| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33606-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33685-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89827-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79944 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93766-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97726-50ul | |||
![Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-32695-img0017.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-32695] - Antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32695-img0001.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-32695] - A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-32695-img0013.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-32695-img0015.jpg)
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP1-32695] - IP samples: HeLa whole cell extract A. 40 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of YWHAE protein by 4 ug 14-3-3 epsilon antibody [N1C3] (NBP1-32695) 12 % SDS-PAGE The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] (NBP1-32695) diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP1-32695-img0012.jpg)
![Western Blot: PCK2 Antibody [NBP2-33606] - Analysis using Anti-PCK2 antibody NBP2-33606 (A) shows similar pattern to independent antibody NBP2-33685 (B).](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-33606-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PCK2 Antibody [NBP2-33606] - Analysis using Anti-PCK2 antibody NBP2-33606 (A) shows similar pattern to independent antibody NBP2-33685 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP2-33606] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33606-img0006.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human liver.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0015.jpg)
![Immunohistochemistry: PCK2 Antibody [NBP2-33606] - Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-NBP2-33606-img0007.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining in human duodenum and pancreas tissues using anti-PCK2 antibody. Corresponding PCK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human cerebral cortex, colon, kidney and liver using Anti-PCK2 antibody NBP2-33606 (A) shows similar protein distribution across tissues to independent antibody NBP2-33685 (B).](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0010.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human colon.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0012.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0013.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human kidney.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0014.jpg)
![Western Blot: PCK2 Antibody [NBP2-33685] - Analysis using Anti-PCK2 antibody NBP2-33685 (A) shows similar pattern to independent antibody NBP2-33606 (B).](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-33685-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PCK2 Antibody [NBP2-33685] - Analysis using Anti-PCK2 antibody NBP2-33685 (A) shows similar pattern to independent antibody NBP2-33606 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP2-33685] - Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33685-img0003.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human liver.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0012.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human duodenum shows high expression.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0004.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining in human duodenum and skeletal muscle tissues using anti-PCK2 antibody. Corresponding PCK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human cerebral cortex, colon, kidney and liver using Anti-PCK2 antibody NBP2-33685 (A) shows similar protein distribution across tissues to independent antibody NBP2-33606 (B).](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0007.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human colon.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0009.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0010.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human kidney.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-89827] - Staining 14-3-3 epsilon in methanol-fixed, rat primary motor neurons using anti-14-3-3 epsilon antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89827-img0010.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-89827] - Staining 14-3-3 epsilon in methanol-fixed, rat primary motor neurons using anti-14-3-3 epsilon antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer review.")
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-89827] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-89827-img0009.jpg)
![Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NB100-2422-img0002.jpg "Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NB100-2422] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2422-img0003.jpg)
![Flow Cytometry: PCK2 Antibody [NB100-2422] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PCK2-Antibody-Flow-Cytometry-NB100-2422-img0004.jpg)
![Western Blot: PCK2 Antibody [NB100-2422] - Staining of A431 (A), HEK293 (B) with antibody at 2 ug/mL and HepG2 (C) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NB100-2422-img0001.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0006.jpg "Western Blot: PCK2 Antibody [NBP2-19729] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-19729] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-19729-img0004.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - PCK2 antibody detects PCK2 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract7. 5 % SDS-PAGEPCK2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0001.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - PCK2 antibody detects PCK2 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract7. 5 % SDS-PAGEPCK2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0002.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0005.jpg "Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP1-31241] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31241-img0006.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP1-31241] - OVCAR3 xenograft, using PCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-31241-img0002.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0003.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0004.jpg)
![Western Blot: PCK2 Antibody (10L10J8) [NBP3-16418] - Western blot analysis of extracts of various cell lines, using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PCK2-Antibody-10L10J8-Western-Blot-NBP3-16418-img0002.jpg "Western Blot: PCK2 Antibody (10L10J8) [NBP3-16418] - Western blot analysis of extracts of various cell lines, using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody (10L10J8) [NBP3-16418] - Immunofluorescence analysis of HeLa cells using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PCK2-Antibody-10L10J8-Immunocytochemistry-Immunofluorescence-NBP3-16418-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PCK2 Antibody (10L10J8) [NBP3-16418] - Confocal imaging of C2C12 cells using [KO Validated] PCK2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16418_rabbit-pck2-mab-10l10j8-5220251242570.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PCK2 Antibody (10L10J8) [NBP3-16418] - Western blot analysis of lysates from wild type(WT) and PCK2 knockout (KO) 293T cells, using [KO Validated] PCK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16418_rabbit-pck2-mab-10l10j8-522025124251.jpg)
![Immunocytochemistry/ Immunofluorescence: PCK2 Antibody (10L10J8) [NBP3-16418] - Confocal imaging of HeLa cells using [KO Validated] PCK2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16418_rabbit-pck2-mab-10l10j8-5220251255515.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-33554-img0005.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33554-img0002.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554] - Paraffin-embedded MDA-MB231 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-33554-img0003.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-33554-img0004.jpg)
![Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3 epsilon Antibody (CPTC-YWHAE-1).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Western-Blot-NBP2-79810-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3 epsilon Antibody (CPTC-YWHAE-1).")
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Formalin-fixed, paraffin-embedded human Testis stained with 14-3-3 epsilon Antibody (CPTC-YWHAE-1).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Immunohistochemistry-Paraffin-NBP2-79810-img0001.jpg)
![Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. 14-3-3 epsilon Antibody (NBP2-79810) primary antibody at 1:1000, 4C, overnight. Wb image submitted by a verified customer review.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Western-Blot-NBP2-79810-img0004.jpg)
![Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Analysis of Protein Array containing more than 19,000 full-length human proteins using 14-3-3 epsilon Antibody (CPTC-YWHAE-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Protein-Array-NBP2-79810-img0002.jpg)
![Western Blot: MTCH1 Antibody [NBP2-93766] - Analysis of extracts of various cell lines, using MTCH1 .Exposure time: 30s.](http://images.novusbio.com/fullsize/MTCH1-Antibody-Western-Blot-NBP2-93766-img0002.jpg "Western Blot: MTCH1 Antibody [NBP2-93766] - Analysis of extracts of various cell lines, using MTCH1 .Exposure time: 30s.")
![Immunohistochemistry-Paraffin: MTCH1 Antibody [NBP2-93766] - Paraffin-embedded human breast cancer using MTCH1 .](http://images.novusbio.com/fullsize/MTCH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93766-img0001.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-97726-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-YWHAE rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-YWHAE rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :30 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-97726-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-97726] - Immunofluorescence staining of 14-3-3 epsilon in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97726 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97726_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511545662.png)