa. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NBP1-49678-img0006.jpg)
![Immunohistochemistry-Paraffin: PINK1 Antibody [NBP1-49678] - Stain in paraffin embedded mouse brain.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunohistochemistry-Paraffin-NBP1-49678-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested at 1:50 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49678-img0005.jpg)
![Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Western-Blot-NBP2-80831-img0001.jpg "Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80831-img0002.jpg)
![Immunohistochemistry: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80831-img0003.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Simple-Western-NBP2-80831-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Knockout-Validated-NBP2-80831-img0004.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0017.jpg "Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0004.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of human hepatoma. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of mouse skin. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of rat hind brain. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0003.jpg)
![Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Glutamine synthetase antibody [1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [1055] diluted at 1:500. Blue: DAPI](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Frozen-NBP2-43646-img0009.jpg)
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-261120251820022.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-2611202518255016.jpg)
![Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-immunohistochemistry-frozen-2611202518333613.jpg)
![Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-261120251826138.jpg)
![Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:250.Red: GFAP, stained by GFAP antibody diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-immunocytochemistry-immunofluorescence-2611202519365.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] - U87 xenograft, using GSTP1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16756-img0014.jpg)
![GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSTP1 protein stained by GSTP1 antibody (NBP2-16756) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16756_rabbit-polyclonal-glutathione-s-transferase-pi-1-gstp1-antibody-immunocytochemistry-immunofluorescence-2611202518283519.jpg)
![Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Western-Blot-NBP2-02125-img0009.jpg "Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (OTI1F4) [NBP2-02125] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glutamine Synthetase.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-02125-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human Ovary tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0015.jpg)
![Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Jurkat cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Flow-Cytometry-NBP2-02125-img0011.jpg)
![Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutamine Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutamine Synthetase.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Western-Blot-NBP2-02125-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Immunofluorescent staining of HepG2 cells using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-02125-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human breast tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0013.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human endometrium tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0014.jpg)
![Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Hela cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Flow-Cytometry-NBP2-02125-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0008.jpg "Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Indirect staining of rat neuronal/glial cell culture.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0007.jpg)
![Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of rat neuronal/glial cell extract.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunoprecipitation-NB600-1171-img0010.jpg)
![Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta was performed on THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of an ATP Synthase beta monoclonal antibody overnight at 4C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted with 6X Laemmli non-reducing sample buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunoprecipitation-NB600-1171-img0009.jpg)
![Western Blot: PINK1 Antibody [NB600-973] - Lane 1: The primary band with the observed molecular weight of 66 kDa was detected in mouse liver (30ug). Also, a truncated form of the protein at about 33 kDa was detected.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB600-973-img0001.jpg "Western Blot: PINK1 Antibody [NB600-973] - Lane 1: The primary band with the observed molecular weight of 66 kDa was detected in mouse liver (30ug). Also, a truncated form of the protein at about 33 kDa was detected.")
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NB600-973] - LRRK2 accumulates in globules in alphaS tg mice. Double immunofluorescence for alphaS with parkin, PINK1, DJ-1, LRRK2, or negative control (the immunopeptide-preabsorbed anti-LRRK2 antibody) in alphaS tg mice. Scale bar = 5 um for all panels. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-973-img0003.jpg)
![Immunohistochemistry-Paraffin: PINK1 Antibody [NB600-973] - Staining in human liver tissue using a 1:200 dilution.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunohistochemistry-Paraffin-NB600-973-img0002.jpg)
![Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0015.jpg "Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody [NBP1-90815] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90815-img0007.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATPB Antibody [NBP1-90815] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5B antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90815 (A) shows similar protein distribution across tissues to independent antibody NBP1-90816 (B).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0016.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0018.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0019.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0021.jpg)
![Western Blot: Glutamine Synthetase Antibody (CL3004) [NBP2-46644] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GLUL antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-CL3004-Western-Blot-NBP2-46644-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (CL3004) [NBP2-46644] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GLUL antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human kidney shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0006.jpg)
![Western Blot: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Western-Blot-NBP2-46644-img0007.jpg)
![Western Blot: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Western-Blot-NBP2-46644-img0008.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of rat hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of mouse dentate gyrus shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0003.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of rat cerebral cortex shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0004.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human cerebellum shows positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0005.jpg)