| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95194-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37249-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80927-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38522-25ul | |||
![Western Blot: TORC2 Antibody [NBP3-12359] - 1:500 antibody dilution in DilObuffer.](http://images.novusbio.com/fullsize/TORC2-Antibody-Western-Blot-NBP3-12359-img0003.jpg "Western Blot: TORC2 Antibody [NBP3-12359] - 1:500 antibody dilution in DilObuffer.")
![Immunohistochemistry-Paraffin: TORC2 Antibody [NBP3-12359] - Mouse Spleen. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10X magnification.](http://images.novusbio.com/fullsize/TORC2-Antibody-Immunohistochemistry-Paraffin-NBP3-12359-img0001.jpg)
![Western Blot: TORC2 Antibody [NBP3-12359] - PC-TORC2 (1) and blueberry treated chondrocytes (2). 1:500 antibody dilution.](http://images.novusbio.com/fullsize/TORC2-Antibody-Western-Blot-NBP3-12359-img0002.jpg)
![Western Blot: PDK4 Antibody [NBP2-95194] - Western blot analysis of extracts of various cell lines, using PDK4 antibody (NBP2-95194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PDK4 Antibody-Western Blot-NBP2-95194-img0003.jpg "Western Blot: PDK4 Antibody [NBP2-95194] - Western blot analysis of extracts of various cell lines, using PDK4 antibody (NBP2-95194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-95194] - Immunohistochemistry of paraffin-embedded human placenta using PDK4 Rabbit pAb (NBP2-95194) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-95194-img0002.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-95194] - Immunohistochemistry of paraffin-embedded rat heart using PDK4 Rabbit pAb (NBP2-95194) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-95194-img0001.jpg)
![Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Western-Blot-NBP2-37249-img0003.jpg "Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Flow cytometric analysis of Hela cells using PPARGC1B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Flow-Cytometry-NBP2-37249-img0004.jpg)
![Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against human PPARGC1B (AA: 195-414) recombinant protein. (Expected MW is 50.3 kDa)](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Western-Blot-NBP2-37249-img0002.jpg)
![ELISA: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-ELISA-NBP2-37249-img0001.jpg)
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-29820241420941.jpg "Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32004) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-298202414262678.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Western blot analysis of PEDFR/PNPLA2/ATGL on different lysates with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 ug/Lane) Lane 2: HepG2 cell lysate (30 ug/Lane) Lane 3: HEK-293 cell lysate (30 ug/Lane) Lane 4: A431 cell lysate (30 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-392024848502.jpg)
![Western Blot: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32977) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-288202412493476.jpg "Western Blot: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32977) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32977) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-2982024149263.jpg)
![Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Immunocytochemistry analysis of HeLa cells labeling SREBP1 with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-29202410484870.jpg)
![Western Blot: FADS1 Antibody (2D9) [H00003992-M04] - Analysis of expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/FADS1-Antibody-2D9-Western-Blot-H00003992-M04-img0006.jpg "Western Blot: FADS1 Antibody (2D9) [H00003992-M04] - Analysis of expression in Hela S3 NE (Cat # L013V3).")
![Immunohistochemistry-Paraffin: FADS1 Antibody (2D9) [H00003992-M04] - Analysis of monoclonal antibody to FADS1 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/FADS1-Antibody-2D9-Immunohistochemistry-Paraffin-H00003992-M04-img0004.jpg)
![Sandwich ELISA: FADS1 Antibody (2D9) [H00003992-M04] - Detection limit for recombinant GST tagged FADS1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FADS1-Antibody-2D9-Sandwich-ELISA-H00003992-M04-img0005.jpg)
![Western Blot: PCK1 Antibody [NBP1-80927] - Analysis in human kidney tissue.](http://images.novusbio.com/fullsize/PCK1-Antibody-Western-Blot-NBP1-80927-img0006.jpg "Western Blot: PCK1 Antibody [NBP1-80927] - Analysis in human kidney tissue.")
![Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80927] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in renal tubules.](http://images.novusbio.com/fullsize/PCK1-Antibody-Immunohistochemistry-Paraffin-NBP1-80927-img0007.jpg)
![Western Blot: PCK1 Antibody [NBP1-80927] - Analysis in control (vector only transfected HEK293T lysate) and PCK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PCK1-Antibody-Western-Blot-NBP1-80927-img0008.jpg)
![Immunohistochemistry-Paraffin: TIGAR/C12orf5 Antibody [NBP2-38522] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-Immunohistochemistry-Paraffin-NBP2-38522-img0005.jpg "Immunohistochemistry-Paraffin: TIGAR/C12orf5 Antibody [NBP2-38522] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: TIGAR/C12orf5 Antibody [NBP2-38522] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-Immunohistochemistry-Paraffin-NBP2-38522-img0003.jpg)
![Immunohistochemistry-Paraffin: TIGAR/C12orf5 Antibody [NBP2-38522] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-Immunohistochemistry-Paraffin-NBP2-38522-img0004.jpg)
![Immunohistochemistry-Paraffin: TIGAR/C12orf5 Antibody [NBP2-38522] - Staining of human tonsil shows weak to moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-Immunohistochemistry-Paraffin-NBP2-38522-img0006.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32170-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for WB at 0.1ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32170-img0004.jpg)
![Western Blot: LPIN1 Antibody [NBP2-19370] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Western-Blot-NBP2-19370-img0001.jpg "Western Blot: LPIN1 Antibody [NBP2-19370] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Lipin 1 Antibody [NBP2-19370] - Paraffin-embedded hepatoma. LPIN1 antibody [C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/Lipin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-19370-img0003.jpg)
![Western Blot: IRS1 [p Ser307] Antibody [NBP1-78078] - Band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate. The pronounced staining of the band at 180 kDa is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction. The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).](http://images.novusbio.com/fullsize/IRS1-[p-Ser307]-Antibody-Western-Blot-NBP1-78078-img0001.jpg "Western Blot: IRS1 [p Ser307] Antibody [NBP1-78078] - Band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate. The pronounced staining of the band at 180 kDa is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction. The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).")
