| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35289-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90108-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06497-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99999-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89646-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88804-25ul | |||
![Western Blot: ECH1 Antibody [NBP3-35289] - Western blot analysis of lysates from Mouse heart using ECH1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35289_rabbit-ech1-pab-10122024121058.jpg "Western Blot: ECH1 Antibody [NBP3-35289] - Western blot analysis of lysates from Mouse heart using ECH1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: ECH1 Antibody [NBP3-35289] - Western Blot analysis of lysates from Mouse heart using ECH1 Rabbit pAbat 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35289_rabbit-ech1-pab-31120251704762.jpg)
![Western Blot: ECH1 Antibody [NBP3-35289] - Western Blot analysis of various lysates using ECH1 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35289_rabbit-ech1-pab-3112025173126.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Western blot analysis of Carbonic Anhydrase II/CA2 on different lysates with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32105) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-278202415242692.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Western blot analysis of Carbonic Anhydrase II/CA2 on different lysates with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32105) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32105) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29820241420924.jpg)
![Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunocytochemistry analysis of THP-1 cells labeling Carbonic Anhydrase II/CA2 with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29202410334053.jpg)
![Immunohistochemistry-Paraffin: PHPT1 Antibody (001) [NBP2-90108] - Immunochemical staining of human PHPT1 in human skeletal muscle with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PHPT1-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90108-img0001.jpg "Immunohistochemistry-Paraffin: PHPT1 Antibody (001) [NBP2-90108] - Immunochemical staining of human PHPT1 in human skeletal muscle with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections).")
![<b>Biological Strategies Validation. </b>Flow Cytometry: PHPT1 Antibody (001) [NBP2-90108] - Flow cytometric analysis of Human PHPT1 expression on THP1 cells . The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PHPT1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/PHPT1-Antibody-001-Flow-Cytometry-NBP2-90108-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PHPT1 Antibody (001) [NBP2-90108] - Immunofluorescence staining of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90108 antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90108_rabbit-monoclonal-phpt1-antibody-001-23520251144445.png)
![Western Blot: CYB5R3 Antibody [NBP3-06497] - Lane A: A431 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: U87MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa](http://images.novusbio.com/fullsize/CYB5R3-Antibody-Western-Blot-NBP3-06497-img0001.jpg "Western Blot: CYB5R3 Antibody [NBP3-06497] - Lane A: A431 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: U87MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa")
![Immunohistochemistry-Paraffin: CYB5R3 Antibody [NBP3-06497] - Staining of human CYB5R3 in human testis with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-Immunohistochemistry-Paraffin-NBP3-06497-img0002.jpg)
![Immunoprecipitation: CYB5R3 Antibody [NBP3-06497] - Lane A:0.5 mg A431 HepG2 HeLa U87MG Whole Cell Lysate4 uL anti-CYB5R3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CYB5R3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa](http://images.novusbio.com/fullsize/CYB5R3-Antibody-Immunoprecipitation-NBP3-06497-img0003.jpg)
![Western Blot: PHPT1 Antibody [NBP2-99999] - Lane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa](http://images.novusbio.com/fullsize/PHPT1-Antibody-Western-Blot-NBP2-99999-img0005.jpg "Western Blot: PHPT1 Antibody [NBP2-99999] - Lane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa")
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-99999] - Staining of human PHPT1 in human skeletal muscle with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP2-99999-img0003.jpg)
![Immunoprecipitation: PHPT1 Antibody [NBP2-99999] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg THP-1 Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PHPT1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PHPT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunoprecipitation-NBP2-99999-img0004.jpg)
![Western Blot: Thioredoxin Reductase 1/TRXR1 Antibody [NBP1-33210] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Thioredoxin-Reductase-1-TRXR1-Antibody-Western-Blot-NBP1-33210-img0008.jpg "Western Blot: Thioredoxin Reductase 1/TRXR1 Antibody [NBP1-33210] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody [NBP1-33210] - Human hepatoma, dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Thioredoxin-Reductase-1-TRXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-33210-img0009.jpg)
![Western Blot: Thioredoxin Reductase 1/TRXR1 Antibody [NBP1-33210] - Mouse liver lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Thioredoxin-Reductase-1-TRXR1-Antibody-Western-Blot-NBP1-33210-img0007.jpg)
![Immunohistochemistry-Paraffin: MTR Antibody [NBP3-41296] - DAB staining on IHC-P; Sample: Human Pancreas Tissue; Primary Ab: 30ug/ml NBP3-41296; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41296_rabbit-mtr-pab-612202421304386.jpg "Immunohistochemistry-Paraffin: MTR Antibody [NBP3-41296] - DAB staining on IHC-P; Sample: Human Pancreas Tissue; Primary Ab: 30ug/ml NBP3-41296; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody")
![Western Blot: MTR Antibody [NBP3-41296] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-41296_rabbit-mtr-pab-61220242055420.jpg)
![Immunohistochemistry-Paraffin: MTR Antibody [NBP3-41296] - DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-41296; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41296_rabbit-mtr-pab-612202421331646.jpg)
![Western Blot: ECH1 Antibody [NBP1-83216] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP1-83216-img0005.jpg "Western Blot: ECH1 Antibody [NBP1-83216] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83216] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunohistochemistry-Paraffin-NBP1-83216-img0002.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NBP1-89646] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP1-89646-img0008.jpg "Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NBP1-89646] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.")
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NBP1-89646] - Staining of human colon shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP1-89646-img0005.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NBP1-89646] - Staining of human kidney shows cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP1-89646-img0006.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NBP1-89646] - Staining of human placenta shows cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP1-89646-img0007.jpg)
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804] - Staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88804-img0006.jpg "Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804] - Staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.")
