| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27170-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14137-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49408-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50217-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)
![Immunohistochemistry: CYP7B1 Antibody - BSA Free [NBP3-48056] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (CYP7B1 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-48056_rabbit-cyp7b1-pab-bsa-free-284202511501677.jpg "Immunohistochemistry: CYP7B1 Antibody - BSA Free [NBP3-48056] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (CYP7B1 Antibody) at dilution of 1:50")
![Western Blot: CYP7B1 Antibody - BSA Free [NBP3-48056] - A431 cells were subjected to SDS PAGE followed by western blot with (CYP7B1 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-48056_rabbit-cyp7b1-pab-bsa-free-284202511483470.jpg)
![Western Blot: CD1d Antibody (3A6G3) [NBP3-27170] - Analysis using CD1d mAb against HEK293-6e (1) and CD1d (AA: extra 20-301)-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27170_mouse-cd1d-mab-3a6g3-bsa-free-1042024916233.jpg%3Fv=1666705784 "Western Blot: CD1d Antibody (3A6G3) [NBP3-27170] - Analysis using CD1d mAb against HEK293-6e (1) and CD1d (AA: extra 20-301)-hIgGFc transfected HEK293-6e (2) cell lysate.")
![Western Blot: CD1d Antibody (3A6G3) [NBP3-27170] - Analysis using CD1d mAb against human CD1d (AA: extra 20-301) recombinant protein. (Expected MW is 31.9 kDa)](http://images.novusbio.com/fullsize/nbp3-27170_mouse-cd1d-mab-3a6g3-bsa-free-1042024917019.jpg%3Fv=1666705784)
![Flow Cytometry: CD1d Antibody (3A6G3) [NBP3-27170] - Analysis of Jurkat cells using CD1d mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27170_mouse-cd1d-mab-3a6g3-bsa-free-104202491583.jpg%3Fv=1666705784)
![ELISA: CD1d Antibody (3A6G3) [NBP3-27170] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27170_mouse-cd1d-mab-3a6g3-bsa-free-1042024915831.jpg%3Fv=1666705784)
![Western Blot: JAKMIP1 Antibody [NBP2-14137] - Analysis in control (vector only transfected HEK293T lysate) and JAKMIP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/JAKMIP1-Antibody-Western-Blot-NBP2-14137-img0002.jpg "Western Blot: JAKMIP1 Antibody [NBP2-14137] - Analysis in control (vector only transfected HEK293T lysate) and JAKMIP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: JAKMIP1 Antibody [NBP2-14137] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/JAKMIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-14137-img0001.jpg)
![Western Blot: CYP7B1 Antibody (2B11) [H00009420-M06] - Analysis of CYP7B1 expression in transfected 293T cell line by CYP7B1 monoclonal antibody (M06), clone 2B11. Lane 1: CYP7B1 transfected lysatE (58.256 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CYP7B1-Antibody-2B11-Western-Blot-H00009420-M06-img0004.jpg "Western Blot: CYP7B1 Antibody (2B11) [H00009420-M06] - Analysis of CYP7B1 expression in transfected 293T cell line by CYP7B1 monoclonal antibody (M06), clone 2B11. Lane 1: CYP7B1 transfected lysatE (58.256 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CYP7B1 Antibody (2B11) [H00009420-M06] - Analysis of monoclonal antibody to CYP7B1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/CYP7B1-Antibody-2B11-Immunohistochemistry-Paraffin-H00009420-M06-img0003.jpg)
![ELISA: CYP7B1 Antibody (2B11) [H00009420-M06] - Detection limit for recombinant GST tagged CYP7B1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CYP7B1-Antibody-2B11-ELISA-H00009420-M06-img0002.jpg)
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-49408] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-49408-img0003.jpg "Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-49408] - Staining of human testis shows high expression.")
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-49408] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-49408-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-49408] - Staining in human testis and liver tissues using anti-ATG16L1 antibody. Corresponding ATG16L1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-49408-img0004.jpg)
![Western Blot: USP18 Antibody [NB600-893] - Analysis of a band 43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.](http://images.novusbio.com/fullsize/USP18-Antibody-Western-Blot-NB600-893-img0004.jpg "Western Blot: USP18 Antibody [NB600-893] - Analysis of a band 43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.")
![Western Blot:[NBP3-28970]-Detection of human and mouse CD1D by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat and from mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD1D antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-28970_rabbit-cd1d-pab-231202511585525.jpg "Western Blot:[NBP3-28970]-Detection of human and mouse CD1D by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat and from mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD1D antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot:[NBP3-28970]-CD1d Antibody](http://images.novusbio.com/fullsize/nbp3-28970_rabbit-cd1d-pab-31120250364041.jpg)
![Western Blot: CYP7B1 Antibody (M17-P3F2) [NBP2-50217] - Western blot was performed using anti-Cytochrome P450 7B1 [M17-P3F2]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](http://images.novusbio.com/fullsize/CYP7B1-Antibody-M17-P3F2-Western-Blot-NBP2-50217-img0002.jpg "Western Blot: CYP7B1 Antibody (M17-P3F2) [NBP2-50217] - Western blot was performed using anti-Cytochrome P450 7B1 [M17-P3F2]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.")
![Immunohistochemistry-Paraffin: CYP7B1 Antibody (M17-P3F2) [NBP2-50217] - Shows positive cytoplasmic immunostaining in normal liver (A) which is expected as CYP7B1 is involved in bile acid synthesis. Primary colorectal (B) and metastatic colorectal tumors (C) showed stronger staining compared to normal colon mucosa (D).](http://images.novusbio.com/fullsize/CYP7B1-Antibody-M17-P3F2-Immunohistochemistry-Paraffin-NBP2-50217-img0001.jpg)
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP3-14543] - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/ATG16L1 Antibody-Immunohistochemistry-Paraffin-NBP3-14543-img0002.jpg "Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP3-14543] - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Simple Western: ATG16L1 Antibody [NBP3-14543] - ATG16L1 antibody (LS-A11356, 20 mg/mL) yields a specific band on capillary Western analysis (Protein Simple, WES 12-230 kDa separation module) in 10 ng purified recombinant human ATG16L1 protein.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Simple-Western-NBP3-14543-img0001.jpg)