| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05194-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77326-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-206SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto594 | NBP3-14765-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38391-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38390-25ul | |||
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-15365-img0027.jpg "<b>Independent Antibodies Validation. </b>Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Rat brain. alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Mouse hippocampus. alpha Synuclein stained by alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0033.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Human colon carcinoma, using alpha Synuclein antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0006.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha-Synuclein antibody (NBP2-15365) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242024611.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - alpha-Synuclein antibody detects alpha-Synuclein protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.alpha-Synuclein stained by alpha-Synuclein antibody (NBP2-15365) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242021977.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - alpha-Synuclein antibody detects alpha-Synuclein protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.alpha-Synuclein stained by alpha-Synuclein antibody (NBP2-15365) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-91020242091454.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunohistochemistry-frozen-261120251834269.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunocytochemistry-immunofluorescence-2611202518351723.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15365_rabbit-polyclonal-alpha-synuclein-antibody-immunohistochemistry-frozen-261120251831548.jpg)
![Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0004.jpg "Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.")
![Simple Western: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Synuclein-alpha Antibody (2A7) [NBP1-05194] - Simple Western lane view shows a specific band for alpha-Synuclein in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Simple-Western-NBP1-05194-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Western blot analysis of tissue lysates using mAb to alpha-synuclein NBP1-05194. dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. Strong band at about 15kDa corresponds to alpha-synuclein protein.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Western-Blot-NBP1-05194-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Mixed neuron-glial cultures stained with NBP1-05194, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal antibody to MAP2 NB300-213 (green). The alpha-synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0002.jpg)
![Flow Cytometry: alpha-Synuclein Antibody (2A7) [NBP1-05194] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NBP1-05194AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Flow-Cytometry-NBP1-05194-img0006.jpg)
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen sectioned adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Frozen-NBP2-20857-img0009.jpg "Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen sectioned adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - CL1-5 xenograft, using VGAT antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-20857-img0003.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - VIAAT/SLC32A1/VGAT antibody [N1N2], N-term detects VIAAT/SLC32A1/VGAT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: VIAAT/SLC32A1/VGAT stained by VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-910202420134040.jpg)
![Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-western-blot-261120251825265.jpg)
![Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-western-blot-261120251826364.jpg)
![VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: VGAT protein stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-immunohistochemistry-frozen-261120251825312.jpg)
![VGAT antibody [N1N2], N-term detects VGAT protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-immunocytochemistry-immunofluorescence-26112025196525.jpg)
![VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VGAT protein stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-immunocytochemistry-immunofluorescence-2611202518491317.jpg)
![Western Blot: LIMPII/SR-B2 Antibody [NBP1-77326] - Analysis in mouse liver tissue lysate with antibody at 1 ug/mL in (A) the absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Western-Blot-NBP1-77326-img0005.jpg "Western Blot: LIMPII/SR-B2 Antibody [NBP1-77326] - Analysis in mouse liver tissue lysate with antibody at 1 ug/mL in (A) the absence and (B) presence of blocking peptide.")
![Simple Western: LIMPII/SR-B2 Antibody [NBP1-77326] - Whole cell lysates from the indicated cell lines were probed, at the indicated concentrations, with a 1:25 dilution of antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Simple-Western-NBP1-77326-img0007.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody - BSA Free [NBP1-77326] - Immunohistochemistry of LIMPII/SR-B2 in human skeletal muscle tissue with LIMPII/SR-B2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77326_rabbit-polyclonal-limpii-sr-b2-antibody-2711202419451212.jpg)
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody - BSA Free [NBP1-77326] - Immunofluorescence of LIMPII/SR-B2 in Human Skeletal Muscle tissue with LIMPII/SR-B2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77326_rabbit-polyclonal-limpii-sr-b2-antibody-2711202419333447.jpg)
![Western Blot: HIP1 Related Antibody (1E1) [NB300-206] - Analysis of HIP1 Related on HeLa whole cell extract.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-1E1-Western-Blot-NB300-206-img0001.jpg "Western Blot: HIP1 Related Antibody (1E1) [NB300-206] - Analysis of HIP1 Related on HeLa whole cell extract.")
