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![Western Blot: Recombinant Rat Progranulin/PGRN Protein [NBP3-18148] - Deglycosylation of rat progranulin. To examine the deglycosylation of rat progranulin, 1ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37C. Separation of reaction products is visualized by immunoblotting using anti-progranulin (rat) antibody.](http://images.novusbio.com/fullsize/Recombinant-Rat-Progranulin-PGRN-Protein-Western-Blot-NBP3-18148-img0001.jpg "Western Blot: Recombinant Rat Progranulin/PGRN Protein [NBP3-18148] - Deglycosylation of rat progranulin. To examine the deglycosylation of rat progranulin, 1ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37C. Separation of reaction products is visualized by immunoblotting using anti-progranulin (rat) antibody.")
![Western Blot: PLA2G3 Antibody [H00050487-B01P] - Analysis of PLA2G3 expression in transfected 293T cell line by PLA2G3 polyclonal antibody. Lane 1: PLA2G3 transfected lysate(55.99 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PLA2G3-Antibody-Western-Blot-H00050487-B01P-img0001.jpg "Western Blot: PLA2G3 Antibody [H00050487-B01P] - Analysis of PLA2G3 expression in transfected 293T cell line by PLA2G3 polyclonal antibody. Lane 1: PLA2G3 transfected lysate(55.99 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: THTPA Antibody [H00079178-D01P] - Analysis of THTPA expression in transfected 293T cell line by THTPA polyclonal antibody.Lane 1: THTPA transfected lysate(25.60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/THTPA-Antibody-Western-Blot-H00079178-D01P-img0002.jpg "Western Blot: THTPA Antibody [H00079178-D01P] - Analysis of THTPA expression in transfected 293T cell line by THTPA polyclonal antibody.Lane 1: THTPA transfected lysate(25.60 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Cytochrome p450 2J2 Antibody [H00001573-B01P] - Analysis of CYP2J2 expression in transfected 293T cell line by CYP2J2 polyclonal antibody. Lane 1: CYP2J2 transfected lysate(55.22 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cytochrome-p450-2J2-Antibody-Western-Blot-H00001573-B01P-img0001.jpg "Western Blot: Cytochrome p450 2J2 Antibody [H00001573-B01P] - Analysis of CYP2J2 expression in transfected 293T cell line by CYP2J2 polyclonal antibody. Lane 1: CYP2J2 transfected lysate(55.22 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: RFK Antibody [H00055312-B01P] - Analysis of RFK expression in transfected 293T cell line by RFK polyclonal antibody. Lane 1: RFK transfected lysate(17.82 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RFK-Antibody-Western-Blot-H00055312-B01P-img0001.jpg "Western Blot: RFK Antibody [H00055312-B01P] - Analysis of RFK expression in transfected 293T cell line by RFK polyclonal antibody. Lane 1: RFK transfected lysate(17.82 KDa). Lane 2: Non-transfected lysate.")
![ELISA: Leptin/OB ELISA Kit [HRP] [KA0026] - Standard curve](http://images.novusbio.com/fullsize/Leptin-OB-ELISA-Kit-[HRP]-ELISA-KA0026-img0001.jpg "ELISA: Leptin/OB ELISA Kit [HRP] [KA0026] - Standard curve")
![Western Blot: Mannose Phosphate Isomerase Antibody [H00004351-B01P] - Analysis of MPI expression in transfected 293T cell line by MPI polyclonal antibody. Lane 1: MPI transfected lysate(46.53 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-Western-Blot-H00004351-B01P-img0002.jpg "Western Blot: Mannose Phosphate Isomerase Antibody [H00004351-B01P] - Analysis of MPI expression in transfected 293T cell line by MPI polyclonal antibody. Lane 1: MPI transfected lysate(46.53 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation:Sphingosine Kinase 1/SPHK1 AntibodyNBP3-29344] -Detection of human SPHK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPHK1 antibody NBP3-29344 used for IP at 10 µg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1., which recognizes a downstream epitope. For blotting immunoprecipitated SPHK1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Note: The more slowly migrating bands are believed to represent additional forms of SPHK1 owing to recognition by multiple antibodies each of which was directed toward distinctly different epitopes on SPHK1.](http://images.novusbio.com/fullsize/nbp3-29344_rabbit-sphingosine-kinase-1-sphk1-pab-301220241745127.jpg "Immunoprecipitation:Sphingosine Kinase 1/SPHK1 AntibodyNBP3-29344] -Detection of human SPHK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPHK1 antibody NBP3-29344 used for IP at 10 µg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1., which recognizes a downstream epitope. For blotting immunoprecipitated SPHK1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Note: The more slowly migrating bands are believed to represent additional forms of SPHK1 owing to recognition by multiple antibodies each of which was directed toward distinctly different epitopes on SPHK1.")