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![Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Western-Blot-NBP2-37288-img0003.jpg "Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Flow cytometric analysis of Hela cells using PPP1R1B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Flow-Cytometry-NBP2-37288-img0004.jpg)
![Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against human PPP1R1B (AA: 95-204) recombinant protein. (Expected MW is 38.3 kDa)](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-Western-Blot-NBP2-37288-img0002.jpg)
![ELISA: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/DARPP-32-Antibody-2G6D9-ELISA-NBP2-37288-img0001.jpg)
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-61595] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Monomer-Type-1-Protein-In-vitro-assay-NBP2-61595-img0004.jpg "In vitro assay: Recombinant Mouse alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-61595] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.")
![Western Blot: Ubiquitin B Antibody (3C12) [NBP2-37521] - Western blot analysis using UBB mouse mAb against NIH/3T3 (1) and Hela (2) cell lysate.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-3C12-Western-Blot-NBP2-37521-img0003.jpg "Western Blot: Ubiquitin B Antibody (3C12) [NBP2-37521] - Western blot analysis using UBB mouse mAb against NIH/3T3 (1) and Hela (2) cell lysate.")
![Flow Cytometry: Ubiquitin B Antibody (3C12) [NBP2-37521] - Flow cytometric analysis of Hela cells using UBB mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-3C12-Flow-Cytometry-NBP2-37521-img0004.jpg)
![Western Blot: Ubiquitin B Antibody (3C12) [NBP2-37521] - Western blot analysis using UBB mAb against human UBB (AA: 1-299) recombinant protein. (Expected MW is 26 kDa)](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-3C12-Western-Blot-NBP2-37521-img0002.jpg)
![ELISA: Ubiquitin B Antibody (3C12) [NBP2-37521] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-3C12-ELISA-NBP2-37521-img0001.jpg)
![Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-288202412461614.jpg "Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-298202414304124.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32847, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-392024884664.jpg)
![Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-288202412461627.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (NBP3-32811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32811, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241492636.jpg)
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241420935.jpg)
![Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-278202415242658.jpg "Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: RCAN1 Antibody (JE62-71) [NBP3-32100] - Immunocytochemistry analysis of Jurkat cells labeling RCAN1 with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202410234244.jpg)
![Flow Cytometry: RCAN1 Antibody (JE62-71) [NBP3-32100] - Flow cytometric analysis of Jurkat cells labeling RCAN1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32100, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202416372121.jpg)
![Western Blot: CRMP4 Antibody [NBP2-87213] - WB Suggested Anti-DPYSL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T](http://images.novusbio.com/fullsize/CRMP4-Antibody-Western-Blot-NBP2-87213-img0003.jpg "Western Blot: CRMP4 Antibody [NBP2-87213] - WB Suggested Anti-DPYSL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T")
![Immunohistochemistry: CRMP4 Antibody [NBP2-87213] - Immunohistochemistry with Small intestine, submucosal plexus tissue. Antibody concentration: 10ug/ml](http://images.novusbio.com/fullsize/CRMP4-Antibody-Immunohistochemistry-NBP2-87213-img0001.jpg)
![Immunoprecipitation: CRMP4 Antibody [NBP2-87213] - DRYSL3 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with NBP2-87213 with 1:200 dilution. Western blot was performed using NBP2-87213 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: DRYSL3 IP with NBP2-87213](http://images.novusbio.com/fullsize/CRMP4-Antibody-Immunoprecipitation-NBP2-87213-img0004.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![In vitro assay: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril (NBP3-14774) is the majority of the seed.](http://images.novusbio.com/fullsize/Recombinant-Rat-alpha-Synuclein-Active--Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14774-img0001.jpg "In vitro assay: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril (NBP3-14774) is the majority of the seed.")
![Electron Microscopy: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - TEM of Rat alpha-Synuclein Pre-formed Fibrils (PFFS) (NBP3-14774). 500nm](http://images.novusbio.com/fullsize/Recombinant Rat alpha-Synuclein Active- Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14774-img0003.jpg)
![Electron Microscopy: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - TEM of Rat alpha-Synuclein Pre-formed Fibrils (PFFS) (NBP3-14774). 200nm](http://images.novusbio.com/fullsize/Recombinant-Rat-alpha-Synuclein-Active--Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14774-img0002.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers . A much greater increase in fluorescence is seen when 100 uM monomer is combined with 10 uM of fibrils (NBP3-14767) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-In-vitro-assay-NBP3-14767-img0001.jpg "In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers . A much greater increase in fluorescence is seen when 100 uM monomer is combined with 10 uM of fibrils (NBP3-14767) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.")
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - TEM of A53T alpha synuclein Pre-formed Fibrils (NBP3-14767)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-Electron-Microscopy-NBP3-14767-img0003.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - TEM of A53T alpha synuclein Pre-formed Fibrils (NBP3-14767)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-Electron-Microscopy-NBP3-14767-img0002.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Acetyl (N-Term) Protein [NBP3-14769] - Thioflavin T assay for N-terminal acetylated alpha synuclein monomers and pre-formed fibrils (PFFs) (NBP3-14769). Increased fluorescence, indicative of beta-sheet formation, was seen when 50 ug N-acetylated monomer and 5 ug N-acetylated fibril were combined, compared to monomer or fibril alone.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Acetyl-N-Term-Protein-In-vitro-assay-NBP3-14769-img0001.jpg "In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Acetyl (N-Term) Protein [NBP3-14769] - Thioflavin T assay for N-terminal acetylated alpha synuclein monomers and pre-formed fibrils (PFFs) (NBP3-14769). Increased fluorescence, indicative of beta-sheet formation, was seen when 50 ug N-acetylated monomer and 5 ug N-acetylated fibril were combined, compared to monomer or fibril alone.")
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Acetyl (N-Term) Protein [NBP3-14769] - TEM of N-terminal acetylated alpha synuclein pre-formed fibirls (PFFs) (NBP3-14769)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Acetyl-N-Term-Protein-Electron-Microscopy-NBP3-14769-img0003.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Acetyl (N-Term) Protein [NBP3-14769] - TEM of N-terminal acetylated alpha synuclein pre-formed fibirls (PFFs) (NBP3-14769)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Acetyl-N-Term-Protein-Electron-Microscopy-NBP3-14769-img0002.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-61220242126297.jpg "Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: TDO2 Antibody (C2) [NBP3-41854] - Sample: Lane1: Porcine Liver lysate; Lane2: Rat Liver lysate Primary Ab: 2ug/ml NBP3-41854; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-612202421243225.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Skeletal muscle Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-612202421121318.jpg)