| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55753-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13249-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | MAB35262-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55838-25ul | |||
![Western Blot: KIF3A Antibody (1C8K4) [NBP3-16657] - Western blot analysis of extracts of various cell lines, using KIF3A antibody (NBP3-16657) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/KIF3A-Antibody-1C8K4-Western-Blot-NBP3-16657-img0003.jpg "Western Blot: KIF3A Antibody (1C8K4) [NBP3-16657] - Western blot analysis of extracts of various cell lines, using KIF3A antibody (NBP3-16657) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: KIF3A Antibody (1C8K4) [NBP3-16657] - Immunofluorescence analysis of NIH-3T3 cells using KIF3A Rabbit mAb (NBP3-16657) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIF3A-Antibody-1C8K4-Immunocytochemistry-Immunofluorescence-NBP3-16657-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF3A Antibody (1C8K4) [NBP3-16657] - Immunohistochemistry of paraffin-embedded rat testis using KIF3A Rabbit mAb (NBP3-16657) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KIF3A-Antibody-1C8K4-Immunohistochemistry-Paraffin-NBP3-16657-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (1C8K4) [NBP3-16657] - Immunofluorescence analysis of NIH-3T3 cells using KIF3A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16657_rabbit-kif3a-mab-1c8k4-52202511365138.jpg)
![Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-55753] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-55753-img0010.jpg "Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-55753] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-55753] - Staining of human eye retina shows moderate positivity in the photoreceptor layer.](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-55753-img0004.jpg)
![Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-55753] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-55753-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-55753] - Staining of human eye, retina, liver, pancreas and testis using Anti-RPGRIP1 antibody NBP2-55753 (A) shows similar protein distribution across tissues to independent antibody NBP2-13249 (B).](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-55753-img0008.jpg)
![Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-55753] - Staining of human testis shows moderate positivity in late spermatids in seminiferous ducts.](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-55753-img0009.jpg)
![Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-55753] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-55753-img0011.jpg)
![Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-278202415295563.jpg "Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32501) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-298202413585569.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunocytochemistry analysis of NIH/3T3 cells labeling KIF3A with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-29202410334094.jpg)
![Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-13249] - Staining of human eye, retina, liver, pancreas and testis using Anti-RPGRIP1 antibody NBP2-13249 (A) shows similar protein distribution across tissues to independent antibody NBP2-55753 (B).](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-13249-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-13249] - Staining of human eye, retina, liver, pancreas and testis using Anti-RPGRIP1 antibody NBP2-13249 (A) shows similar protein distribution across tissues to independent antibody NBP2-55753 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-13249] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-13249-img0011.jpg)
![Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-13249] - Staining of human eye retina shows strong positivity in the photoreceptor layer.](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-13249-img0009.jpg)
![Immunohistochemistry-Paraffin: RPGRIP1 Antibody [NBP2-13249] - Staining of human testis shows moderate positivity in late spermatids in seminiferous ducts.](http://images.novusbio.com/fullsize/RPGRIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-13249-img0010.jpg)
![Immunohistochemistry-Paraffin: GLI-2 Antibody (524409) [Unconjugated] [MAB35262] - GLI-2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human GLI-2 Monoclonal Antibody (Catalog # MAB35262) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/GLI-2-Antibody-524409-Unconjugated-Immunohistochemistry-Paraffin-MAB35262-img0001.jpg "Immunohistochemistry-Paraffin: GLI-2 Antibody (524409) [Unconjugated] [MAB35262] - GLI-2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human GLI-2 Monoclonal Antibody (Catalog # MAB35262) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.")
![Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB100-1541-img0002.jpg "Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Parvalbumin Antibody [NB100-1541] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Flow-Cytometry-NB100-1541-img0001.jpg)
![Western Blot: Parvalbumin Antibody (C12) [NBP2-79824] - Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Western-Blot-NBP2-79824-img0001.jpg "Western Blot: Parvalbumin Antibody (C12) [NBP2-79824] - Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: Parvalbumin Antibody (C12) [NBP2-79824] - Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Immunohistochemistry-NBP2-79824-img0002.jpg)
![Immunohistochemistry: Parvalbumin Antibody (C12) [NBP2-79824] - Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Immunohistochemistry-NBP2-79824-img0003.jpg)
![Western Blot: Inversin Antibody - BSA Free [NBP3-46466] - MCF7 cells were subjected to SDS PAGE followed by western blot with (Inversin antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-46466_rabbit-inversin-pab-bsa-free-28420251155440.jpg "Western Blot: Inversin Antibody - BSA Free [NBP3-46466] - MCF7 cells were subjected to SDS PAGE followed by western blot with (Inversin antibody) at dilution of 1:100")
![Immunohistochemistry: Inversin Antibody - BSA Free [NBP3-46466] - Immunohistochemistry of paraffin-embedded human breast cancer using (Inversin antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-46466_rabbit-inversin-pab-bsa-free-284202511553134.jpg)
![Immunohistochemistry: RPGR Antibody - BSA Free [NBP3-46691] - Immunohistochemistry of paraffin-embedded human kidney tissue slide using (RPGR Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-46691_rabbit-rpgr-pab-bsa-free-284202512343857.jpg "Immunohistochemistry: RPGR Antibody - BSA Free [NBP3-46691] - Immunohistochemistry of paraffin-embedded human kidney tissue slide using (RPGR Antibody) at dilution of 1:50")
![Western Blot: RPGR Antibody - BSA Free [NBP3-46691] - Mouse eye tissue were subjected to SDS PAGE followed by western blot with (RPGR antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-46691_rabbit-rpgr-pab-bsa-free-284202512343885.jpg)
![Immunohistochemistry-Paraffin: Inversin Antibody [NBP3-41327] - DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10ug/ml NBP3-41327; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41327_rabbit-inversin-pab-612202421243273.jpg "Immunohistochemistry-Paraffin: Inversin Antibody [NBP3-41327] - DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10ug/ml NBP3-41327; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody")
![Western Blot: Inversin Antibody [NBP3-41327] - Sample: Rat Kidney lysate; Primary Ab: 1ug/ml NBP3-41327; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41327_rabbit-inversin-pab-61220242119561.jpg)
![Western Blot: Inversin Antibody [NBP3-41327] - Sample: Recombinant Inversin, Human.](http://images.novusbio.com/fullsize/nbp3-41327_rabbit-inversin-pab-612202421283866.jpg)
![Immunohistochemistry-Paraffin: PACRG Antibody [NBP2-55838] - Staining of human placenta shows low expression as expected.](http://images.novusbio.com/fullsize/PACRG-Antibody-Immunohistochemistry-Paraffin-NBP2-55838-img0003.jpg "Immunohistochemistry-Paraffin: PACRG Antibody [NBP2-55838] - Staining of human placenta shows low expression as expected.")
![Immunohistochemistry-Paraffin: PACRG Antibody [NBP2-55838] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/PACRG-Antibody-Immunohistochemistry-Paraffin-NBP2-55838-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PACRG Antibody [NBP2-55838] - Staining in human fallopian tube and placenta tissues using anti-PACRG antibody. Corresponding PACRG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PACRG-Antibody-Immunohistochemistry-Paraffin-NBP2-55838-img0004.jpg)
![Western Blot: PACRG Antibody [H00135138-D01P] - Analysis of PACRG expression in mouse liver.](http://images.novusbio.com/fullsize/PACRG-Antibody-Western-Blot-H00135138-D01P-img0005.jpg "Western Blot: PACRG Antibody [H00135138-D01P] - Analysis of PACRG expression in mouse liver.")
![Western Blot: PACRG Antibody [H00135138-D01P] - Analysis of PACRG expression in human liver.](http://images.novusbio.com/fullsize/PACRG-Antibody-Western-Blot-H00135138-D01P-img0004.jpg)
![Western Blot: PACRG Antibody [H00135138-D01P] - Analysis of PACRG expression in transfected 293T cell line by PACRG polyclonal antibody.Lane 1: PACRG transfected lysate(29.30 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PACRG-Antibody-Western-Blot-H00135138-D01P-img0006.jpg)