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![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0018.jpg "Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in frozen sections of rat glioblastoma tissue. Image courtsey of anonymous customer review.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48308-img0007.jpg)
![Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Staining of PKM2 in mouse liver tissue.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunohistochemistry-NBP1-48308-img0009.jpg)
![Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on Neuro2a cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48308-img0019.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of HeLa lysates using NBP1-48308. Image courtesy of Gregg Semenza, (PMID: 21620138).](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0010.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in MCF7 whole cell lysates.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0012.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression of PKM1 and PKM2 in clinical colorectal cancer samples. The protein expression of PKM1 and PKM2 in clinical specimens of cancer tumor (T) and the adjacent normal tissues (N) is shown. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0016.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in various cancer cell lines, control cell lines, and cells in primary culture. PKM1 and PKM2 were detected by Western blotting under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2a. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in HeLa cells.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48308-img0008.jpg)
![Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Immunohistochemical staining of normal colon tissue adjacent to tumor tissue of case 10. Results of staining with anti-PKM2 is shown. The boxed regions are enlarged in the images below. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25721733/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2 Antibody - BSA Free-Immunohistochemistry-NBP1-48308-img0020.jpg)
![Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on HeLa cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48308-img0014.jpg)
![Simple Western: PKM2 Antibody - BSA Free [NBP1-48308] - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Simple-Western-NBP1-48308-img0013.jpg)
![Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0012.jpg "Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody [NBP1-82487] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82487-img0009.jpg)
![Immunohistochemistry-Paraffin: PKM2 Antibody [NBP1-82487] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunohistochemistry-Paraffin-NBP1-82487-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0011.jpg)
![Western Blot: PKM2 Antibody [NBP2-19852] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP2-19852-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PKM2 Antibody [NBP2-19852] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: PKM2 Antibody [NBP2-19852] - antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Pyruvate Kinase (muscle) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunohistochemistry-Paraffin-NBP2-19852-img0006.jpg)
![Western Blot: PKM2 Antibody [NBP2-19852] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP2-19852-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: PKM2 Antibody [NBP2-19852] - PKM2 antibody detects PKM2 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PKM2 protein stained by PKM2 antibody (NBP2-19852) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19852_rabbit-polyclonal-pkm2-antibody-910202419593528.jpg)
![Immunohistochemistry-Paraffin: PKM2 Antibody [NBP2-19852] - PKM2 antibody detects PKM2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.PKM2 stained by PKM2 antibody (NBP2-19852) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19852_rabbit-polyclonal-pkm2-antibody-91020242014694.jpg)
![Western Blot: PKM2 Antibody [NBP2-19852] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody (NBP2-19852) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-19852_rabbit-polyclonal-pkm2-antibody-91020242011489.jpg)
![Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Western-Blot-NBP3-15230-img0005.jpg "Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (7B2) [NBP3-15230] - Immunofluorescence staining of Hela Cells with at PKM2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunocytochemistry-Immunofluorescence-NBP3-15230-img0002.jpg)
![Immunohistochemistry: PKM2 Antibody (7B2) [NBP3-15230] - IHC image of PKM2 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunohistochemistry-NBP3-15230-img0004.jpg)
![Flow Cytometry: PKM2 Antibody (7B2) [NBP3-15230] - Overlay histogram showing HepG2 cells stained with PKM2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Flow-Cytometry-NBP3-15230-img0001.jpg)
![Immunoprecipitation: PKM2 Antibody (7B2) [NBP3-15230] - Immunoprecipitating PKM2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PKM2 antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PKM2 antibody (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunoprecipitation-NBP3-15230-img0003.jpg)
![Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Western-Blot-NBP3-13632-img0002.jpg "Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (GT1810) [NBP3-13632] - PKM2 antibody [GT1810] detects PKM2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Immunocytochemistry-Immunofluorescence-NBP3-13632-img0003.jpg)
![Immunohistochemistry-Paraffin: PKM2 Antibody (GT1810) [NBP3-13632] - Human lung cancer. PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Immunohistochemistry-Paraffin-NBP3-13632-img0004.jpg)
![Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Western-Blot-NBP3-13632-img0001.jpg)
![Western Blot: PKM2 Antibody (SR2031) [NBP3-21688] - Western blot analysis using NBP3-21688 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21688_rabbit-pkm2-mab-sr2031-5720231558487.jpg "Western Blot: PKM2 Antibody (SR2031) [NBP3-21688] - Western blot analysis using NBP3-21688 in HeLa cell lysate.")
![Immunohistochemistry: PKM2 Antibody (SR2031) [NBP3-21688] - Immunohistochemical analysis of paraffin-embedded human cervix cancer, using NBP3-21688.](http://images.novusbio.com/fullsize/nbp3-21688_rabbit-pkm2-mab-sr2031-57202317215152.jpg)
![Western Blot: PKM2 Antibody (1B10) [NBP2-42686] - Fig 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Western-Blot-NBP2-42686-img0003.jpg "Western Blot: PKM2 Antibody (1B10) [NBP2-42686] - Fig 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Immunocytochemistry-Immunofluorescence-NBP2-42686-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Immunocytochemistry-Immunofluorescence-NBP2-42686-img0004.jpg)
![Immunohistochemistry-Paraffin: Carboxypeptidase B2 Antibody [NBP1-85469] - Staining of human lymph node shows moderate cytoplasmic positivity in reaction center cells.](http://images.novusbio.com/fullsize/Carboxypeptidase-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-85469-img0002.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase B2 Antibody [NBP1-85469] - Staining of human lymph node shows moderate cytoplasmic positivity in reaction center cells.")
![ELISA: Human PKM2 ELISA Kit (Colorimetric) [NBP3-18036] - Samples were spiked with high concentrations of Human PKM2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18036_human-pkm2-elisa-kit-colorimetric-13220241625401.jpg "ELISA: Human PKM2 ELISA Kit (Colorimetric) [NBP3-18036] - Samples were spiked with high concentrations of Human PKM2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human PKM2 ELISA Kit (Colorimetric) [NBP3-18036] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18036_human-pkm2-elisa-kit-colorimetric-4320249124848.jpg)
![ELISA: Rat Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) [NBP3-06963] - Samples were spiked with high concentrations of Rat Carboxypeptidase B2/CPB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06963_rat-carboxypeptidase-b2-cpb2-elisa-kit-colorimetric-13220241685326.jpg "ELISA: Rat Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) [NBP3-06963] - Samples were spiked with high concentrations of Rat Carboxypeptidase B2/CPB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) [NBP3-06963] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06963_rat-carboxypeptidase-b2-cpb2-elisa-kit-colorimetric-4320248574352.jpg)