| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48480SS | |||
| Azide and BSA Free | NBP2-80552 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85740-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25243 | |||
| BSA Free | NBP2-25237SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71214 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37271-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37274-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52556-0.025mg | |||
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution). Image from verified customer review.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Immunohistochemistry-Paraffin-NBP2-48480-img0006.jpg "Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution). Image from verified customer review.")
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cytometry-NBP2-48480-img0005.jpg)
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0001.jpg)
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0002.jpg)
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for human aortic valve endothelial cells. Image from a verified customer review.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85740-img0024.jpg "Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for human aortic valve endothelial cells. Image from a verified customer review.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human liver shows strong membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0027.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human aortic valve staining for CD73. Image from verified customer review.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-NBP1-85740-img0019.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for pig aortic valve. Image from verified customer review.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-NBP1-85740-img0020.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human duodenum shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0025.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0026.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Analysis of CD73 in a partial recombinant protein using CD73 antibody at 1 ug/ml. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-45M4F9-Western-Blot-NBP2-25237-img0004.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Formalin-fixed, paraffin-embedded human breast stained with CD73 antibody (20 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-45M4F9-Immunohistochemistry-Paraffin-NBP2-25237-img0003.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135] - Flow cytometric detection of human CD73 on Huvec (mAb 4G4, Cat.# NBP1-60135). Black, red and blue line represents cells only, isotype control and 4G4 (4 ug/ml, respectively).](http://images.novusbio.com/fullsize/CD73-Antibody-4G4-Flow-Cytometry-NBP1-60135-img0001.jpg "Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135] - Flow cytometric detection of human CD73 on Huvec (mAb 4G4, Cat.# NBP1-60135). Black, red and blue line represents cells only, isotype control and 4G4 (4 ug/ml, respectively).")
![Western Blot: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 5'-Nucleotidase/CD73.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Western-Blot-NBP2-46259-img0010.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 5'-Nucleotidase/CD73.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0009.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0001.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0002.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0003.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0004.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0005.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0006.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0007.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0008.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP2-15815-img0005.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - CD73 antibody detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: CD73 stained by CD73 antibody diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP2-15815-img0006.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP2-15815-img0004.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - J5 xenograft, using CD73 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP2-15815-img0003.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5'-Nucleotidase/CD73 antibody [N1N3] (NBP2-15815) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15815_rabbit-polyclonal-5-nucleotidase-cd73-antibody-910202420104432.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-15815_rabbit-polyclonal-5-nucleotidase-cd73-antibody-1610202419405713.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 5'-Nucleotidase/CD73.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Western-Blot-NBP2-71214-img0001.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 5'-Nucleotidase/CD73.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0009.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0002.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0003.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0004.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0005.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0006.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0007.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0008.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-OTI1G2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71214-img0010.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0007.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Flow-Cytometry-NBP2-37271-img0005.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0006.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-ELISA-NBP2-37271-img0001.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of esophagus cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0006.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against human NT5E (AA: ) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of bladder cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0005.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-ELISA-NBP2-37274-img0001.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Western-Blot-NBP3-14679-img0003.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG Substrate DAB.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunocytochemistry-NBP3-14679-img0002.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of human tonsil. Antibody: rabbit anti-CD73 recombinant monoclonal antibody used at 1:250. secondary: HRP- conjugated goat anti-rabbit igG](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunohistochemistry-NBP3-14679-img0004.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of human head and neck squamous cell carcinoma. Rabbit anti-CD73 recombinant monoclonal antibody used at 1:500. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunohistochemistry-NBP3-14679-img0001.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0003.jpg "Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0007.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0006.jpg)
![ELISA: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-ELISA-NBP2-52556-img0005.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-278202416285219.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-298202414304184.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunocytochemistry analysis of U-87 MG cells labeling 5'-Nucleotidase/CD73 with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-2920241192315.jpg)