| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33104 | |||
| IHC-Prediluted | NBP2-44839 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44853 | |||
| Azide and BSA Free | NBP2-34527-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38532-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87790-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11267-0.025ml | |||
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)Please notice that NBP3-45157 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45158.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)Please notice that NBP3-45158 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45157. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Tau (p Thr181) antibody [HL2497] detects Tau (p Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse brain.Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2497] (GTX638855) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg)
![Western Blot: AMPK alpha 2 Antibody [NB100-238] - Detection of Human and Mouse AMPK alpha 2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-AMPK alpha 2 antibody NB100-238 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-Western-Blot-NB100-238-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: AMPK alpha 2 Antibody [NB100-238] - Detection of Human and Mouse AMPK alpha 2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-AMPK alpha 2 antibody NB100-238 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405] - Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine. The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.Lane A: Antibody dilution 1:1000Lane B: Negative control (only secondary antibody)](http://images.novusbio.com/fullsize/Phosphoserine-Antibody-PSR-45-Western-Blot-NB600-405-img0003.jpg "Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405] - Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine. The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.Lane A: Antibody dilution 1:1000Lane B: Negative control (only secondary antibody)")
![Western Blot: MEK2 Antibody (BLR079G) [NBP2-78800] - Antibody: Rabbit anti-MEK2 recombinant monoclonal [BLR079G] (NBP2-78800) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).](http://images.novusbio.com/fullsize/MEK2-Antibody-BLR079G-Western-Blot-NBP2-78800-img0004.jpg "Western Blot: MEK2 Antibody (BLR079G) [NBP2-78800] - Antibody: Rabbit anti-MEK2 recombinant monoclonal [BLR079G] (NBP2-78800) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).")
![Immunocytochemistry: MEK2 Antibody (BLR079G) [NBP2-78800] - Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (NBP2-78800). Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/MEK2-Antibody-BLR079G-Immunocytochemistry-NBP2-78800-img0003.jpg)
![Immunohistochemistry-Paraffin: MEK2 Antibody (BLR079G) [NBP2-78800] - Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (NBP2-78800). Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/MEK2-Antibody-BLR079G-Immunohistochemistry-NBP2-78800-img0002.jpg)
![Immunohistochemistry-Paraffin: MEK2 Antibody (BLR079G) [NBP2-78800] - Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (NBP2-78800). Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/MEK2-Antibody-BLR079G-Immunohistochemistry-NBP2-78800-img0001.jpg)
![Immunoprecipitation: MEK2 Antibody (BLR079G) [NBP2-78800] - Antibody: Rabbit anti-MEK2 recombinant monoclonal [BLR079G] (NBP2-78800).](http://images.novusbio.com/fullsize/MEK2-Antibody-BLR079G-Immunoprecipitation-NBP2-78800-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631] - Staining of human tonsil](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Immunohistochemistry-Paraffin-NBP2-29631-img0004.jpg "Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631] - Staining of human tonsil")
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD45RO Antibody (UCHL-1) [NBP2-29631] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD45 mRNA (ACD RNAScope probe, catalog # 601991; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-29631) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Dual-RNAscope-ISH-IHC-NBP2-29631-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD45RO Antibody (UCHL-1) [NBP2-29631] - Immunofluorescence Analysis of human tonsil FFPE section stained with CD45RO antibody (UCHL-1)and goat anti-mouse HRP, detected using CF488A tyramide in Tyramide Amplification Buffer Plus.](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Immunocytochemistry-Immunofluorescence-NBP2-29631-img0008.jpg)
![Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Antibody (UCHL-1) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Flow-Cytometry-NBP2-29631-img0007.jpg)
![Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631] - CD45RO expression by lymphocyte gated population of PBMC: PBMC were stained either with purified CD45RO antibody (0.5 ug/10^6 cells) or isotype control following surface staining protocol. PE-conjugated goat anti-mouse IgG, secondary antibody used to identify the positive staining on PPI negative lymphocyte gated population.](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-Flow-Cytometry-NBP2-29631-img0002.jpg)
![Formalin-fixed, paraffin-embedded human tonsil stained with CD45 antibody (2B11) [NBP2-32934].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-32934-img0002.jpg "Formalin-fixed, paraffin-embedded human tonsil stained with CD45 antibody (2B11) [NBP2-32934].")
![Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD45 antibody (2B11) [NBP2-32934].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-32934-img0001.jpg)
![Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/ERK1-[p-Thr202--p-Tyr204]-Antibody-Western-Blot-NB500-141-img0003.jpg "Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.](http://images.novusbio.com/fullsize/ERK1-[p-Thr202--p-Tyr204]-Antibody-Immunohistochemistry-NB500-141-img0004.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67373-img0006.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded human kidney tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0004.jpg)
![Western Blot: AMPK alpha 2 Antibody [NBP2-38532] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-Western-Blot-NBP2-38532-img0002.jpg "Western Blot: AMPK alpha 2 Antibody [NBP2-38532] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 2 Antibody [NBP2-38532] - Staining of human cell line HEK 293 shows localization to nuclear speckles & the Golgi apparatus.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38532-img0003.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody [NBP2-38532] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38532-img0007.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody [NBP2-38532] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38532-img0005.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody [NBP2-38532] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38532-img0006.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody [NBP2-38532] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38532-img0008.jpg)
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human small intestine shows no cytoplasmic positivity as expected.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0006.jpg "Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human small intestine shows no cytoplasmic positivity as expected.")
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemistry analysis in human cerebral cortex and small intestine tissues using NBP1-81264 antibody. Corresponding MAP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0002.jpg)
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0003.jpg)
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity..](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0004.jpg)
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human pancreas shows moderate to strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0005.jpg)
![Immunofluorescence: MAP2 Antibody [NBP1-81264] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunofluorescence-NBP1-81264-img0001.jpg)
![Western Blot: MEK1 Antibody [NBP1-87790] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/MEK1-Antibody-Western-Blot-NBP1-87790-img0009.jpg "Western Blot: MEK1 Antibody [NBP1-87790] - Analysis in human cell line U-87 MG.")
![Immunocytochemistry/Immunofluorescence: MEK1 Antibody [NBP1-87790] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87790-img0007.jpg)
![Immunohistochemistry: MEK1 Antibody [NBP1-87790] - Correlation between MEK1 expression and survival after platinum treatment in EOC patients. Representative images of immunohistochemistry, low and high expression of MEK1 at low and high magnifications. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-837), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunohistochemistry-NBP1-87790-img0008.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody [NBP1-87790] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-87790-img0001.jpg)
![Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Western-Blot-NB120-11267-img0003.jpg "Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HM-2) [NB120-11267] - B35 cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Immunocytochemistry-Immunofluorescence-NB120-11267-img0002.jpg)