| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47607-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-21805-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB13382-SP | |||
![Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Western-Blot-NBP1-47607-img0006.jpg "Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav beta 2 Monoclonal Antibody, Clone S8B-1 (NBP1-47607). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav beta 2 Monoclonal Antibody (NBP1-47607) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunocytochemistry-Immunofluorescence-NBP1-47607-img0008.jpg)
![Immunohistochemistry: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunohistochemistry-NBP1-47607-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.. Image courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunocytochemistry-Immunofluorescence-NBP1-47607-img0003.jpg)
![ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-32-BSA-Free-ELISA-NBP1-05182-img0001.jpg "ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.")
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunofluorescence-NBP2-21805-img0002.jpg "Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Immunohistochemistry: N-Cadherin Antibody (GC-4) [NBP2-21805] - Frozen acetone fixed chicken cardiac sections stained with Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunohistochemistry-NBP2-21805-img0001.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)
![Western Blot: Syndecan-4 Antibody [NBP2-24630] - Analysis of Syndecan 4 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) Raw cell lysate using NBP2-24630 at 1.0 ug/ml.](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-Western-Blot-NBP2-24630-img0003.jpg "Western Blot: Syndecan-4 Antibody [NBP2-24630] - Analysis of Syndecan 4 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) Raw cell lysate using NBP2-24630 at 1.0 ug/ml.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-970106-Immunocytochemistry-Immunofluorescence-MAB13382-img0001.jpg "Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.")
![Western Blot: DOCK7 Antibody [NBP1-78748] - A549 human alveolar adenocarcinoma cell line. Total A549 cell extract diluted to 1/4 in Laemmli buffer. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DOCK7-Antibody-Western-Blot-NBP1-78748-img0003.jpg "Western Blot: DOCK7 Antibody [NBP1-78748] - A549 human alveolar adenocarcinoma cell line. Total A549 cell extract diluted to 1/4 in Laemmli buffer. Western blot image submitted by a verified customer review.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DOCK7 Antibody [NBP1-78748] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-DOCK7 antibody used for WB at 0.1 mcg/mL (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. DOCK7 was also immunoprecipitated by rabbit anti-DOCK7 antibody (NBP1-78749) which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/DOCK7-Antibody-Western-Blot-NBP1-78748-img0002.jpg)
![Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody [NBP2-20315] - Human gastric cancer, using SERPINE2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SERPINE2-Antibody-Immunohistochemistry-Paraffin-NBP2-20315-img0003.jpg "Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody [NBP2-20315] - Human gastric cancer, using SERPINE2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Serpin E2/PN1 Antibody [NBP2-20315] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-Western-Blot-NBP2-20315-img0010.jpg)
![Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27201-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27197-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: SCN7A Antibody [NB100-81029] - Staining of sodium channel protein type 7 subunit alpha in human sperm cells.](http://images.novusbio.com/fullsize/SCN7A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81029-img0002.jpg "Immunocytochemistry/Immunofluorescence: SCN7A Antibody [NB100-81029] - Staining of sodium channel protein type 7 subunit alpha in human sperm cells.")
![Immunohistochemistry-Paraffin: SCN7A Antibody [NB100-81029] - Staining of normal human heart tissue formalin fixed and paraffin embedded.](http://images.novusbio.com/fullsize/SCN7A-Antibody-Immunohistochemistry-Paraffin-NB100-81029-img0001.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0009.jpg "Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of monoclonal antibody to SNAP23 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunohistochemistry-Paraffin-H00008773-M01-img0006.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - SNAP23 expression in Raw 264.7.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0001.jpg)
![Immunoprecipitation: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 transfected lysate using anti-SNAP23 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SNAP23 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunoprecipitation-H00008773-M01-img0007.jpg)
![Sandwich ELISA: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Detection limit for recombinant GST tagged SNAP23 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Sandwich-ELISA-H00008773-M01-img0008.jpg)