| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38021-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33785-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88933-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58941-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39086-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86491-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94663-0.02ml | |||
![Western Blot: NDUFA7 Antibody [NBP2-57928] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/NDUFA7-Antibody-Western-Blot-NBP2-57928-img0002.jpg "Western Blot: NDUFA7 Antibody [NBP2-57928] - Western blot analysis in human cell line RT-4.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-57928] - Staining in human heart muscle and pancreas tissues using anti-NDUFA7 antibody. Corresponding NDUFA7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDUFA7-Antibody-Immunohistochemistry-Paraffin-NBP2-57928-img0005.jpg)
![Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-57928] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/NDUFA7-Antibody-Immunohistochemistry-Paraffin-NBP2-57928-img0003.jpg)
![Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-57928] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/NDUFA7-Antibody-Immunohistochemistry-Paraffin-NBP2-57928-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MT-ND3 Antibody [NBP2-38021] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38021-img0003.jpg "Immunocytochemistry/Immunofluorescence: MT-ND3 Antibody [NBP2-38021] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-38021] - Staining in human heart muscle and lymph node tissues using anti-MT-ND3 antibody. Corresponding MT-ND3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-38021-img0006.jpg)
![Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-38021] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-38021-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-38021] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-38021-img0005.jpg)
![Immunohistochemistry-Paraffin: Ryanodine Receptor 1 Antibody [NBP2-33785] - Staining in human skeletal muscle and colon tissues using anti-RYR1 antibody. Corresponding RYR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ryanodine-Receptor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-33785-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ryanodine Receptor 1 Antibody [NBP2-33785] - Staining in human skeletal muscle and colon tissues using anti-RYR1 antibody. Corresponding RYR1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Ryanodine Receptor 1 Antibody [NBP2-33785] - Staining of human cell line SiHa shows localization to cytosol & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ryanodine-Receptor-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33785-img0002.jpg)
![Immunohistochemistry-Paraffin: Ryanodine Receptor 1 Antibody [NBP2-33785] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/Ryanodine-Receptor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-33785-img0005.jpg)
![Immunohistochemistry-Paraffin: Ryanodine Receptor 1 Antibody [NBP2-33785] - Staining of human colon shows low expression as expected.](http://images.novusbio.com/fullsize/Ryanodine-Receptor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-33785-img0004.jpg)
![Western Blot: NDUFA5 Antibody [NBP1-88933] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/NDUFA5-Antibody-Western-Blot-NBP1-88933-img0005.jpg "Western Blot: NDUFA5 Antibody [NBP1-88933] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: NDUFA5 Antibody [NBP1-88933] - Staining of human colon shows strong cytoplasmic positivity(granular pattern) in glandular cells.](http://images.novusbio.com/fullsize/NDUFA5-Antibody-Immunohistochemistry-Paraffin-NBP1-88933-img0002.jpg)
![Western Blot: NDUFA5 Antibody [NBP1-88933] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/NDUFA5-Antibody-Western-Blot-NBP1-88933-img0004.jpg)
![Western Blot: FTO Antibody [NBP2-58941] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.](http://images.novusbio.com/fullsize/FTO-Antibody-Western-Blot-NBP2-58941-img0002.jpg "Western Blot: FTO Antibody [NBP2-58941] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FTO Antibody [NBP2-58941] - Staining in human parathyroid gland and pancreas tissues using anti-FTO antibody. Corresponding FTO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-58941-img0006.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody [NBP2-58941] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-58941-img0004.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody [NBP2-58941] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-58941-img0005.jpg)
![Western Blot: MLC1 Antibody [NBP2-39086] - Analysis in control (vector only transfected HEK293T lysate) and MLC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MLC1-Antibody-Western-Blot-NBP2-39086-img0006.jpg "Western Blot: MLC1 Antibody [NBP2-39086] - Analysis in control (vector only transfected HEK293T lysate) and MLC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MLC1 Antibody [NBP2-39086] - Staining in human cerebral cortex and pancreas tissues using anti-MLC1 antibody. Corresponding MLC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP2-39086-img0005.jpg)
![Immunohistochemistry-Paraffin: MLC1 Antibody [NBP2-39086] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP2-39086-img0003.jpg)
![Immunohistochemistry-Paraffin: MLC1 Antibody [NBP2-39086] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP2-39086-img0004.jpg)
