| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13629-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15122-25ul | |||
| Azide and BSA Free | NBP3-44184 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21199-25ul | |||
| Azide and BSA Free | NBP3-44138 | |||
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Western Blot: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Analysis using Anti-FASN antibody NBP2-49213 (A) shows similar pattern to independent antibody NBP1-84733 (B).](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NBP2-49213-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Analysis using Anti-FASN antibody NBP2-49213 (A) shows similar pattern to independent antibody NBP1-84733 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining in human breast and skeletal muscle tissues using anti-FASN antibody. Corresponding FASN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP2-49213-img0003.jpg)
![Immunohistochemistry: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-NBP2-49213-img0001.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP2-49213-img0002.jpg)
![Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody [NBP3-14735] - Tissue lysate (1.0 mg per IPreaction; 20% of IP loaded) from mouse liver cells preparedusing NETN lysis buffer. Antibody: Rabbit anti-Arginase 1recombinant monoclonal antibody used at 20 ul/mg lysate. For blottingimmunoprecipitated Arginase 1, was used at1:1000. Chemiluminescence with an exposure time of 30seconds.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunoprecipitation-NBP3-14735-img0001.jpg "Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody [NBP3-14735] - Tissue lysate (1.0 mg per IPreaction; 20% of IP loaded) from mouse liver cells preparedusing NETN lysis buffer. Antibody: Rabbit anti-Arginase 1recombinant monoclonal antibody used at 20 ul/mg lysate. For blottingimmunoprecipitated Arginase 1, was used at1:1000. Chemiluminescence with an exposure time of 30seconds.")
![Lysate from from H-4-II-E, NIH 3T3, mouse liver, TCMK-1,and AML12 cells. Antibody: Rabbit anti-Arginase-1recombinant monoclonal antibody [BLR161J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with anexposure time of 30 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F]](http://images.novusbio.com/fullsize/nbp3-14735_rabbit-arginase-1-arg1-liver-arginase-mab-western-blot-2472025113438..png)
![Rabbit anti-Arginase 1 recombinant monoclonal antibody [BLR161J]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14735_rabbit-arginase-1-arg1-liver-arginase-mab-immunohistochemistry-paraffin-2472025113711..png)
![Western Blot: MVD Antibody [NBP2-13629] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/MVD-Antibody-Western-Blot-NBP2-13629-img0002.jpg "Western Blot: MVD Antibody [NBP2-13629] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: MVD Antibody [NBP2-13629] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/MVD-Antibody-Immunohistochemistry-Paraffin-NBP2-13629-img0001.jpg)
![Western Blot: MVD Antibody [NBP2-13629] - Analysis in human cell line MCF-7 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/MVD-Antibody-Western-Blot-NBP2-13629-img0003.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (CL11661) [NBP3-15122] - Staining of human spleen shows strong granular cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-CL11661-Immunohistochemistry-Paraffin-NBP3-15122-img0004.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (CL11661) [NBP3-15122] - Staining of human spleen shows strong granular cytoplasmic positivity in cells in red pulp.")
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (CL11661) [NBP3-15122] - Staining of human fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-CL11661-Immunohistochemistry-Paraffin-NBP3-15122-img0001.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (CL11661) [NBP3-15122] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-CL11661-Immunohistochemistry-Paraffin-NBP3-15122-img0002.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (CL11661) [NBP3-15122] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-CL11661-Immunohistochemistry-Paraffin-NBP3-15122-img0003.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (CL11661) [NBP3-15122] - Analysis in human spleen and skeletal muscle tissues using NBP3-15122 antibody. Corresponding HMOX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-CL11661-Immunohistochemistry-Paraffin-NBP3-15122-img0005.jpg)
![Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-278202415295526.jpg "Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-iNOS antibody (NBP3-32473) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32473) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-298202414304151.jpg)
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunocytochemistry analysis of RAW264.7 cells treated with or without 1μg/mL LPS for 6 hours labeling iNOS with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-29202410334018.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0004.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of Jurkat cells with HO-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Flow-Cytometry-NBP2-67382-img0001.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded human liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0002.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded human spleen tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0003.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Western blot analysis of HO-1/HMOX1/HSP32 on different lysates with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32420) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-27820241524263.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Western blot analysis of HO-1/HMOX1/HSP32 on different lysates with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32420) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32420) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-298202414262647.jpg)
![Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Immunocytochemistry analysis of A549 cells labeling HO-1/HMOX1/HSP32 with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-29202410484883.jpg)
![Western Blot: SEC14L2 Antibody [NBP2-15112] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Western-Blot-NBP2-15112-img0002.jpg "Western Blot: SEC14L2 Antibody [NBP2-15112] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-15112] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-15112-img0001.jpg)
![SEC14L2 antibody [N1C1] detects SEC14L2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SEC14L2 protein stained by SEC14L2 antibody [N1C1] (NBP2-15112) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15112_rabbit-polyclonal-sec14l2-antibody-immunocytochemistry-immunofluorescence-261120251849136.jpg)
![Immunohistochemistry-Paraffin: P2Y2 Antibody [NBP3-12229] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and hematoxyllin QS (blue) counterstrain. 20x magnification on leica DM4000. FFPE section](http://images.novusbio.com/fullsize/P2Y2-Antibody-Immunohistochemistry-Paraffin-NBP3-12229-img0001.jpg "Immunohistochemistry-Paraffin: P2Y2 Antibody [NBP3-12229] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and hematoxyllin QS (blue) counterstrain. 20x magnification on leica DM4000. FFPE section")
![Western Blot: 15-Lipoxygenase 1 Antibody [H00000246-D01P] - Analysis of ALOX15 expression in mouse spleen.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-Western-Blot-H00000246-D01P-img0003.jpg "Western Blot: 15-Lipoxygenase 1 Antibody [H00000246-D01P] - Analysis of ALOX15 expression in mouse spleen.")
![Western Blot: 15-Lipoxygenase 1 Antibody [H00000246-D01P] - Analysis of ALOX15 expression in transfected 293T cell line by ALOX15 polyclonal antibody.Lane 1: ALOX15 transfected lysate(74.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-Western-Blot-H00000246-D01P-img0004.jpg)