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![Western Blot: Orexin Receptor 1/2 Antibody [NB100-93465] - Staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Orexin-Receptor-1-2-Antibody-Western-Blot-NB100-93465-img0003.jpg "Western Blot: Orexin Receptor 1/2 Antibody [NB100-93465] - Staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Connexin 43/GJA1 Antibody [NBP1-51938] - NBP1-51938 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Connexin-43-GJA1-Antibody-Western-Blot-NBP1-51938-img0001.jpg "Western Blot: Connexin 43/GJA1 Antibody [NBP1-51938] - NBP1-51938 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NB100-40807-img0001.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Western Blot: GRID1 Antibody (1A9) [H00002894-M01] - GRID1 monoclonal antibody (M01), clone 1A9 Analysis of GRID1 expression in HeLa.](http://images.novusbio.com/fullsize/GRID1-Antibody-1A9-Western-Blot-H00002894-M01-img0004.jpg "Western Blot: GRID1 Antibody (1A9) [H00002894-M01] - GRID1 monoclonal antibody (M01), clone 1A9 Analysis of GRID1 expression in HeLa.")
![Sandwich ELISA: GRID1 Antibody (1A9) [H00002894-M01] - Detection limit for recombinant GST tagged GRID1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GRID1-Antibody-1A9-Sandwich-ELISA-H00002894-M01-img0003.jpg)
![Immunohistochemistry: Synaptoporin Antibody - BSA Free [NBP3-45770] - Immunohistochemistry of paraffin-embedded human gliomas tissue slide using (Synaptoporin Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-45770_rabbit-synaptoporin-pab-bsa-free-284202512565616.jpg "Immunohistochemistry: Synaptoporin Antibody - BSA Free [NBP3-45770] - Immunohistochemistry of paraffin-embedded human gliomas tissue slide using (Synaptoporin Antibody) at dilution of 1:50")
![Western Blot: Synaptoporin Antibody - BSA Free [NBP3-45770] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (Synaptoporin antibody) at dilution of 1:800](http://images.novusbio.com/fullsize/nbp3-45770_rabbit-synaptoporin-pab-bsa-free-28420251372028.jpg)
![Western Blot: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SHG-44 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32359_rabbit-gaba-b-r1-mab-je60-09-278202415212436.jpg "Western Blot: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SHG-44 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32359_rabbit-gaba-b-r1-mab-je60-09-298202414333648.jpg)
![Western Blot: Cofilin Antibody (PSH0-34) [NBP3-32205] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32205) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF-7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (30 ug/Lane) Lane 10: VERO cell lysate (30 ug/Lane) Lane 11: NIH/3T3 cell lysate (22 ug/Lane) Lane 12: PC-12 cell lysate (19 ug/Lane) Lane 13: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 3 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32205) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32205_rabbit-cofilin-mab-psh0-34-278202415295596.jpg "Western Blot: Cofilin Antibody (PSH0-34) [NBP3-32205] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32205) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF-7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (30 ug/Lane) Lane 10: VERO cell lysate (30 ug/Lane) Lane 11: NIH/3T3 cell lysate (22 ug/Lane) Lane 12: PC-12 cell lysate (19 ug/Lane) Lane 13: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 3 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32205) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Cofilin Antibody (PSH0-34) [NBP3-32205] - Flow cytometric analysis of Hela cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32205, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32205_rabbit-cofilin-mab-psh0-34-29202416453265.jpg)
![Western Blot: Cofilin Antibody (PSH0-38) [NBP3-32206] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32206) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (30 ug/Lane) Lane 10: VERO cell lysate (30 ug/Lane) Lane 11: NIH/3T3 cell lysate (22 ug/Lane) Lane 12: PC-12 cell lysate (30 ug/Lane) Lane 13: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32206) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32206_rabbit-cofilin-mab-psh0-38-27820241631698.jpg "Western Blot: Cofilin Antibody (PSH0-38) [NBP3-32206] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32206) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (30 ug/Lane) Lane 10: VERO cell lysate (30 ug/Lane) Lane 11: NIH/3T3 cell lysate (22 ug/Lane) Lane 12: PC-12 cell lysate (30 ug/Lane) Lane 13: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32206) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Cofilin Antibody (PSH0-38) [NBP3-32206] - Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32206, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32206_rabbit-cofilin-mab-psh0-38-392024884638.jpg)
![Western Blot: HAP40 Antibody [NBP3-42324] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-42324_rabbit-hap40-pab-612202421395392.jpg "Western Blot: HAP40 Antibody [NBP3-42324] - Sample: Recombinant protein.")
![Western Blot: HAP40 Antibody [NBP3-42324] - Sample: Mouse Cerebrum lysate; Primary Ab: 5ug/ml NBP3-42324; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42324_rabbit-hap40-pab-612202421175916.jpg)
![Immunohistochemistry: MOK Antibody - BSA Free [NBP3-47469] - Immunohistochemistry of paraffin-embedded rat heart using (MOK Antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-47469_rabbit-mok-pab-bsa-free-28420251363717.jpg "Immunohistochemistry: MOK Antibody - BSA Free [NBP3-47469] - Immunohistochemistry of paraffin-embedded rat heart using (MOK Antibody) at dilution of 1:100")
![Western Blot: MOK Antibody - BSA Free [NBP3-47469] - HeLa cells were subjected to SDS PAGE followed by western blot with (MOK antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47469_rabbit-mok-pab-bsa-free-28420251304137.jpg)
![ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP3-25953] - A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25953_rabbit-igg2b-mab-rm108-azide-and-bsa-free-22620242311048.jpg "ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP3-25953] - A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![Western Blot: IgG2b Antibody (RM108) - Azide and BSA Free [NBP3-25953] - Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using Clone RM108. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced g2b form (~50 kDa).](http://images.novusbio.com/fullsize/nbp3-25953_rabbit-igg2b-mab-rm108-azide-and-bsa-free-24620241332462.jpg)
![ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP3-25953] - ELISA of mouse immunoglobulins shows RM108 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25953_rabbit-igg2b-mab-rm108-azide-and-bsa-free-2462024132625.jpg)