| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83032-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48494-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34711-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35106-20ul | |||
![Immunocytochemistry/Immunofluorescence: KNTC1 Antibody [NBP1-83032] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KNTC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83032-img0006.jpg "Immunocytochemistry/Immunofluorescence: KNTC1 Antibody [NBP1-83032] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032] - Staining of human tonsil shows weak cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/KNTC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83032-img0010.jpg)
![Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.](http://images.novusbio.com/fullsize/KNTC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83032-img0005.jpg)
![Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/KNTC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83032-img0007.jpg)
![Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/KNTC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83032-img0008.jpg)
![Immunohistochemistry-Paraffin: KNTC1 Antibody [NBP1-83032] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/KNTC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83032-img0009.jpg)
![Western Blot: Survivin Antibody [NBP2-48494] - Western blot using [NBP2-48494]. Analysis in control (vector only transfected HEK293T lysate) and BIRC5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells). Note: predicted molecular weight of survivin is 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NBP2-48494-img0008.jpg "Western Blot: Survivin Antibody [NBP2-48494] - Western blot using [NBP2-48494]. Analysis in control (vector only transfected HEK293T lysate) and BIRC5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells). Note: predicted molecular weight of survivin is 16 kDa.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Immunohistochemical analysis in formalin-fixed paraffin-embedded human testis and skeletal muscle tissues using Survivin Antibody [NBP2-48494]. Corresponding BIRC5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0007.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Staining of formalin-fixed paraffin-embedded human skeletal muscle using Survivin Antibody [NBP2-48494] shows no nuclear positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0003.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Staining of formalin-fixed paraffin-embedded human skin using Survivin Antibody [NBP2-48494] shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0004.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Staining of formalin-fixed paraffin-embedded human placenta using Survivin Antibody [NBP2-48494] shows moderate to strong nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0005.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Staining of formalin-fixed paraffin-embedded human testis using Survivin Antibody [NBP2-48494] shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0006.jpg)
![Western Blot: PCM1 Antibody [NB100-58828] - Detection of mouse PCM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCM1-Antibody-Western-Blot-NB100-58828-img0004.jpg "Western Blot: PCM1 Antibody [NB100-58828] - Detection of mouse PCM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PCM1 Antibody [NB100-58828] - Detection of Human and Mouse PCM1 on HeLa whole cell lysate using NB100-58828. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58829.](http://images.novusbio.com/fullsize/PCM1-Antibody-Western-Blot-NB100-58828-img0002.jpg)
![Western Blot: PCM1 Antibody [NB100-58828] - Detection of human PCM1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/PCM1-Antibody-Western-Blot-NB100-58828-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCM1 Antibody [NB100-58828] - Detection of human PCM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCM1 antibody NB100-58828 (lot NB100-58828-2) used for IP at 6 ug per reaction. PCM1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-58828-1) and rabbit anti-PCM1 antibody NB100-58829. For blotting immunoprecipitated PCM1, NB100-58828 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PCM1-Antibody-NB100-58828-img0005.jpg)
![Western Blot: H2AX Antibody [NBP2-27427] - Analysis of MOLT4 lysate in the 1) absence, 2) presence of immunizing peptide using Histone H2A.x antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/H2AX-Antibody-Western-Blot-NBP2-27427-img0003.jpg "Western Blot: H2AX Antibody [NBP2-27427] - Analysis of MOLT4 lysate in the 1) absence, 2) presence of immunizing peptide using Histone H2A.x antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: H2AX Antibody [NBP2-27427] - Formalin-fixed, paraffin-embedded human spleen stained with Histone H2A.x antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunohistochemistry-Paraffin-NBP2-27427-img0004.jpg)
![Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Western-Blot-NBP2-34331-img0003.jpg "Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Analysis using the Azide and BSA Free version of NBP2-34331. Staining of human Tonsil stained with Cdk1 MAb (POH-1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Immunohistochemistry-Paraffin-NBP2-34331-img0001.jpg)
![Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Western-Blot-NBL1-13748-img0003.jpg "Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.")
![Western Blot: Nucleophosmin Overexpression Lysate (Adult Normal) [NBL1-13748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleophosmin.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13748-img0002.jpg)
![Western Blot: Kif4A Antibody [NBP3-35106] - Western blot analysis of various lysates using Kif4A Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241252857.jpg "Western Blot: Kif4A Antibody [NBP3-35106] - Western blot analysis of various lysates using Kif4A Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Human stomach using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-10122024120688.jpg)
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241252836.jpg)
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-10122024120624.jpg)
![Immunocytochemistry/ Immunofluorescence: Kif4A Antibody [NBP3-35106] - Immunofluorescence analysis of HeLa cells using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241157269.jpg)
![Immunohistochemistry: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32669) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-298202414161882.jpg "Immunohistochemistry: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32669) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunocytochemistry analysis of LOVO cells labeling NPM1 with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-2920241114844.jpg)
![Flow Cytometry: NPM1 Antibody (PD00-91) [NBP3-32669] - Flow cytometric analysis of Daudi cells labeling NPM1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32669, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-2920241637216.jpg)
![Western Blot: NUP98 Antibody [NB100-93325] - Detection of Human NUP96 on HeLa whole cell lysate using NB100-93325. NUP96 was also immunoprecipitated by rabbit anti-NUP96 antibody NB100-93326.](http://images.novusbio.com/fullsize/NUP98-Antibody-Western-Blot-NB100-93325-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NUP98 Antibody [NB100-93325] - Detection of Human NUP96 on HeLa whole cell lysate using NB100-93325. NUP96 was also immunoprecipitated by rabbit anti-NUP96 antibody NB100-93326.")
![Western Blot: NPM1 Antibody [NBP2-27157] - Analysis of Nucleophosmin in human spleen tissue lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse spleen using NBP2-27204. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NPM1-Antibody-Western-Blot-NBP2-27157-img0004.jpg "Western Blot: NPM1 Antibody [NBP2-27157] - Analysis of Nucleophosmin in human spleen tissue lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse spleen using NBP2-27204. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP2-27157] - Analysis of Nucleophosmin in formalin-fixed, paraffin-embedded human liver tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP2-27157-img0003.jpg)
![Western Blot: SA1 Antibody [NB100-298] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.](http://images.novusbio.com/fullsize/SA1-Antibody-Western-Blot-NB100-298-img0003.jpg "Western Blot: SA1 Antibody [NB100-298] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-298] - HeLa cells that were extracted for 5 min at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/SA1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-298-img0002.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
