| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94505-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12876-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98122-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99436-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26216-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25266 | |||
| Azide and BSA Free | NBP2-25266SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47772-0.1mg | |||
| IHC-Prediluted | NBP2-48104 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
![Western Blot: SEC61A Antibody [NBP2-94505] - Analysis of extracts of A-549 cells, using SEC61A .Exposure time: 90s.](http://images.novusbio.com/fullsize/SEC61A-Antibody-Western-Blot-NBP2-94505-img0001.jpg "Western Blot: SEC61A Antibody [NBP2-94505] - Analysis of extracts of A-549 cells, using SEC61A .Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] - Immunofluorescence analysis of HeLa cells using SEC61A Rabbit pAb (A11614) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94505_rabbit-polyclonal-sec61a-antibody-52202519302996.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] - Immunofluorescence analysis of PC-12 cells using SEC61A Rabbit pAb (A11614) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94505_rabbit-polyclonal-sec61a-antibody-52202519294516.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] - Immunofluorescence analysis of NIH/3T3 cells using SEC61A Rabbit pAb (A11614) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94505_rabbit-polyclonal-sec61a-antibody-52202519302918.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Western-Blot-NBP1-04346-img0007.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP1-04346-img0006.jpg)
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). Thesecondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Flow-Cytometry-NBP1-04346-img0008.jpg)
![SDS-Page: Recombinant Human Calreticulin Protein [NBP1-44499] - Calreticulin, 48.7 kDa (421aa), confirmed by MALDI-TOF with a purity of 85% by SDS - PAGE](http://images.novusbio.com/fullsize/Recombinant-Human-Calreticulin-Protein-SDS-Page-NBP1-44499-img0002.jpg "SDS-Page: Recombinant Human Calreticulin Protein [NBP1-44499] - Calreticulin, 48.7 kDa (421aa), confirmed by MALDI-TOF with a purity of 85% by SDS - PAGE")
![Western Blot: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (NBP2-12876). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12876) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-3B9-E6-Western-Blot-NBP2-12876-img0007.jpg "Western Blot: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (NBP2-12876). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12876) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Hsp40 HDJ1 visulaized using, tested on a human keratinocyte line HaCat. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-3B9-E6-Immunohistochemistry-Paraffin-NBP2-12876-img0004.jpg)
![Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-98122-img0002.jpg "Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse intestine with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0004.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0003.jpg)
![Immunoprecipitation: CDC37 Antibody [NBP2-98122] - Mouse CDC37 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-Mouse CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse CDC37 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-98122-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of CDC37 in mouse kidney with NBP2-98122 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-98122_rabbit-polyclonal-cdc37-antibody-23520252135161.png)
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 180 kDa. Observed band size: 180 kDa](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Western-Blot-NBP2-99436-img0006.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 180 kDa. Observed band size: 180 kDa")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in rat kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0005.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0002.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in mouse adrenal gland with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0003.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0004.jpg)
![Immunohistochemistry: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32446) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-298202414333651.jpg "Immunohistochemistry: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32446) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunocytochemistry analysis of HeLa cells labeling HSP90 beta with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-2920241048485.jpg)
![Western blot analysis on different lysates with NBP3-32446 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: Mouse brain tissue lysate (40 µg/Lane) Lane 10: Mouse heart tissue lysate (40 µg/Lane) Lane 11: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32446 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32446_-western-blot-639204590075224668.jpg)
![Immunoprecipitation: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Importin beta/KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32511 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32511 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: Rabbit IgG instead of NBP3-32511 in HeLa cell lysate<br/> Lane 3: NBP3-32511 IP in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32511_rabbit-importin-beta-kpnb1-mab-psh01-60-2682024158263.jpg "Immunoprecipitation: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Importin beta/KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32511 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32511 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: Rabbit IgG instead of NBP3-32511 in HeLa cell lysate<br/> Lane 3: NBP3-32511 IP in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 24 seconds")
![Immunohistochemistry: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling Importin beta/KPNB1 with Rabbit anti-Importin beta/KPNB1 antibody (NBP3-32511) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32511, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32511_rabbit-importin-beta-kpnb1-mab-psh01-60-29820241420963.jpg)
![Immunohistochemistry: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Importin beta/KPNB1 antibody (NBP3-32511) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32511) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32511_rabbit-importin-beta-kpnb1-mab-psh01-60-29820241435256.jpg)
![Western blot analysis on different lysates with NBP3-32511 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: Caco-2 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: PC-12 cell lysate (20 µg/Lane) Lane 10: C6 cell lysate (20 µg/Lane) Lane 11: Mouse spleen tissue lysate (40 µg/Lane) Lane 12: Mouse testis tissue lysate (40 µg/Lane) Lane 13: Rat spleen tissue lysate (40 µg/Lane) Lane 14: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32511 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32511_-western-blot-639204595244826326.jpg)
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-262202415355731.jpg "Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-26220241522415.jpg)
![Western Blot: HSP90 beta Antibody (7G7) [NBP3-26216] - Positive Western Blot detected in: HepG2 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate. All lanes: HSP90 beta Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa Observed band size: 80-90 kDa](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202411485820.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (7G7) [NBP3-26216] - Staining of HepG2 cell with HSP90 beta Antibody (7G7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 495-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-28220241312214.jpg)
![Flow Cytometry: HSP90 beta Antibody (7G7) [NBP3-26216] - Overlay Peak curve showing Hela cells stained with HSP90 beta Antibody (7G7) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202414312789.jpg)
![Western Blot: Noxa Antibody (69N15C9) [NBP2-25251] - Western blot analysis of mouse A) Noxa recombinant protein, B) thymus and C) ovary using Noxa antibody at 1 ug/mL, 5 ug/mL and 5 ug/mL, respectively.](http://images.novusbio.com/fullsize/Noxa-Antibody-69N15C9-Western-Blot-NBP2-25251-img0001.jpg "Western Blot: Noxa Antibody (69N15C9) [NBP2-25251] - Western blot analysis of mouse A) Noxa recombinant protein, B) thymus and C) ovary using Noxa antibody at 1 ug/mL, 5 ug/mL and 5 ug/mL, respectively.")
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (NX2.1/690) [NBP2-44500] - Human Thyroid stained with TTF-1 Monoclonal Antibody (NX2.1/690)](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-NX2-1-690-Immunohistochemistry-Paraffin-NBP2-44500-img0002.jpg "Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (NX2.1/690) [NBP2-44500] - Human Thyroid stained with TTF-1 Monoclonal Antibody (NX2.1/690)")
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (NX2.1/690) [NBP2-44500] - Human Lung Adenocarcinoma stained with TTF-1 Monoclonal Antibody (NX2.1/690)](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-NX2-1-690-Immunohistochemistry-Paraffin-NBP2-44500-img0001.jpg)
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (NX2.1/690) [NBP2-44500] - Rat Lung stained with TTF-1 Monoclonal Antibody (NX2.1/690)](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-NX2-1-690-Immunohistochemistry-Paraffin-NBP2-44500-img0003.jpg)
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)