| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB79672-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82009-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44199 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44488 | |||
| BSA Free | NBP2-52868-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08901 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44489 | |||
| BSA Free | NBP2-14783-25ul | |||
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-10122024140443.jpg "Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177017.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177023.jpg)
![Western Blot: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Western Blot analysis of various lysates using ERK1/2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-31120251744429.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025173186.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)
![Western Blot: RSK1 [p Ser380] Antibody (1024F) [MAB79672] - Analysis of HeLa -/+ PMA cell lysates with 0.25 ug/ml of hRSK (S380) Monoclonal Antibody followed by HAF008. A specific band was detected for hRSK (S380) at approximately90 kDA (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/RSK1-p-Ser380-Antibody-1024F-Western-Blot-MAB79672-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody (1024F) [MAB79672] - Analysis of HeLa -/+ PMA cell lysates with 0.25 ug/ml of hRSK (S380) Monoclonal Antibody followed by HAF008. A specific band was detected for hRSK (S380) at approximately90 kDA (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RSK1 [p Ser380] Antibody (1024F) [MAB79672] - RSK1 [pSer380] was detected in immersion fixed HeLa cells treated with 200 nm of PMA using RSK1 [pSer380] Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm.](http://images.novusbio.com/fullsize/RSK1-p-Ser380-Antibody-1024F-Immunocytochemistry-Immunofluorescence-MAB79672-img0003.jpg)
![Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0001.jpg "Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).")
![<b>Biological Strategies Validation. </b>Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) Antibody](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0003.jpg)
![Immunohistochemistry: JNK1 [p Thr183] Antibody [NB100-82009] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82009](http://images.novusbio.com/fullsize/nb100-82009_rabbit-polyclonal-jnk1-p-thr183-antibody-103202523464547.jpg)
![Western Blot: DMAP1 Antibody (JE55-31) [NBP3-32271] - Western blot analysis of DMAP1 on different lysates with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: F9 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32271) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-278202416285275.jpg "Western Blot: DMAP1 Antibody (JE55-31) [NBP3-32271] - Western blot analysis of DMAP1 on different lysates with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: F9 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32271) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DMAP1 Antibody (JE55-31) [NBP3-32271] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32271) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-298202414262626.jpg)
![Immunocytochemistry/ Immunofluorescence: DMAP1 Antibody (JE55-31) [NBP3-32271] - Immunocytochemistry analysis of HeLa cells labeling DMAP1 with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-292024119235.jpg)
![Flow Cytometry: DMAP1 Antibody (JE55-31) [NBP3-32271] - Flow cytometric analysis of HeLa cells labeling DMAP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32271, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-392024884644.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human testis and endometrium tissues using NBP2-88942 antibody. Corresponding SPAG5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human testis and endometrium tissues using NBP2-88942 antibody. Corresponding SPAG5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Western-Blot-NBP2-88942-img0006.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human endometrium shows very weak cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0001.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human prostate shows no cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0002.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0003.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0004.jpg)
![Western Blot: CD45 Antibody (CL0159) [NBP2-52868] - Western Blot analysis using CD45 antibody (CL0159) [NBP2-52868]. Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate. Observed molecular weight ~200 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Western-Blot-NBP2-52868-img0006.jpg "Western Blot: CD45 Antibody (CL0159) [NBP2-52868] - Western Blot analysis using CD45 antibody (CL0159) [NBP2-52868]. Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate. Observed molecular weight ~200 kDa.")
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human tonsil with CD45 antibody (CL0159) [NBP2-52868] shows strong membranous immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human cortex using CD45 antibody (CL0159) [NBP2-52868] shows strong immunoreactivity in microglia.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-NBP2-52868-img0001.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human liver with CD45 antibody (CL0159) [NBP2-52868] shows strong positivity in Kupffer cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human lung with CD45 antibody (CL0159) [NBP2-52868] shows strong membranous positivity in macrophages.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human small intestine with CD45 antibody (CL0159) [NBP2-52868] shows strong immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0004.jpg)
![Western Blot: CD45RA Antibody (K4B5) [NBP3-07159] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (K4B5)](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Western-Blot-NBP3-07159-img0004.jpg "Western Blot: CD45RA Antibody (K4B5) [NBP3-07159] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (K4B5)")
![Immunohistochemistry-Paraffin: CD45RA Antibody (K4B5) [NBP3-07159] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Mouse Monoclonal Antibody (K4B5).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Immunohistochemistry-Paraffin-NBP3-07159-img0003.jpg)
![Flow Cytometry: CD45RA Antibody (K4B5) [NBP3-07159] - Flow Cytometric Analysis of Jurkat cells using CD45RA Mouse Monoclonal Antibody (K4B5) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Flow-Cytometry-NBP3-07159-img0001.jpg)
![Immunohistochemistry-Paraffin: CD45RA Antibody (K4B5) [NBP3-07159] - Formalin-fixed, paraffin-embedded human Spleen stained with CD45RA Mouse Monoclonal Antibody (K4B5).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Immunohistochemistry-Paraffin-NBP3-07159-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Analysis in human tonsil and skeletal muscle tissues using NBP2-14783 antibody. Corresponding PTPRC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411351..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Analysis in human tonsil and skeletal muscle tissues using NBP2-14783 antibody. Corresponding PTPRC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD45 Antibody (CL0160) [NBP2-14783] - Western Blot analysis using CD45 antibody (CL0160) [NBP2-14783]. Lane 1: Marker [kDa]. Lane 2: Human cell line Jurkat. Lane 3: Human cell line MCF-7. Observed molecular weight ~230 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0160-Western-Blot-NBP2-14783-img0007.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] - Staining of human tonsil using CD45 antibody (CL0160) [NBP2-14783] shows strong positivity in a majority of lymphoid cells](http://images.novusbio.com/fullsize/CD45-Antibody-CL0160-Immunohistochemistry-Paraffin-NBP2-14783-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411445..jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human duodenum shows moderate membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411549..jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411643..jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Western-Blot-NBP3-13678-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - JNK1 (phospho Thr183/Tyr185) antibody [HL1008] detects JNK1 (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. JNK1 (phospho Thr183/Tyr185) stained by JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Immunohistochemistry-Paraffin-NBP3-13678-img0002.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-9102024204127.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) Raw264.7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020242013406.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) PC-12 whole cell extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020241959351.jpg)
![Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-western-blot-261120251837507.jpg)
![Western Blot: MAP4K6 Antibody [NBP1-22990] - Whole cell lysate from HeLa and mouse NIH3T3 cells at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/MAP4K6-Antibody-Western-Blot-NBP1-22990-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MAP4K6 Antibody [NBP1-22990] - Whole cell lysate from HeLa and mouse NIH3T3 cells at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.")