| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB72272-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21849-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08510 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03603-20ul | |||
![Immunocytochemistry/Immunofluorescence: eNOS Antibody [NBP3-23210] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23210_rabbit-enos-pab-1992023141014.jpg "Immunocytochemistry/Immunofluorescence: eNOS Antibody [NBP3-23210] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.")
![Western Blot: eNOS Antibody [NBP3-23210] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23210_rabbit-enos-pab-19920231856933.jpg)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-61220242126297.jpg "Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: TDO2 Antibody (C2) [NBP3-41854] - Sample: Lane1: Porcine Liver lysate; Lane2: Rat Liver lysate Primary Ab: 2ug/ml NBP3-41854; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-612202421243225.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Skeletal muscle Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-612202421121318.jpg)
![Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] - P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-91020242011451.jpg "Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] - P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:500.Blue: Hoechst 33342 staining.")
![Western Blot: P2Y2 Antibody [NBP2-15060] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-91020242001015.jpg)
![Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-western-blot-261120251827013.jpg)
![Western Blot: PAK1 Antibody (SR2113) [NBP3-21849] - Western blot analysis using NBP3-21849 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21849_rabbit-pak1-mab-sr2113-57202316582145.jpg "Western Blot: PAK1 Antibody (SR2113) [NBP3-21849] - Western blot analysis using NBP3-21849 in A431 cell lysate.")
![Western Blot: CREB3 Antibody [H00010488-D01P] - Analysis of CREB3 recombinant protein by CREB3 purified antibody.](http://images.novusbio.com/fullsize/CREB3-Antibody-Western-Blot-H00010488-D01P-img0003.jpg "Western Blot: CREB3 Antibody [H00010488-D01P] - Analysis of CREB3 recombinant protein by CREB3 purified antibody.")
![Western Blot: CREB3 Antibody [H00010488-D01P] - Analysis of CREB3 expression in transfected 293T cell line by CREB3 polyclonal antibody.Lane 1: CREB3 transfected lysate(41.40 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CREB3-Antibody-Western-Blot-H00010488-D01P-img0004.jpg)
![Proximity Ligation Assay: CREB3 Antibody [H00010488-D01P] - Analysis of protein-protein interactions between CREB3 and CREB3L4. HeLa cells were stained with anti-CREB3 rabbit purified polyclonal 1:1200 and anti-CREB3L4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CREB3-Antibody-Proximity-Ligation-Assay-H00010488-D01P-img0002.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Tonsil stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0005.jpg "Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Tonsil stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).")
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0001.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0002.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0003.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0004.jpg)
![Western Blot: SELENBP1 Antibody [NBP3-03603] - Analysis of extracts of various cell lines, using SELENBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP3-03603-img0004.jpg "Western Blot: SELENBP1 Antibody [NBP3-03603] - Analysis of extracts of various cell lines, using SELENBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: SELENBP1 Antibody [NBP3-03603] - Analysis of HeLa cells using SELENBP1 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03603-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SELENBP1 Antibody [NBP3-03603] - Analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using SELENBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. BBlocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Knockout-Validated-NBP3-03603-img0003.jpg)