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![Immunoprecipitation:UQCRQ AntibodyNBP3-30631] -Detection of human UQCRQ by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UQCRQ antibody (NBP3-30631 lot 1) used for IP at 2 µg per reaction. UQCRQ was also immunoprecipitated by rabbit anti-UQCRQ.. For blotting immunoprecipitated UQCRQ, NBP3-30631 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30631_rabbit-uqcrq-pab-3012202412392147.jpg "Immunoprecipitation:UQCRQ AntibodyNBP3-30631] -Detection of human UQCRQ by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UQCRQ antibody (NBP3-30631 lot 1) used for IP at 2 µg per reaction. UQCRQ was also immunoprecipitated by rabbit anti-UQCRQ.. For blotting immunoprecipitated UQCRQ, NBP3-30631 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-western-blot-2942025165210..jpg "Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Rabbit Tau [p Thr217] mAb (HL1276) [NBP3-45156] - Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (p Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (p Thr217) antibody [HL1276] (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-elisa-2942025164816..jpg)
![Immunoprecipitation:UQCRC2 AntibodyNBP3-29635] -Detection of human UQCRC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UQCRC2 antibody NBP3-29635 (lot NBP3-29635-1) used for IP at 6 µg per reaction. UQCRC2 was also immunoprecipitated by rabbit anti-UQCRC2 antibody. For blotting immunoprecipitated UQCRC2, NBP3-29635 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29635_rabbit-uqcrc2-pab-301220241828460.jpg "Immunoprecipitation:UQCRC2 AntibodyNBP3-29635] -Detection of human UQCRC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UQCRC2 antibody NBP3-29635 (lot NBP3-29635-1) used for IP at 6 µg per reaction. UQCRC2 was also immunoprecipitated by rabbit anti-UQCRC2 antibody. For blotting immunoprecipitated UQCRC2, NBP3-29635 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot:[NBP3-29969]-Detection of human and mouse ATP5C1 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATP5C1 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29969_rabbit-atpg-pab-231202511585532.jpg "Western Blot:[NBP3-29969]-Detection of human and mouse ATP5C1 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATP5C1 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:HIP1 Related AntibodyNBP3-30685] -Detection of human HIP1R/HIP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HIP1R/HIP12 antibody NBP3-30685 (lot NBP3-30685-1) used for IP at 6 µg per reaction. HIP1R/HIP12 was also immunoprecipitated by rabbit anti-HIP1R/HIP12.. For blotting immunoprecipitated HIP1R/HIP12, NBP3-30685 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30685_rabbit-hip1-related-pab-301220241332429.jpg "Immunoprecipitation:HIP1 Related AntibodyNBP3-30685] -Detection of human HIP1R/HIP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HIP1R/HIP12 antibody NBP3-30685 (lot NBP3-30685-1) used for IP at 6 µg per reaction. HIP1R/HIP12 was also immunoprecipitated by rabbit anti-HIP1R/HIP12.. For blotting immunoprecipitated HIP1R/HIP12, NBP3-30685 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot:[NBP3-29664]-Detection of human and mouse TMED10 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMED10 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29664_rabbit-tmp21-p23-pab-2312025121173.jpg "Western Blot:[NBP3-29664]-Detection of human and mouse TMED10 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMED10 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot:[NBP3-29665]-Detection of human and mouse TMED10 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMED10 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29665_rabbit-tmp21-p23-pab-231202512101642.jpg "Western Blot:[NBP3-29665]-Detection of human and mouse TMED10 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMED10 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Wild-type (WT) and HTT knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody [HL2483] (NBP3-48688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48688_rabbit-huntingtin-mab-hl2483-western-blot-141120251840523.jpg "<b>Genetic Strategies Validation. </b>Wild-type (WT) and HTT knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody [HL2483] (NBP3-48688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody [HL2483] (NBP3-48688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48688_rabbit-huntingtin-mab-hl2483-western-blot-1411202518365910.jpg)
![Immunoprecipitation:NDUFA5 AntibodyNBP3-29884] -Detection of human NDUFA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFA5 antibody NBP3-29884 (lot NBP3-29884-1) used for IP at 6 µg per reaction. NDUFA5 was also immunoprecipitated by rabbit anti-NDUFA5 antibody. For blotting immunoprecipitated NDUFA5, NBP3-29884 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp3-29884_rabbit-ndufa5-pab-3012202415311911.jpg "Immunoprecipitation:NDUFA5 AntibodyNBP3-29884] -Detection of human NDUFA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFA5 antibody NBP3-29884 (lot NBP3-29884-1) used for IP at 6 µg per reaction. NDUFA5 was also immunoprecipitated by rabbit anti-NDUFA5 antibody. For blotting immunoprecipitated NDUFA5, NBP3-29884 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Western Blot: Nogo Receptor/NgR Antibody [NB100-1536] - (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nogo-Receptor-NgR-Antibody-Western-Blot-NB100-1536-img0002.jpg "Western Blot: Nogo Receptor/NgR Antibody [NB100-1536] - (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.")