![Immunohistochemistry-Paraffin: NAPG Antibody [NBP1-86491] - Staining in human cerebral cortex and skeletal muscle tissues using anti-NAPG antibody. Corresponding NAPG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NAPG-Antibody-Immunohistochemistry-Paraffin-NBP1-86491-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NAPG Antibody [NBP1-86491] - Staining in human cerebral cortex and skeletal muscle tissues using anti-NAPG antibody. Corresponding NAPG RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NAPG Antibody [NBP1-86491] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/NAPG-Antibody-Immunohistochemistry-Paraffin-NBP1-86491-img0006.jpg)
![Immunohistochemistry-Paraffin: NAPG Antibody [NBP1-86491] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NAPG-Antibody-Immunohistochemistry-Paraffin-NBP1-86491-img0007.jpg)
![Western Blot: UCP3 Antibody [NBP2-94663] - Analysis of extracts of various cell lines, using UCP3 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/UCP3-Antibody-Western-Blot-NBP2-94663-img0001.jpg "Western Blot: UCP3 Antibody [NBP2-94663] - Analysis of extracts of various cell lines, using UCP3 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: UCP3 Antibody - Azide and BSA Free [UCP3] - Immunofluorescence analysis of C6 cells using UCP3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94663_rabbit-polyclonal-ucp3-antibody-52202516334124.jpg)
![Immunocytochemistry/ Immunofluorescence: UCP3 Antibody - Azide and BSA Free [UCP3] - Immunofluorescence analysis of HeLa cells using UCP3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94663_rabbit-polyclonal-ucp3-antibody-52202516345267.jpg)
![Western Blot: UCP3 Antibody - Azide and BSA Free [UCP3] - Western blot analysis of various lysates using UCP3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-94663_rabbit-polyclonal-ucp3-antibody-5220251640257.jpg)
![Immunocytochemistry/ Immunofluorescence: UCP3 Antibody - Azide and BSA Free [UCP3] - Immunofluorescence analysis of NIH/3T3 cells using UCP3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94663_rabbit-polyclonal-ucp3-antibody-5220251639694.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Western blot analysis of Dihydrolipoamide Dehydrogenase/DLD on different lysates with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: SK-Br-3 cell lysate (20 ug/Lane) Lane 7: U-937 cell lysate (20 ug/Lane) Lane 8: HepG2 cell lysate (20 ug/Lane) Lane 9: NIH/3T3 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32539) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32539_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-psh0-83-27820241521245.jpg "Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Western blot analysis of Dihydrolipoamide Dehydrogenase/DLD on different lysates with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: SK-Br-3 cell lysate (20 ug/Lane) Lane 7: U-937 cell lysate (20 ug/Lane) Lane 8: HepG2 cell lysate (20 ug/Lane) Lane 9: NIH/3T3 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32539) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32539) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32539_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-psh0-83-29820241492614.jpg)
![Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunocytochemistry analysis of HeLa cells labeling Dihydrolipoamide Dehydrogenase/DLD with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32539_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-psh0-83-29202410234295.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].")
![<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract | D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0001.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0002.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0003.jpg)
![Immunohistochemistry;Dihydrolipoamide Dehydrogenase/DLD Antibody[NBP3-30341]Detection of human DLD by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DLD used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30391_rabbit-dihydrolipoamide-dehydrogenase-dld-pab-78202410153550.jpg "Immunohistochemistry;Dihydrolipoamide Dehydrogenase/DLD Antibody[NBP3-30341]Detection of human DLD by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DLD used at a dilution of 1:1,000 . Detection: DAB")
![Immunohistochemistry;Dihydrolipoamide Dehydrogenase/DLD Antibody[NBP3-30391]Detection of human DLD by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DLD used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30391_rabbit-dihydrolipoamide-dehydrogenase-dld-pab-78202410372614.jpg)
![Immunoprecipitation:Dihydrolipoamide Dehydrogenase/DLD AntibodyNBP3-30391] -Detection of human DLD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DLD antibody NBP3-30391 (lot NBP3-30391-1) used for IP at 6 µg per reaction. DLD was also immunoprecipitated by rabbit anti-DLD antibody. For blotting immunoprecipitated DLD, NBP3-30391 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-30391_rabbit-dihydrolipoamide-dehydrogenase-dld-pab-3012202418203110.jpg)
![Immunohistochemistry;ATP5A Antibody[NBP3-29711]Detection of mouse ATP5A1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29712_rabbit-atp5a-pab-78202410153527.jpg "Immunohistochemistry;ATP5A Antibody[NBP3-29711]Detection of mouse ATP5A1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB")
![Immunohistochemistry;ATP5A Antibody[NBP3-29712]Detection of human ATP5A1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29712_rabbit-atp5a-pab-782024936118.jpg)
![Immunoprecipitation:ATP5A AntibodyNBP3-29712] -Detection of human ATP5A1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP5A1 antibody NBP3-29712 (lot NBP3-29712-1) used for IP at 6 µg per reaction. ATP5A1 was also immunoprecipitated by rabbit anti-ATP5A1.. For blotting immunoprecipitated ATP5A1, NBP3-29712 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29712_rabbit-atp5a-pab-301220241633611.jpg)