| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57015-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14227 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84437-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64867-0.025mg | |||
![Immunohistochemistry-Paraffin: CD28 Antibody [NBP2-57015] - Analysis in human lymph node and liver tissues. Corresponding CD28 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD28-Antibody-Immunohistochemistry-Paraffin-NBP2-57015-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD28 Antibody [NBP2-57015] - Analysis in human lymph node and liver tissues. Corresponding CD28 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD28 Antibody [NBP2-57015] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/CD28-Antibody-Immunohistochemistry-Paraffin-NBP2-57015-img0010.jpg)
![Immunohistochemistry-Paraffin: CD28 Antibody [NBP2-57015] - Staining of human liver shows no positivity in hepatocytes.](http://images.novusbio.com/fullsize/CD28-Antibody-Immunohistochemistry-Paraffin-NBP2-57015-img0006.jpg)
![Immunohistochemistry-Paraffin: CD28 Antibody [NBP2-57015] - Staining of human tonsil shows moderate membranous positivity in lymphocytes.](http://images.novusbio.com/fullsize/CD28-Antibody-Immunohistochemistry-Paraffin-NBP2-57015-img0007.jpg)
![Immunohistochemistry-Paraffin: CD28 Antibody [NBP2-57015] - Staining of human lymph node shows moderate membranous positivity in lymphocytes.](http://images.novusbio.com/fullsize/CD28-Antibody-Immunohistochemistry-Paraffin-NBP2-57015-img0009.jpg)
![Western Blot: Ki67/MKI67 Antibody [NBP3-05538] - Western blot analysis of equal amounts of cell lysates probed with Ki67/MKI67 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67/MKI67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67/MKI67 is a rather unstable snd sjhort half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Western-Blot-NBP3-05538-img0002.jpg "Western Blot: Ki67/MKI67 Antibody [NBP3-05538] - Western blot analysis of equal amounts of cell lysates probed with Ki67/MKI67 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67/MKI67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67/MKI67 is a rather unstable snd sjhort half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.")
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NBP3-05538] - Immunofluorescent analysis of HeLa cells stained with Ki67/MKI67 Antibody at a dilution of 1:2,000 in red, and mouse mAb to beta-tubulin at a dilution of 1:5,000 in green. The blue is DAPI staining of nuclear DNA. The Ki67/MKI67 antibody detects Ki67/MKI67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67/MKI67 forms a coat around condensed chromosomes. Ki67/MKI67 is not detected in cells in the quiescent G0 state. The beta-tubulin antibody produces strong staining of cytoplasmic microtubules.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05538-img0001.jpg)
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/7081R) [NBP3-13834] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with p16INK4a/CDKN2A antibody (CDKN2A/7081R).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-7081R-Immunohistochemistry-Paraffin-NBP3-13834-img0002.jpg "Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/7081R) [NBP3-13834] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with p16INK4a/CDKN2A antibody (CDKN2A/7081R).")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/7081R) [NBP3-13834] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with p16INK4a/CDKN2A antibody (CDKN2A/7081R).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-7081R-Immunohistochemistry-Paraffin-NBP3-13834-img0001.jpg)
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP1-84437] - Staining in human skin and skeletal muscle tissues using anti-CALML5 antibody. Corresponding CALML5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP1-84437-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CALML5 Antibody [NBP1-84437] - Staining in human skin and skeletal muscle tissues using anti-CALML5 antibody. Corresponding CALML5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CALML5 Antibody [NBP1-84437] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/CALML5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84437-img0006.jpg)
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP1-84437] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP1-84437-img0007.jpg)
![Western Blot: Proteasome 20S C2 Antibody [NB300-624] - Analysis of CHO cell extract.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Western-Blot-NB300-624-img0003.jpg "Western Blot: Proteasome 20S C2 Antibody [NB300-624] - Analysis of CHO cell extract.")
![Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-27820241631618.jpg "Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32584) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-298202414304162.jpg)
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunocytochemistry analysis of U-2 OS cells treated with or without 10μM NUTLIN 3A for 24 hours labeling MDM2/HDM2 with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-29202410484835.jpg)
![Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-NB100-77315-img0006.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.")
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].")
![<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract | D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0001.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0002.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0003.jpg)
![Western Blot: STRN4 Antibody [NBP2-36537] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Striatin-4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/STRN4-Antibody-Western-Blot-NBP2-36537-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: STRN4 Antibody [NBP2-36537] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Striatin-4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: STRN4 Antibody [NBP2-36537] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated Striatin-4, A304-573A was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/STRN4-Antibody-Immunoprecipitation-NBP2-36537-img0002.jpg)
![Western Blot: STRN4 Antibody [NBP3-13187] - Various tissue extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STRN4 antibody (NBP3-13187) diluted at 1:2000.](http://images.novusbio.com/fullsize/STRN4-Antibody-Western-Blot-NBP3-13187-img0002.jpg "Western Blot: STRN4 Antibody [NBP3-13187] - Various tissue extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STRN4 antibody (NBP3-13187) diluted at 1:2000.")
![Western Blot: STRN4 Antibody [NBP3-13187] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STRN4 antibody (NBP3-13187) diluted at 1:1000.](http://images.novusbio.com/fullsize/STRN4-Antibody-Western-Blot-NBP3-13187-img0001.jpg)
![Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Analysis using the PE conjugate of NB100-64867. Staining of human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD28-Antibody-YTH913-12-Flow-Cytometry-NB100-64867-img0007.jpg "Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Analysis using the PE conjugate of NB100-64867. Staining of human peripheral blood lymphocytes.")
![Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Staining of human peripheral blood lymphocytes with Rat anti Human.](http://images.novusbio.com/fullsize/CD28-Antibody-YTH913-12-Flow-Cytometry-NB100-64867-img0003.jpg)
![Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Staining of human peripheral blood lymphocytes with Rat anti Human.](http://images.novusbio.com/fullsize/CD28-Antibody-YTH913-12-Flow-Cytometry-NB100-64867-img0004.jpg)
![Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Analysis using the Biotin conjugate of NB100-64867. Staining of human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD28-Antibody-YTH913-12-Flow-Cytometry-NB100-64867-img0006.jpg)
![Western Blot: CPEB3 Antibody [NBP1-56919] - Analysis of 721_B whole cell Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml lysate Quantity: 25ug/ Lane Gel Concentration: 0.12% .](http://images.novusbio.com/fullsize/CPEB3-Antibody-Western-Blot-NBP1-56919-img0004.jpg "Western Blot: CPEB3 Antibody [NBP1-56919] - Analysis of 721_B whole cell Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml lysate Quantity: 25ug/ Lane Gel Concentration: 0.12% .")
![Western Blot: CPEB3 Antibody [NBP1-56919] - 721_B cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPEB3-Antibody-Western-Blot-NBP1-56919-img0002.jpg)