![Immunohistochemistry-Paraffin: HIP1 Related Antibody (1E1) [NB300-206] - Analysis of a FFPE tissue section of human kidney using 1:200 concentration of HIP1 Related antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This HIP1 related antibody generated strong staining of the cytoplasm and membrane.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-1E1-Immunohistochemistry-Paraffin-NB300-206-img0002.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-212024938316.jpg "Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Red: NF-H, a Cytoplasm marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249425448.jpg)
![Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249362775.jpg)
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249415958.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - Non-transfected (-) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (NBP3-25743) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249415994.jpg)
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249422680.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249362722.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (NBP3-25743) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249403895.jpg)
![Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VIAAT/SLC32A1/VGAT antibody [HL1615] (NBP3-25743) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-western-blot-141120251832021.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Western-Blot-NBP2-54787-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54787-img0006.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of active alpha Synuclein monomer (NBP2-54788) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787), or 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-In-vitro-assay-NBP2-54787-img0007.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) (Catalog No. NBP2-54787) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Product-Image-NBP2-54787-img0009.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - TEM of Type 2 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54787)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Electron-Microscopy-NBP2-54787-img0008.jpg)
![Western Blot: HIP1 Related Antibody [NBP2-38391] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Western-Blot-NBP2-38391-img0003.jpg "Western Blot: HIP1 Related Antibody [NBP2-38391] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: HIP1 Related Antibody [NBP2-38391] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38391-img0004.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human kidney.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0008.jpg)
![Immunohistochemistry: HIP1 Related Antibody [NBP2-38391] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-NBP2-38391-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIP1R antibody NBP2-38391 (A) shows similar protein distribution across tissues to independent antibody NBP2-38390 (B).](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0005.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human testis.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0006.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human colon.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0007.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38391] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38391-img0009.jpg)
![Western Blot: HIP1 Related Antibody [NBP2-38390] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Western-Blot-NBP2-38390-img0003.jpg "Western Blot: HIP1 Related Antibody [NBP2-38390] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: HIP1 Related Antibody [NBP2-38390] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38390-img0004.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human testis.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0008.jpg)
![Immunohistochemistry: HIP1 Related Antibody [NBP2-38390] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-NBP2-38390-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIP1R antibody NBP2-38390 (A) shows similar protein distribution across tissues to independent antibody NBP2-38391 (B).](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0005.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human colon.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0006.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0007.jpg)
![Immunohistochemistry-Paraffin: HIP1 Related Antibody [NBP2-38390] - Staining of human kidney.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Immunohistochemistry-Paraffin-NBP2-38390-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0005.jpg "Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] -Analysis of HeLa cells labeling Phospho-alpha Synuclein (S129) with Rabbit anti-Phospho-alpha Synuclein (S129) antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein (S129) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-immunocytochemistry-immunofluorescence-1820247526..jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] -Analysis of Neuro-2a cells labeling Phospho-alpha Synuclein with Rabbit anti-Phospho-alpha Synuclein antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-immunocytochemistry-immunofluorescence-18202475444..jpg)
![Flow Cytometry:alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of HeLa cells labeling Phospho-alpha Synuclein (S129).Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-flow-cytometry-18202475655..jpg)
![Western Blot: pan-Synuclein Antibody [NBP3-13218] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with pan-Synuclein antibody (NBP3-13218) diluted at 1:1000.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Western-Blot-NBP3-13218-img0001.jpg "Western Blot: pan-Synuclein Antibody [NBP3-13218] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with pan-Synuclein antibody (NBP3-13218) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: pan-Synuclein protein stained by pan-Synuclein antibody (NBP3-13218) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13218-img0005.jpg)
![Immunohistochemistry-Frozen: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: pan-Synuclein protein stained by pan-Synuclein antibody (NBP3-13218) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Frozen-NBP3-13218-img0006.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. pan-Synuclein antibody (NBP3-13218) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP3-13218-img0002.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. pan-Synuclein antibody (NBP3-13218) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP3-13218-img0003.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP3-13218) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13218_rabbit-pan-synuclein-pab-immunohistochemistry-frozen-26112025183681.jpg)