| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44858 | |||
| Azide and BSA Free | NBP2-34430-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54664-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48450 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65362-0.025mg | |||
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Western Blot: CD45 Antibody (SPM569 + SPM570) [NBP2-34383] - Western Blot analysis of human spleen tissue lysates using CD45 antibody (SPM569 + SPM570) [NBP2-34383]. Observed molecular weight ~150 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-SPM569-+-SPM570-Western-Blot-NBP2-34383-img0004.jpg "Western Blot: CD45 Antibody (SPM569 + SPM570) [NBP2-34383] - Western Blot analysis of human spleen tissue lysates using CD45 antibody (SPM569 + SPM570) [NBP2-34383]. Observed molecular weight ~150 kDa.")
![Immunohistochemistry-Paraffin: CD45 Antibody (SPM569 + SPM570) [NBP2-34383] - Formalin-fixed, paraffin-embedded human Lymphoma stained with CD45 antibody (SPM569 + SPM570) [NBP2-34383].](http://images.novusbio.com/fullsize/CD45-Antibody-SPM569-+-SPM570-Immunohistochemistry-Paraffin-NBP2-34383-img0003.jpg)
![Western Blot: MTH1 Antibody [NBP2-54664] - Analysis in control (vector only transfected HEK293T lysate) and NUDT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MTH1-Antibody-Western-Blot-NBP2-54664-img0005.jpg "Western Blot: MTH1 Antibody [NBP2-54664] - Analysis in control (vector only transfected HEK293T lysate) and NUDT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NBP2-54664] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/MTH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54664-img0004.jpg)
![Immunohistochemistry-Paraffin: MTH1 Antibody [NBP2-54664] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MTH1-Antibody-Immunohistochemistry-Paraffin-NBP2-54664-img0001.jpg)
![Western Blot: MTH1 Antibody [NBP2-54664] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/MTH1-Antibody-Western-Blot-NBP2-54664-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody [NBP1-88103] - Staining in human tonsil and pancreas tissues using CD45 antibody [NBP1-88103]. Corresponding CD45 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-Paraffin-NBP1-88103-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD45 Antibody [NBP1-88103] - Staining in human tonsil and pancreas tissues using CD45 antibody [NBP1-88103]. Corresponding CD45 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD45 Antibody [NBP1-88103] - Staining of human pancreas with CD45 antibody [NBP1-88103] shows low expression as expected.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-Paraffin-NBP1-88103-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody [NBP1-88103] - Staining of human tonsil with CD45 antibody [NBP1-88103] shows high expression.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-Paraffin-NBP1-88103-img0006.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - p53R2 antibody [HL1400] detects p53R2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. p53R2 stained by p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25588_rabbit-p53r2-mab-hl1400-azide-and-bsa-free-2120249393598.jpg "Immunohistochemistry-Paraffin: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - p53R2 antibody [HL1400] detects p53R2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. p53R2 stained by p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25588_rabbit-p53r2-mab-hl1400-azide-and-bsa-free-2120249393576.jpg)
![Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25588_rabbit-p53r2-mab-hl1400-azide-and-bsa-free-212024939353.jpg)
![Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25588_rabbit-p53r2-mab-hl1400-azide-and-bsa-free-2120249432893.jpg)
![Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-EM-05-Flow-Cell-Surface-NBP1-44763-img0002.jpg "Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.")
![Western Blot: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/1131).](http://images.novusbio.com/fullsize/CD45RA-Antibody-PTPRC-1131-Azide-and-BSA-Free-Western-Blot-NBP2-54336-img0002.jpg "Western Blot: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/1131).")
![Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336] - Formalin-fixed paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (PTPRC/1131).](http://images.novusbio.com/fullsize/CD45RA-Antibody-PTPRC-1131-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54336-img0001.jpg)
![Immunohistochemistry: MTH1 Antibody (PSH02-40) [NBP3-32612] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (NBP3-32612) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32612) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32612_rabbit-mth1-mab-psh02-40-298202414333693.jpg "Immunohistochemistry: MTH1 Antibody (PSH02-40) [NBP3-32612] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (NBP3-32612) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32612) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: MTH1 Antibody (PSH02-40) [NBP3-32612] - Flow cytometric analysis of Jurkat cells labeling MTH1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32612,1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32612_rabbit-mth1-mab-psh02-40-392024884620.jpg)
![Western Blot: MTH1 Antibody (PSH02-40) [NBP3-32612] - Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (NBP3-32612) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: K-562 cell lysate Lane 6: A549 cell lysate Lane 7: MCF7 cell lysate Lane 8: SW480 cell lysate Lane 9: THP-1 cell lysate Lane 10: SK-OV-3 cell lysate Lane 11: U-2 OS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32612_rabbit-mth1-mab-psh02-40-392024848508.jpg)
![Western Blot: ATM Antibody (2C1-RB) [NBP3-13655] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-RB-Western-Blot-NBP3-13655-img0002.jpg "Western Blot: ATM Antibody (2C1-RB) [NBP3-13655] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ATM Antibody (2C1-RB) [NBP3-13655] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-RB-Western-Blot-NBP3-13655-img0001.jpg)
![Immunohistochemistry-Paraffin: ATM Antibody (2C1-RB) [NBP3-13655] - ATM antibody [2C1-RB] detects ATM protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.ATM stained by ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13655_rabbit-atm-mab-2c1-rb-91020242063372.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATM Antibody (2C1-RB) [NBP3-13655] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13655_rabbit-atm-mab-2c1-rb-1610202419405781.jpg)
![Western Blot: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Western-Blot-NBP3-13705-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Immunocytochemistry-Immunofluorescence-NBP3-13705-img0003.jpg)
![Immunohistochemistry-Paraffin: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Immunohistochemistry-Paraffin-NBP3-13705-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0005.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].")
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the palatine tonsil using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0003.jpg)