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![Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22 [p Ser397] Antibody-Western Blot-NBP1-44073-img0004.jpg "Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.")
![Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Western Blot: ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either a null vector (CTRL), ARHGAP22, ARHGAP22-serine 22 to alanine mutation, ARHGAP22 serine 397 to alanine mutation were electroblotted. The left panel was probed with the NBP1-44072 antibody, while the right panel was probed with the NBP1-44073 antibody, each at 1ug/ml. Each antibody recognizes the unmodified ARHGAP22. NBP1-44072 antibody recognized the S397->A mutation but not the S22->A mutation, while NBP1-44073 antibody recognizes the pS22->A mutation and not the pS397->A mutation. This data confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22-[p-Ser397]-Antibody-Western-Blot-NBP1-44073-img0001.jpg)
![Immunohistochemistry-Paraffin: ACAP4 Antibody [NBP2-24666] - Analysis of ASAP3 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/ACAP4-Antibody-Immunohistochemistry-Paraffin-NBP2-24666-img0001.jpg "Immunohistochemistry-Paraffin: ACAP4 Antibody [NBP2-24666] - Analysis of ASAP3 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.")
![Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22-[p-Ser22]-Antibody-Western-Blot-NBP1-44072-img0004.jpg "Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.")
![Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either a null vector (CTRL), ARHGAP22, ARHGAP22-serine 22 to alanine mutation, ARHGAP22 serine 397 to alanine mutation were electroblotted. The left panel was probed with the NBP1-44072 antibody, while the right panel was probed with the NBP1-44073 antibody, each at 1ug/ml. Each antibody recognizes the unmodified ARHGAP22. NBP1-44072 antibody recognized the S397-A mutation but not the S22-A mutation, while NBP1-44073 antibody recognizes the pS22-A mutation and not the pS397-A mutation. This data confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22-[p-Ser22]-Antibody-Western-Blot-NBP1-44072-img0001.jpg)
![Western Blot: NMNAT-1 Antibody [NBP2-32107] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-NMNAT1 antibody NBP2-32107 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NMNAT-1-Antibody-Western-Blot-NBP2-32107-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: NMNAT-1 Antibody [NBP2-32107] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-NMNAT1 antibody NBP2-32107 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-32107] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NMNAT1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NMNAT-1-Antibody-Immunohistochemistry-NBP2-32107-img0007.jpg)
![Western Blot: Importin-13 Antibody [NBP2-14994] - . Non-transfected (-) and Importin 13-transfected (+, ) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Importin 13 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Western-Blot-NBP2-14994-img0007.jpg "Western Blot: Importin-13 Antibody [NBP2-14994] - . Non-transfected (-) and Importin 13-transfected (+, ) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Importin 13 antibody diluted by 1:1000.")
![Immunocytochemistry/Immunofluorescence: Importin-13 Antibody [NBP2-14994] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14994-img0004.jpg)
![Immunohistochemistry-Paraffin: Importin-13 Antibody [NBP2-14994] - Paraffin-embedded MCF7 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Immunohistochemistry-Paraffin-NBP2-14994-img0005.jpg)
![Western Blot: CAD Antibody [NB100-61613] - Detection of Human and Mouse CAD on HeLa whole cell lysate using NB100-61613 . CAD was also immunoprecipitated by rabbit anti-CAD antibodies NB100-61612 and NB100-61614.](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NB100-61613-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CAD Antibody [NB100-61613] - Detection of Human and Mouse CAD on HeLa whole cell lysate using NB100-61613 . CAD was also immunoprecipitated by rabbit anti-CAD antibodies NB100-61612 and NB100-61614.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: CAD Antibody [NB100-61613] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-NB100-61613-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody [NBP2-15499] - HeLa cells mock (left) and treated with 2 ug/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATF4 protein stained by ATF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/ATF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15499-img0003.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATF4 Antibody [NBP2-15499] - HeLa cells mock (left) and treated with 2 ug/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATF4 protein stained by ATF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616] - Coommassie stained gel showing both free and HDL bound Apolipoprotein B/ApoB eluted from a solid phase resin prepared using anti-Human Apolipoprotein B/ApoB antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-Western-Blot-NB120-7616-img0001.jpg "Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616] - Coommassie stained gel showing both free and HDL bound Apolipoprotein B/ApoB eluted from a solid phase resin prepared using anti-Human Apolipoprotein B/ApoB antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.")
![Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NB120-7616] - Apolipoprotein B/ApoB antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-Immunohistochemistry-Paraffin-NB120-7616-img0002.jpg)
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Retinol Binding Protein RBP antibody (SPM442).](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-SPM442-Immunohistochemistry-Paraffin-NBP2-34362-img0001.jpg "Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Retinol Binding Protein RBP antibody (SPM442).")
![Western Blot: Phospholipase C delta 1 Antibody [NBP2-19810] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Western-Blot-NBP2-19810-img0005.jpg "Western Blot: Phospholipase C delta 1 Antibody [NBP2-19810] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/ Immunofluorescence: Phospholipase C delta 1 Antibody [NBP2-19810] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunocytochemistry--Immunofluorescence-NBP2-19810-img0004.jpg)
![Immunohistochemistry-Paraffin: Phospholipase C delta 1 Antibody [NBP2-19810] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-19810-img0002.jpg)
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (RBP/872) [NBP2-45015] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Retinol Binding Protein RBP](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-RBP-872-Immunohistochemistry-Paraffin-NBP2-45015-img0003.jpg "Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (RBP/872) [NBP2-45015] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Retinol Binding Protein RBP")
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (RBP/872) [NBP2-45015] - Human Hepatocellular Carcinoma stained with RBP1 (RBP/872)](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-RBP-872-Immunohistochemistry-Paraffin-NBP2-45015-img0001.jpg)
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (RBP/872) [NBP2-45015] - Human Ovarian Carcinoma stained with RBP1 (RBP/872)](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-RBP-872-Immunohistochemistry-Paraffin-NBP2-45015-img0002.jpg)
![Western Blot: ACAT2 Antibody [NBP2-32198] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ACAT2 antibody NBP2-32198 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Western-Blot-NBP2-32198-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ACAT2 Antibody [NBP2-32198] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ACAT2 antibody NBP2-32198 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- ACAT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-NBP2-32198-img0003.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- ACAT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-NBP2-32198-img0002.jpg)