| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38103-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99648-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97603-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97636-50ul | |||
![Western Blot:[NBP3-28970]-Detection of human and mouse CD1D by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat and from mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD1D antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-28970_rabbit-cd1d-pab-231202511585525.jpg "Western Blot:[NBP3-28970]-Detection of human and mouse CD1D by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat and from mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD1D antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot:[NBP3-28970]-CD1d Antibody](http://images.novusbio.com/fullsize/nbp3-28970_rabbit-cd1d-pab-31120250364041.jpg)
![Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166] - IHC analysis of CHFR in paraffin-embedded Human Brain (Cerebellum) using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CHFR-Antibody-Immunohistochemistry-Paraffin-NB100-1166-img0002.jpg "Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166] - IHC analysis of CHFR in paraffin-embedded Human Brain (Cerebellum) using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: CHFR Antibody [NBP1-52955] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/CHFR-Antibody-Western-Blot-NBP1-52955-img0003.jpg "Western Blot: CHFR Antibody [NBP1-52955] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: CHFR Antibody [NBP1-52955] - Human breast tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/CHFR-Antibody-Immunohistochemistry-Paraffin-NBP1-52955-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: NCAPH Antibody [NBP3-38103] - Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38103_rabbit-ncaph-pab-912202415161866.jpg "Immunocytochemistry/ Immunofluorescence: NCAPH Antibody [NBP3-38103] - Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: NCAPH Antibody [NBP3-38103] - Western blot analysis of various lysates using NCAPH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38103_rabbit-ncaph-pab-91220241418134.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99648] - Immunochemical staining of human PRC1 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunohistochemistry-Paraffin-NBP2-99648-img0002.jpg "Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99648] - Immunochemical staining of human PRC1 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-99648] - Immunofluorescence staining of PRC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99648 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/nbp2-99648_rabbit-polyclonal-prc1-antibody-23520251628112.png)
![Western Blot: NCAPH Antibody [NBP2-97603] - Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP2-97603-img0003.jpg "Western Blot: NCAPH Antibody [NBP2-97603] - Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa")
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP2-97603] - Immunofluorescence staining of NCAPH in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97603 antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97603_rabbit-polyclonal-ncaph-antibody-23520251433150.png)
![Immunohistochemistry-Paraffin: RASSF1 Antibody [NBP2-97636] - Immunochemical staining of human RASSF1 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RASSF1-Antibody-Immunohistochemistry-Paraffin-NBP2-97636-img0002.jpg "Immunohistochemistry-Paraffin: RASSF1 Antibody [NBP2-97636] - Immunochemical staining of human RASSF1 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody [NBP2-97636] - Immunofluorescence staining of RASSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97636 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97636_rabbit-polyclonal-rassf1-antibody-23520251544336.png)
![Immunohistochemistry: Ki67/MKI67 Antibody (PSH0-02) [NBP3-32499] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32499) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32499) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32499_rabbit-ki67-mki67-mab-psh0-02-298202414262672.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (PSH0-02) [NBP3-32499] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32499) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32499) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Ki67/MKI67 Antibody (PSH0-02) [NBP3-32499] - Flow cytometric analysis of A431 cells labeling Ki67/MKI67. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32499, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32499_rabbit-ki67-mki67-mab-psh0-02-29202416372190.jpg)