| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92915-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61775-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42202-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59341-25ug | |||
![Western Blot: ALDH1A2 Antibody [NBP2-92915] - Analysis of extracts of various cell lines, using ALDH1A2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ALDH1A2-Antibody-Western-Blot-NBP2-92915-img0004.jpg "Western Blot: ALDH1A2 Antibody [NBP2-92915] - Analysis of extracts of various cell lines, using ALDH1A2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: ALDH1A2 Antibody [NBP2-92915] - Analysis of NIH-3T3 cells using ALDH1A2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ALDH1A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92915-img0002.jpg)
![Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP2-92915] - Rat kidney using ALDH1A2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/ALDH1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-92915-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ALDH1A2 Antibody [NBP2-92915] - Analysis of C6 cells using ALDH1A2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ALDH1A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92915-img0001.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0004.jpg "Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunohistochemistry-Paraffin: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of rectum cancer tissues using GRIA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Immunohistochemistry-NBP2-61775-img0003.jpg)
![Flow Cytometry: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Flow-Cytometry-NBP2-61775-img0002.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 35-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0005.jpg)
![ELISA: GluR2 Antibody (7A7A3) [NBP2-61775] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-ELISA-NBP2-61775-img0001.jpg)
![Western Blot: KCNK3 Antibody (S374-48) [NBP2-42202] - Western Blot analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~50 kDa.](http://images.novusbio.com/fullsize/KCNK3-Antibody-S374-48-Western-Blot-NBP2-42202-img0004.jpg "Western Blot: KCNK3 Antibody (S374-48) [NBP2-42202] - Western Blot analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~50 kDa.")
![Immunocytochemistry/Immunofluorescence: KCNK3 Antibody (S374-48) [NBP2-42202] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TASK1 Potassium Channel Antibody (D) Composite.](http://images.novusbio.com/fullsize/KCNK3-Antibody-S374-48-Immunocytochemistry-Immunofluorescence-NBP2-42202-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK3 Antibody (S374-48) [NBP2-42202] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/KCNK3-Antibody-S374-48-Immunocytochemistry-Immunofluorescence-NBP2-42202-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK3 Antibody (S374-48) [NBP2-42202] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TASK1 Potassium Channel Antibody. (D) Composite.](http://images.novusbio.com/fullsize/KCNK3-Antibody-S374-48-Immunocytochemistry-Immunofluorescence-NBP2-42202-img0006.jpg)
![Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Western-Blot-NBP2-59341-img0009.jpg "Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000.")
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0012.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0010.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0011.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0013.jpg)
![Western Blot: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2B.](http://images.novusbio.com/fullsize/ITM2B-Antibody-OTI1C11-Azide-and-BSA-Free-Western-Blot-NBP2-71460-img0001.jpg "Western Blot: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2B.")
![Immunohistochemistry: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - Staining of paraffin-embedded Human Kidney tissue using anti-ITM2B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ITM2B-Antibody-OTI1C11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71460-img0004.jpg)
![Flow Cytometry: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - Analysis of Jurkat cells, using anti-ITM2B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ITM2B-Antibody-OTI1C11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71460-img0003.jpg)
![Flow Cytometry: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - Analysis of Hela cells, using anti-ITM2B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ITM2B-Antibody-OTI1C11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71460-img0002.jpg)
![Western Blot: IP3R1 Antibody [NBP3-09066] - Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Western-Blot-NBP3-09066-img0001.jpg "Western Blot: IP3R1 Antibody [NBP3-09066] - Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP3-09066] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP3-09066-img0003.jpg)
![Flow Cytometry: IP3R1 Antibody [NBP3-09066] - Flow cytometric analysis of SH-SY-5Y cells with IP3 Receptor antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Flow-Cytometry-NBP3-09066-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP3-09066] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP3-09066-img0002.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-278202415212487.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-2982024149266.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-292024119233.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32389, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-29202416372194.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-27820241631677.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32388) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-298202414161846.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-29202410234266.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32388, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-2920241645329.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29820241420955.jpg "Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-298202413585572.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunocytochemistry analysis of HeLa cells labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 Alpha 1 antibody (NBP3-33143) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29202410234287.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Western blot analysis of Sodium Potassium ATPase Alpha 1 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/100,000 dilution. Lane 1: HeLa cell lysate (no heat) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane) Lane 5: L-929 cell lysate (no heat) (20 µg/Lane) Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-392024848504.jpg)
![Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Analysis of PSMA1 expression in HeLa.](http://images.novusbio.com/fullsize/PSMA1-Antibody-1D9-1C7-Western-Blot-H00005682-M01-img0008.jpg "Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Analysis of PSMA1 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of monoclonal antibody to PSMA1 on HeLa cell. Antibody concentration 60 ug/ml.](http://images.novusbio.com/fullsize/PSMA1-Antibody-1D9-1C7-Immunocytochemistry-Immunofluorescence-H00005682-M01-img0006.jpg)
![Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of PSMA1 over-expressed 293 cell line, cotransfected with PSMA1 Validated Chimera RNAi ( Cat # H00005682-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMA1 monoclonal antibody (M01), clone 1D9-1C7 (Cat # H00005682-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PSMA1-Antibody-1D9-1C7-Western-Blot-H00005682-M01-img0007.jpg)
![Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody (M01), clone 1D9-1C7.Lane 1: PSMA1 transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PSMA1-Antibody-1D9-1C7-Western-Blot-H00005682-M01-img0009.jpg)
![Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of TR alpha/NR1A1/Thyroid Hormone Receptor alpha protein using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Load: 10 ug. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band(s): Higher molceular weight bands could be due to PTMs.](http://images.novusbio.com/fullsize/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-H43-Western-Blot-NBP2-79822-img0002.jpg "Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of TR alpha/NR1A1/Thyroid Hormone Receptor alpha protein using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Load: 10 ug. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band(s): Higher molceular weight bands could be due to PTMs.")
![Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-H43-Immunocytochemistry-Immunofluorescence-NBP2-79822-img0005.jpg)
![Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunohistochemistry analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 4X.](http://images.novusbio.com/fullsize/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-H43-Immunohistochemistry-NBP2-79822-img0004.jpg)
![Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunohistochemistry analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 7.5X.](http://images.novusbio.com/fullsize/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-H43-Immunohistochemistry-NBP2-79822-img0003.jpg)