| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26154-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54741-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21165-25ul | |||
| Azide and BSA Free | NBP3-44301 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54740-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55920-25ul | |||
![Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376] - Western blot analysis of extracts of various cell lines, using GABARAP Rabbit mAb (NBP3-16376) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GABARAP-Antibody-5T6W5-Western-Blot-NBP3-16376-img0001.jpg "Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376] - Western blot analysis of extracts of various cell lines, using GABARAP Rabbit mAb (NBP3-16376) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-28220241144268.jpg "Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa")
![Flow Cytometry: LC3B Antibody (7D11) [NBP3-26154] - Overlay Peak curve showing Hela cells stained with LC3B Antibody (7D11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-282202414183621.jpg)
![Western Blot: ATG16L1 Antibody (S03-4G8) [NBP3-19576] - Western blot detection of ATG16L1 in 3T3 cell lysates using NBP3-19576 (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-S03-4G8-Western-Blot-NBP3-19576-img0001.jpg "Western Blot: ATG16L1 Antibody (S03-4G8) [NBP3-19576] - Western blot detection of ATG16L1 in 3T3 cell lysates using NBP3-19576 (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.")
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-54741] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-54741-img0001.jpg "Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-54741] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.")
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-54740] - Staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-54740-img0001.jpg "Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-54740] - Staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.")
![Immunocytochemistry/Immunofluorescence: TOR/mTOR Antibody [NBP2-55920] - Staining of human cell line HEK 293 shows localization to vesicles.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55920-img0001.jpg "Immunocytochemistry/Immunofluorescence: TOR/mTOR Antibody [NBP2-55920] - Staining of human cell line HEK 293 shows localization to vesicles.")
![Immunoprecipitation:[NBP3-29201]-Detection of human Atg16L1 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Atg16L1 antibody used for IP at 6 µg/mg lysate. Atg16L1 was also immunoprecipitated by rabbit anti-Atg16L1 antibody. For blotting immunoprecipitated Atg16L1, was used at 1 µg/ml. Detection: Chemiluminescence with exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29201_rabbit-atg16l1-pab-23120251252830.jpg "Immunoprecipitation:[NBP3-29201]-Detection of human Atg16L1 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Atg16L1 antibody used for IP at 6 µg/mg lysate. Atg16L1 was also immunoprecipitated by rabbit anti-Atg16L1 antibody. For blotting immunoprecipitated Atg16L1, was used at 1 µg/ml. Detection: Chemiluminescence with exposure time of 3 seconds.")
![Western Blot: TOR/mTOR [p Ser2481] Antibody (9Y5P6) [NBP3-16907] - Western blot analysis of extracts of various cell lines, using TOR/mTOR (NBP3-16907) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/TOR-mTOR-p-Ser2481-Antibody-9Y5P6-Western-Blot-NBP3-16907-img0001.jpg "Western Blot: TOR/mTOR [p Ser2481] Antibody (9Y5P6) [NBP3-16907] - Western blot analysis of extracts of various cell lines, using TOR/mTOR (NBP3-16907) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: ATG2B Antibody [NBP3-13079] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG2B antibody (NBP3-13079) diluted at 1:1000.](http://images.novusbio.com/fullsize/ATG2B-Antibody-Western-Blot-NBP3-13079-img0001.jpg "Western Blot: ATG2B Antibody [NBP3-13079] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG2B antibody (NBP3-13079) diluted at 1:1000.")
![Western Blot: TOR/mTOR [p Ser2481] Antibody [NBP3-13172] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR(phospho Ser2481) antibody (NBP3-13172) diluted at 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2481]-Antibody-Western-Blot-NBP3-13172-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2481] Antibody [NBP3-13172] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR(phospho Ser2481) antibody (NBP3-13172) diluted at 1:1000.")