| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61775-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-44375-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37281-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47664-0.1mg | |||
| IHC-Prediluted | NBP2-48253 | |||
![Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Sample Type: Human Fetal Brain Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Western-Blot-NBP1-80061-img0007.jpg "Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Sample Type: Human Fetal Brain Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry: GABA-A R rho 2 Antibody [NBP1-80061] - Formalin Fixed Paraffin Embedded Tissue: Human Adult heart Observed Staining: Membrane(tight junctions - intercalated disks) Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec Protocol located in Reviews and Data.](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Immunohistochemistry-NBP1-80061-img0004.jpg)
![Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Hela cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Western-Blot-NBP1-80061-img0002.jpg)
![Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Sample Type: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Western-Blot-NBP1-80061-img0005.jpg)
![Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Sample Type: 293T Antibody Dilution: 1.0 ug/ml GABRR2 is supported by BioGPS gene expression data to be expressed in HEK293T](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Western-Blot-NBP1-80061-img0006.jpg)
![Immunohistochemistry: GABA-A R rho 2 Antibody [NBP1-80061] - Human Adult heart Observed Staining: Membrane (tight junctions - intercalated disks) Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 s](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Immunohistochemistry-NBP1-80061-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-62000-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).")
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human prostate. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-62000-img0002.jpg)
![Flow Cytometry: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TRP1-Antibody-Flow-Cytometry-NBP2-62000-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-62000-img0004.jpg)
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human cortex. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-62000-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of SH-SY5Y cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411531046.jpg "Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of SH-SY5Y cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PAC1R Antibody [NBP3-38030] - Western blot analysis of lysates from Mouse heart , using PAC1R Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-10122024120621.jpg)
![Western Blot: PAC1R Antibody [NBP3-38030] - Western blot analysis of lysates from U-87MG cells, using PAC1R Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411535820.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of NIH/3T3 cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-10122024120660.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of C6 cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411535868.jpg)
![Western Blot: CEND1 Antibody (1D3Z1) [NBP3-16614] - Western blot analysis of extracts of various cell lines, using CEND1 Rabbit mAb (NBP3-16614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Western-Blot-NBP3-16614-img0003.jpg "Western Blot: CEND1 Antibody (1D3Z1) [NBP3-16614] - Western blot analysis of extracts of various cell lines, using CEND1 Rabbit mAb (NBP3-16614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Immunofluorescence analysis of mouse brain cells using CEND1 Rabbit mAb (NBP3-16614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Immunocytochemistry-Immunofluorescence-NBP3-16614-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Immunofluorescence analysis of rat brain cells using CEND1 Rabbit mAb (NBP3-16614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Immunocytochemistry-Immunofluorescence-NBP3-16614-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Confocal imaging of paraffin-embedded Rat brain tissue using CEND1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16614_rabbit-cend1-mab-1d3z1-5220251242528.jpg)
![Immunocytochemistry/ Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Confocal imaging of paraffin-embedded Mouse brain tissue using CEND1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16614_rabbit-cend1-mab-1d3z1-52202511383719.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0004.jpg "Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunohistochemistry-Paraffin: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of rectum cancer tissues using GRIA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Immunohistochemistry-NBP2-61775-img0003.jpg)
![Flow Cytometry: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Flow-Cytometry-NBP2-61775-img0002.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 35-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0005.jpg)
![ELISA: GluR2 Antibody (7A7A3) [NBP2-61775] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-ELISA-NBP2-61775-img0001.jpg)
![Western Blot: UT2/SLC14A2 Antibody [NBP1-44375] - Western blot analysis of Rat Inner medulla showing detection of UT2/SLC14A2 protein using Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375). Primary Antibody: Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375) at 1:1000.](http://images.novusbio.com/fullsize/UT2-SLC14A2-Antibody-Western-Blot-NBP1-44375-img0005.jpg "Western Blot: UT2/SLC14A2 Antibody [NBP1-44375] - Western blot analysis of Rat Inner medulla showing detection of UT2/SLC14A2 protein using Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375). Primary Antibody: Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375) at 1:1000.")
![Immunohistochemistry: UT2/SLC14A2 Antibody [NBP1-44375] - Immunohistochemistry analysis using Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375). Tissue: kidney tissue. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C)UT2/SLC14A2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/UT2-SLC14A2 Antibody-Immunohistochemistry-NBP1-44375-img0006.jpg)
![Immunohistochemistry-Paraffin: UT2/SLC14A2 Antibody [NBP1-44375] - kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody (NBP1-44375) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C)UT-A1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/UT2-SLC14A2-Antibody-Immunohistochemistry-Paraffin-NBP1-44375-img0004.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0003.jpg "Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunofluorescence analysis of Hela cells using GABBR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunocytochemistry-Immunofluorescence-NBP2-37281-img0004.jpg)
![Immunohistochemistry: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using GABBR2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunohistochemistry-NBP2-37281-img0005.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against human GABBR2 (AA: 319-483) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0002.jpg)
![ELISA: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-ELISA-NBP2-37281-img0001.jpg)
![Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-278202415212420.jpg "Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-29820241420994.jpg)
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32650, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-298202414262675.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PS02-10) [NBP3-32650] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-2920241192341.jpg)
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47970-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - HEK293 cells using Neurofilament Monoclonal Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47970-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (RT-97 + NR-4) [NBP2-45280] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (RT-97 + NR-4).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT-97-+-NR-4-Immunohistochemistry-Paraffin-NBP2-45280-img0002.jpg "Immunohistochemistry-Paraffin: NF-H Antibody (RT-97 + NR-4) [NBP2-45280] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (RT-97 + NR-4).")
![Flow Cytometry: NF-H Antibody (RT-97 + NR-4) [NBP2-45280] - Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (RT-97 + NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT-97-+-NR-4-Flow-Cytometry-NBP2-45280-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (RT-97 + NR-4) [NBP2-45280] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (RT-97 + NR-4).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT-97-+-NR-4-Immunohistochemistry-Paraffin-NBP2-45280-img0001.jpg)